LOCUS Exported 2341 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 0 part. Promoter. ACCESSION . VERSION . KEYWORDS pC0.033 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2341) AUTHORS Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick AJ TITLE CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax. JOURNAL Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub 2019 Feb 28. PUBMED 30819783 REFERENCE 2 (bases 1 to 2341) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2341 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(24..42) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 110..214 /gene="bla" /label=AmpR promoter CDS 436..1227 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1398..1986 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1887..1906 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2140..2157 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2275..2291 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 2309..2320 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" ORIGIN 1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac 241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg 361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa 421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag 481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc 541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc 601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct 661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac 721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat 781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg 841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat 901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg 961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac 1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc 1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa 1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc 1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag 1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca 2221 ctctgtggtc tcaggagctg atagctagct cagtcctagg gattatgcta gctattgtga 2281 gcggataaca atttcacaca tactagagaa agaggagaaa tactaaatgt gagaccacga 2341 a //