LOCUS       Exported               12122 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of EGFR A859T.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-EGFR-A859T
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    161..278
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        326..1504
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          557..1495
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1452..1472
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1524..1548
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1551..5183
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLTKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5185..5209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5217..5235
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5260..5279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5261..5279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5285..5305)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5359..5400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5421..5988
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5438..5990
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5582..5599)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5809..5828
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6002..6601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6002..6021)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6498..6518
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6615..7203
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6668..6688)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7086..7097)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7278..7511
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7630..7648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7716..7820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7821..8681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8039..8058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8852..9440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9341..9360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9594..9611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9703..9722
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    9851..9872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9887..9917
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9925..9941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9930..9952
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9949..9965
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9949..9965
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10015..10034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10226..10859
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10906..11031
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11528..11761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11946..11990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
       61 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
      121 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      181 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      241 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      301 gagatccagt ttggactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
      361 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      421 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      481 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      541 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      601 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      661 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      721 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      781 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      841 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      901 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      961 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     1021 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     1081 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     1141 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     1201 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1261 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1321 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1381 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1441 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1501 gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa agcaggcacc atgcgaccct
     1561 ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg gcgagtcggg
     1621 ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag ttgggcactt
     1681 ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg gtccttggga
     1741 atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag accatccagg
     1801 aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct ttggaaaacc
     1861 tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca gtcttatcta
     1921 actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta caggaaatcc
     1981 tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag agcatccagt
     2041 ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc cagaaccacc
     2101 tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg ggtgcaggag
     2161 aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc gggcgctgcc
     2221 gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc acaggccccc
     2281 gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc aaggacacct
     2341 gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac cccgagggca
     2401 aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg gtgacagatc
     2461 acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa gacggcgtcc
     2521 gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata ggtattggtg
     2581 aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa aactgcacct
     2641 ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc ttcacacata
     2701 ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa atcacagggt
     2761 ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt gagaacctag
     2821 aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc gtcagcctga
     2881 acataacatc cttgggatta cgctccctca aggagataag tgatggagat gtgataattt
     2941 caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg tttgggacct
     3001 ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag gccacaggcc
     3061 aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc agggactgcg
     3121 tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac cttctggagg
     3181 gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca gagtgcctgc
     3241 ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc cagtgtgccc
     3301 actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg ggagaaaaca
     3361 acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc catccaaact
     3421 gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg cctaagatcc
     3481 cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg gccctgggga
     3541 tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg aggctgctgc
     3601 aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac caagctctct
     3661 tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc ggtgcgttcg
     3721 gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt cccgtcgcta
     3781 tcaaggaatt aagagaagca acatctccga aagccaacaa ggaaatcctc gatgaagcct
     3841 acgtgatggc cagcgtggac aacccccacg tgtgccgcct gctgggcatc tgcctcacct
     3901 ccaccgtgca gctcatcacg cagctcatgc ccttcggctg cctcctggac tatgtccggg
     3961 aacacaaaga caatattggc tcccagtacc tgctcaactg gtgtgtgcag atcgcaaagg
     4021 gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc aggaacgtac
     4081 tggtgaaaac accgcagcat gtcaagatca cagattttgg gctgaccaaa ctgctgggtg
     4141 cggaagagaa agaataccat gcagaaggag gcaaagtgcc tatcaagtgg atggcattgg
     4201 aatcaatttt acacagaatc tatacccacc agagtgatgt ctggagctac ggggtgaccg
     4261 tttgggagtt gatgaccttt ggatccaagc catatgacgg aatccctgcc agcgagatct
     4321 cctccatcct ggagaaagga gaacgcctcc ctcagccacc catatgtacc atcgatgtct
     4381 acatgatcat ggtcaagtgc tggatgatag acgcagatag tcgcccaaag ttccgtgagt
     4441 tgatcatcga attctccaaa atggcccgag acccccagcg ctaccttgtc attcaggggg
     4501 atgaaagaat gcatttgcca agtcctacag actccaactt ctaccgtgcc ctgatggatg
     4561 aagaagacat ggacgacgtg gtggatgccg acgagtacct catcccacag cagggcttct
     4621 tcagcagccc ctccacgtca cggactcccc tcctgagctc tctgagtgca accagcaaca
     4681 attccaccgt ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc aaggaagaca
     4741 gcttcttgca gcgatacagc tcagacccca caggcgcctt gactgaggac agcatagacg
     4801 acaccttcct cccagtgcct gaatacataa accagtccgt tcccaaaagg cccgctggct
     4861 ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc agagacccac
     4921 actaccagga cccccacagc actgcagtgg gcaaccccga gtatctcaac actgtccagc
     4981 ccacctgtgt caacagcaca ttcgacagcc ctgcccactg ggcccagaaa ggcagccacc
     5041 aaattagcct ggacaaccct gactaccagc aggacttctt tcccaaggaa gccaagccaa
     5101 atggcatctt taagggctcc acagctgaaa atgcagaata cctaagggtc gcgccacaaa
     5161 gcagtgaatt tattggagca tagaacccag ctttcttgta caaagtggtg atatccaatt
     5221 aaccctcact aaagggtaat aagttgtgaa agaagattac cctatagtga gtcgtattac
     5281 cacccaactt ttctatacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     5341 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     5401 cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg
     5461 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5521 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5581 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5641 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5701 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5761 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5821 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5881 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5941 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca
     6001 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6061 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6121 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6181 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6241 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6301 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6361 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6421 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6481 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6541 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta
     6601 aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6661 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6721 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6781 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6841 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6901 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6961 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7021 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7081 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7141 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7201 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7261 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7321 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7381 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7441 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7501 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7561 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     7621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8041 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     8101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9661 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
     9721 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9781 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9841 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9901 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9961 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10021 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10081 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    10141 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10201 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    10261 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    10321 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    10381 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    10441 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    10501 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    10561 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    10621 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    10681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10861 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10921 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10981 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11041 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11101 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11161 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11221 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11281 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11341 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
    11401 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    11461 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    11521 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    11581 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    11641 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    11701 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    11761 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    11821 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    11881 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    11941 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    12001 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    12061 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    12121 ca
//
