LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR E709A. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-E709A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 110..227 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 275..1453 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 506..1444 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1401..1421 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1473..1497 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1500..5132 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKATEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5134..5158) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5166..5184 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5209..5228) /label=T7 /note="T7 promoter, forward primer" promoter complement(5210..5228) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5234..5254) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5308..5349 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5370..5937 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5387..5939 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5531..5548) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5758..5777 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5951..6550 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5951..5970) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6447..6467 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6564..7152 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6617..6637) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7035..7046) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7227..7460 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7579..7597) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7665..7769 /gene="bla" /label=AmpR promoter CDS 7770..8630 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7988..8007) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8801..9389 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9290..9309 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9543..9560 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9652..9671 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9800..9821 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9836..9866 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9874..9890 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9879..9901 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9898..9914 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9898..9914 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9964..9983 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10175..10808 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10855..10980 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11477..11710 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11895..11939 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 61 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa 121 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 181 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca 241 gggacagcag agatccagtt tggactagtc gtgaggctcc ggtgcccgtc agtgggcaga 301 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 361 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 421 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 481 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 541 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat 601 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg 661 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg 721 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa 781 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca 841 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc 901 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg 961 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc 1021 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct 1081 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg 1141 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc 1201 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc 1261 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga 1321 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga 1381 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt 1441 tcaggtgtcg tgaagcggcc ctgcagatat caacaagttt gtacaaaaaa gcaggcacca 1501 tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg 1561 cgagtcgggc tctggaggaa aagaaagttt gccaaggcac gagtaacaag ctcacgcagt 1621 tgggcacttt tgaagatcat tttctcagcc tccagaggat gttcaataac tgtgaggtgg 1681 tccttgggaa tttggaaatt acctatgtgc agaggaatta tgatctttcc ttcttaaaga 1741 ccatccagga ggtggctggt tatgtcctca ttgccctcaa cacagtggag cgaattcctt 1801 tggaaaacct gcagatcatc agaggaaata tgtactacga aaattcctat gccttagcag 1861 tcttatctaa ctatgatgca aataaaaccg gactgaagga gctgcccatg agaaatttac 1921 aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga 1981 gcatccagtg gcgggacata gtcagcagtg actttctcag caacatgtcg atggacttcc 2041 agaaccacct gggcagctgc caaaagtgtg atccaagctg tcccaatggg agctgctggg 2101 gtgcaggaga ggagaactgc cagaaactga ccaaaatcat ctgtgcccag cagtgctccg 2161 ggcgctgccg tggcaagtcc cccagtgact gctgccacaa ccagtgtgct gcaggctgca 2221 caggcccccg ggagagcgac tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca 2281 aggacacctg ccccccactc atgctctaca accccaccac gtaccagatg gatgtgaacc 2341 ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg 2401 tgacagatca cggctcgtgc gtccgagcct gtggggccga cagctatgag atggaggaag 2461 acggcgtccg caagtgtaag aagtgcgaag ggccttgccg caaagtgtgt aacggaatag 2521 gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa 2581 actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg ggtgactcct 2641 tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc gtaaaggaaa 2701 tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc catgcctttg 2761 agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct cttgcagtcg 2821 tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg 2881 tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg aaaaaactgt 2941 ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac agctgcaagg 3001 ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc ccggagccca 3061 gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc 3121 ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag 3181 agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac aactgtatcc 3241 agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca ggagtcatgg 3301 gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc 3361 atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca acgaatgggc 3421 ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg 3481 ccctggggat cggcctcttc atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga 3541 ggctgctgca ggagagggag cttgtggagc ctcttacacc cagtggagaa gctcccaacc 3601 aagctctctt gaggatcttg aaggcaactg aattcaaaaa gatcaaagtg ctgggctccg 3661 gtgcgttcgg cacggtgtat aagggactct ggatcccaga aggtgagaaa gttaaaattc 3721 ccgtcgctat caaggaatta agagaagcaa catctccgaa agccaacaag gaaatcctcg 3781 atgaagccta cgtgatggcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct 3841 gcctcacctc caccgtgcag ctcatcacgc agctcatgcc cttcggctgc ctcctggact 3901 atgtccggga acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga 3961 tcgcaaaggg catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca 4021 ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac 4081 tgctgggtgc ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga 4141 tggcattgga atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg 4201 gggtgaccgt ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca 4261 gcgagatctc ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca 4321 tcgatgtcta catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt 4381 tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca 4441 ttcaggggga tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc 4501 tgatggatga agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc 4561 agggcttctt cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa 4621 ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca 4681 aggaagacag cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca 4741 gcatagacga caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc 4801 ccgctggctc tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca 4861 gagacccaca ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca 4921 ctgtccagcc cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag 4981 gcagccacca aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag 5041 ccaagccaaa tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg 5101 cgccacaaag cagtgaattt attggagcat agaacccagc tttcttgtac aaagtggtga 5161 tatccaatta accctcacta aagggttatg tagagatgaa agaaattacc ctatagtgag 5221 tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag tggcggccgc 5281 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc 5341 gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga 5401 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 5461 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 5521 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 5581 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 5641 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 5701 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 5761 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 5821 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 5881 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat 5941 ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 6001 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 6061 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 6121 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 6181 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 6241 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 6301 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 6361 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 6421 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 6481 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 6541 cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga 6601 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 6661 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 6721 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 6781 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 6841 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 6901 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 6961 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 7021 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 7081 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 7141 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag 7201 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 7261 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 7321 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 7381 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 7441 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 7501 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc 7561 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 7621 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 7681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 7801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 7861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 7921 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 7981 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 8041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 8101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 8161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 8221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 8281 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 8341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 8401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 8461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 8521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 8581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 8641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 8761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 8821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 8881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 8941 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 9001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 9061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 9121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 9181 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 9241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 9301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 9361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 9421 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 9481 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 9541 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 9601 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc 9661 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 9721 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg 9781 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 9841 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 9901 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta 9961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc 10021 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc 10081 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt 10141 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga 10201 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag 10261 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta 10321 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta 10381 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt 10441 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac 10501 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc 10561 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc 10621 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 10681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 10741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 10801 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 10861 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 10921 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 10981 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 11041 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 11101 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 11161 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 11221 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 11281 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac 11341 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa 11401 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa 11461 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta 11521 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc 11581 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag 11641 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc 11701 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt 11761 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg 11821 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc 11881 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt 11941 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag 12001 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc 12061 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc 12121 cc //