LOCUS Exported 9704 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of STK19 D89N. ACCESSION . VERSION . KEYWORDS pHAGE-STK19-D89N SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9704) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9704) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9704 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4544..4568) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4576..4594 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4619..4638) /label=T7 /note="T7 promoter, forward primer" promoter complement(4620..4638) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(4644..4664) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 4718..4759 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4780..5347 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4797..5349 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4941..4958) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5168..5187 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5361..6080 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5361..6077 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5406..5427) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5667..5686) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6014..6035 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6094..6682 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6147..6167) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6565..6576) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6757..6990 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7109..7127) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7195..7299 /gene="bla" /label=AmpR promoter CDS 7300..8160 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7518..7537) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8331..8919 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8820..8839 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9073..9090 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9182..9201 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9330..9351 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9366..9396 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9404..9420 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9409..9431 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9428..9444 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9428..9444 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9494..9513 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg caaaagtggt tttctgcttt cgatgatgca 3481 atcattcagc gacagtggcg ggcaaacccc tcccggggcg ggggaggtgt gagcttcacg 3541 aaggaggttg acaccaacgt ggccaccggc gcccctccac gccgccaacg agtccccggg 3601 cgtgcgtgcc cttggaggga gccaatccgc ggccggcgtg gggcccggcc tggcggaggt 3661 gatgctggag ggacgcccgg ggagaccgta cgtcactgct ctgcgccgga aaaccctatt 3721 ttcaggttct cttccctcca ttcctacccc ttccccggta ccataaaatc ccgggatatg 3781 agctggaaga ggcatcacct gatcccggag acctttggag ttaagaggcg gcggaagcga 3841 gggcctgtgg agtcggatcc tcttcggggt gagccagggt cggcgcgcgc ggctgtctca 3901 gaactcatgc agctgttccc gcgaggcctg tttgaggacg cgctgccgcc catcgtgctg 3961 aggagccagg tgtacagcct tgtgcctgac aggaccgtgg ccgaccggca gctgaaggag 4021 cttcaagagc agggggagat cagaatcgtc cagctgggct tcgacttgga tgcccatgga 4081 attatcttca ctgaggacta caggaccaga gtcctcaagg cctgtgatgg ccgaccgtat 4141 gctggggcag tgcagaaatt tctagcttca gtacttccag cctgtgggga ccttagtttc 4201 cagcaggacc aaatgacaca gacctttggc ttcagggact cagaaatcac gcatctggtg 4261 aatgctggag tcctcaccgt ccgagatgct gggagctggt ggctagctgt gcctggagct 4321 gggagattca tcaagtactt tgttaaaggg cgccaggctg tccttagcat ggtccggaag 4381 gcaaagtacc gggaactgct cctatcagag ctcctgggcc ggcgggcgcc tgtcgtggtg 4441 cggcttggcc tcacctacca tgtgcacgac ctcattgggg cccagctagt ggactgcatc 4501 tctaccactt caggaaccct cctccgcctg ccagagacat agaacccagc tttcttgtac 4561 aaagtggtga tatccaatta accctcacta aaggtgtagt gtttagagta agttattacc 4621 ctatagtgag tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag 4681 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct 4741 cctcggtctc gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt 4801 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac 4861 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag 4921 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa 4981 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag 5041 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga 5101 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag 5161 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc 5221 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag 5281 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 5341 atgataatat ggccacacat atggtgagca agggcgagga gctgttcacc ggggtggtgc 5401 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5461 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5521 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 5581 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 5641 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 5701 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 5761 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 5821 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 5881 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 5941 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 6001 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 6061 tggacgagct gtacaagtaa atcgatagat cctaatcaac ctctggatta caaaatttgt 6121 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 6181 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6241 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6301 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6361 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6421 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6481 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 6541 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6601 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 6661 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg 6721 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 6781 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 6841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 6901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 6961 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 7021 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa 7081 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat 7141 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 7201 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 7261 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 7321 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 7381 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 7441 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 7501 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag 7561 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 7621 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 7681 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 7741 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 7801 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 7861 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 7921 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 7981 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 8041 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 8101 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 8161 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 8221 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 8281 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 8341 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 8401 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 8461 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 8521 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 8581 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 8641 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 8701 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 8761 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 8821 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 8881 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 8941 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 9001 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 9061 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 9121 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt 9181 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 9241 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac 9301 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 9361 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 9421 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca 9481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 9541 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 9601 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 9661 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //