LOCUS Exported 10790 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of BRAF S637*. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-S637* SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..1179 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 232..1170 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1127..1147 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1199..1223 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1224..1233 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3528..3552) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3560..3578 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3603..3622) /label=T7 /note="T7 promoter, forward primer" promoter complement(3604..3622) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(3628..3648) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 3702..3743 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3764..4331 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3781..4333 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3925..3942) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4152..4171 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4345..4944 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4345..4364) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4841..4861 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 4958..5546 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5011..5031) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5429..5440) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5621..5854 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(5973..5991) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6059..6163 /gene="bla" /label=AmpR promoter CDS 6164..7024 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6382..6401) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7195..7783 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7684..7703 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7937..7954 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8046..8065 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8194..8215 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8230..8260 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8268..8284 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8273..8295 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8292..8308 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8292..8308 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8358..8377 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8569..9202 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9249..9374 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9871..10104 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10289..10333 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10626..10743 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac 1201 aagtttgtac aaaaaagcag gcaccatggc ggcgctgagc ggtggcggtg gtggcggcgc 1261 ggagccgggc caggctctgt tcaacgggga catggagccc gaggccggcg ccggcgccgg 1321 cgccgcggcc tcttcggctg cggaccctgc cattccggag gaggtgtgga atatcaaaca 1381 aatgattaag ttgacacagg aacatataga ggccctattg gacaaatttg gtggggagca 1441 taatccacca tcaatatatc tggaggccta tgaagaatac accagcaagc tagatgcact 1501 ccaacaaaga gaacaacagt tattggaatc tctggggaac ggaactgatt tttctgtttc 1561 tagctctgca tcaatggata ccgttacatc ttcttcctct tctagccttt cagtgctacc 1621 ttcatctctt tcagtttttc aaaatcccac agatgtggca cggagcaacc ccaagtcacc 1681 acaaaaacct atcgttagag tcttcctgcc caacaaacag aggacagtgg tacctgcaag 1741 gtgtggagtt acagtccgag acagtctaaa gaaagcactg atgatgagag gtctaatccc 1801 agagtgctgt gctgtttaca gaattcagga tggagagaag aaaccaattg gttgggacac 1861 tgatatttcc tggcttactg gagaagaatt gcatgtggaa gtgttggaga atgttccact 1921 tacaacacac aactttgtac gaaaaacgtt tttcacctta gcattttgtg acttttgtcg 1981 aaagctgctt ttccagggtt tccgctgtca aacatgtggt tataaatttc accagcgttg 2041 tagtacagaa gttccactga tgtgtgttaa ttatgaccaa cttgatttgc tgtttgtctc 2101 caagttcttt gaacaccacc caataccaca ggaagaggcg tccttagcag agactgccct 2161 aacatctgga tcatcccctt ccgcacccgc ctcggactct attgggcccc aaattctcac 2221 cagtccgtct ccttcaaaat ccattccaat tccacagccc ttccgaccag cagatgaaga 2281 tcatcgaaat caatttgggc aacgagaccg atcctcatca gctcccaatg tgcatataaa 2341 cacaatagaa cctgtcaata ttgatgactt gattagagac caaggatttc gtggtgatgg 2401 aggatcaacc acaggtttgt ctgctacccc ccctgcctca ttacctggct cactaactaa 2461 cgtgaaagcc ttacagaaat ctccaggacc tcagcgagaa aggaagtcat cttcatcctc 2521 agaagacagg aatcgaatga aaacacttgg tagacgggac tcgagtgatg attgggagat 2581 tcctgatggg cagattacag tgggacaaag aattggatct ggatcatttg gaacagtcta 2641 caagggaaag tggcatggtg atgtggcagt gaaaatgttg aatgtgacag cacctacacc 2701 tcagcagtta caagccttca aaaatgaagt aggagtactc aggaaaacac gacatgtgaa 2761 tatcctactc ttcatgggct attccacaaa gccacaactg gctattgtta cccagtggtg 2821 tgagggctcc agcttgtatc accatctcca tatcattgag accaaatttg agatgatcaa 2881 acttatagat attgcacgac agactgcaca gggcatggat tacttacacg ccaagtcaat 2941 catccacaga gacctcaaga gtaataatat atttcttcat gaagacctca cagtaaaaat 3001 aggtgatttt ggtctagcta cagtgaaatc tcgatggagt gggtcccatc agtttgaaca 3061 gttgtctgga tccattttgt ggatggcacc agaagtcatc agaatgcaag ataaaaatcc 3121 atacagcttt cagtgagatg tatatgcatt tggaattgtt ctgtatgaat tgatgactgg 3181 acagttacct tattcaaaca tcaacaacag ggaccagata atttttatgg tgggacgagg 3241 atacctgtct ccagatctca gtaaggtacg gagtaactgt ccaaaagcca tgaagagatt 3301 aatggcagag tgcctcaaaa agaaaagaga tgagagacca ctctttcccc aaattctcgc 3361 ctctattgag ctgctggccc gctcattgcc aaaaattcac cgcagtgcat cagaaccctc 3421 cttgaatcgg gctggtttcc aaacagagga ttttagtcta tatgcttgtg cttctccaaa 3481 aacacccatc caggcagggg gatatggtgc gtttcctgtc cactagaacc cagctttctt 3541 gtacaaagtg gtgatatcca attaaccctc actaaaggga aagtttaagt gatgtatatt 3601 gtccctatag tgagtcgtat taccacccaa cttttctata caaagtggtt gatatccagc 3661 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc 3721 ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agatccctcc ccccccccta 3781 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 3841 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 3901 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 3961 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 4021 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 4081 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 4141 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 4201 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 4261 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 4321 cacgatgata atatggccac acatatgacc gagtacaagc ccacggtgcg cctcgccacc 4381 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4441 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4501 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4561 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4621 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4681 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4741 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4801 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 4861 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 4921 atgacccgca agcccggtgc ctaaatcgat agatcctaat caacctctgg attacaaaat 4981 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5041 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5101 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5161 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5221 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5281 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5341 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct 5401 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5461 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 5521 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca 5581 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 5641 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 5701 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 5761 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 5821 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 5881 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag 5941 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt 6001 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 6061 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 6121 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 6181 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 6241 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 6301 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 6361 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca 6421 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 6481 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 6541 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 6601 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 6661 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 6721 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 6781 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 6841 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 6901 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 6961 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 7021 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 7081 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 7141 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 7201 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 7261 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 7321 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 7381 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 7441 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 7501 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 7561 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 7621 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 7681 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 7741 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 7801 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 7861 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 7921 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 7981 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt 8041 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 8101 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc 8161 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 8221 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 8281 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt 8341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 8401 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac 8461 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc 8521 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa 8581 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 8641 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat 8701 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga 8761 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt 8821 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg 8881 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc 8941 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag 9001 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 9061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 9121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 9181 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac 9241 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg 9301 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg 9361 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt 9421 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc 9481 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac 9541 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata 9601 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt 9661 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc 9721 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa 9781 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt 9841 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc 9901 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt 9961 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct 10021 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag 10081 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac 10141 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca 10201 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt 10261 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa 10321 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt 10381 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt 10441 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag 10501 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc 10561 cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc 10621 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac 10681 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat 10741 tttcgggttt attacaggga cagcagagat ccagtttgga ctagtcgtga //