LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR E709Q. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-E709Q SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 218..335 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 383..1561 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 614..1552 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1509..1529 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1581..1605 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1608..5240 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKQTEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5242..5266) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5274..5292 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5317..5336) /label=T7 /note="T7 promoter, forward primer" promoter complement(5318..5336) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5342..5362) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5416..5457 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5478..6045 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5495..6047 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5639..5656) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5866..5885 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6059..6658 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6059..6078) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6555..6575 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6672..7260 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6725..6745) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7143..7154) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7335..7568 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7687..7705) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7773..7877 /gene="bla" /label=AmpR promoter CDS 7878..8738 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8096..8115) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8909..9497 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9398..9417 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9651..9668 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9760..9779 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9908..9929 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9944..9974 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9982..9998 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9987..10009 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10006..10022 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10006..10022 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10072..10091 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10283..10916 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10963..11088 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11585..11818 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12003..12047 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 61 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 121 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg 181 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg 241 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag 301 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag 361 atccagtttg gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc 421 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg 481 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 541 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 601 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta 661 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga 721 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 781 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 841 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc 901 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 961 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 1021 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 1081 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 1141 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 1201 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 1261 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 1321 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 1381 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 1441 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 1501 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 1561 aagcggccct gcagatatca acaagtttgt acaaaaaagc aggcaccatg cgaccctccg 1621 ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc 1681 tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg ggcacttttg 1741 aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc cttgggaatt 1801 tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc atccaggagg 1861 tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg gaaaacctgc 1921 agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc ttatctaact 1981 atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag gaaatcctgc 2041 atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc atccagtggc 2101 gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag aaccacctgg 2161 gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt gcaggagagg 2221 agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg cgctgccgtg 2281 gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca ggcccccggg 2341 agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc 2401 ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc gagggcaaat 2461 acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg 2521 gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac ggcgtccgca 2581 agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt attggtgaat 2641 ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac tgcacctcca 2701 tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc acacatactc 2761 ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc acagggtttt 2821 tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag aacctagaaa 2881 tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc agcctgaaca 2941 taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg ataatttcag 3001 gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt gggacctccg 3061 gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc acaggccagg 3121 tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg gactgcgtct 3181 cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg 3241 agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag tgcctgcctc 3301 aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag tgtgcccact 3361 acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga gaaaacaaca 3421 ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat ccaaactgca 3481 cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct aagatcccgt 3541 ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc ctggggatcg 3601 gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg 3661 agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa gctctcttga 3721 ggatcttgaa gcaaactgaa ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca 3781 cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc gtcgctatca 3841 aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat gaagcctacg 3901 tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc ctcacctcca 3961 ccgtgcagct catcacgcag ctcatgccct tcggctgcct cctggactat gtccgggaac 4021 acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc gcaaagggca 4081 tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg aacgtactgg 4141 tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg ctgggtgcgg 4201 aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg gcattggaat 4261 caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg gtgaccgttt 4321 gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc gagatctcct 4381 ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc gatgtctaca 4441 tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc cgtgagttga 4501 tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt cagggggatg 4561 aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg atggatgaag 4621 aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag ggcttcttca 4681 gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc agcaacaatt 4741 ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag gaagacagct 4801 tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc atagacgaca 4861 ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc gctggctctg 4921 tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga gacccacact 4981 accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact gtccagccca 5041 cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc agccaccaaa 5101 ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc aagccaaatg 5161 gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg ccacaaagca 5221 gtgaatttat tggagcatag aacccagctt tcttgtacaa agtggtgata tccaattaac 5281 cctcactaaa ggattgattt gttgaaataa gtgttgccct atagtgagtc gtattaccac 5341 ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag 5401 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt 5461 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 5521 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 5581 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 5641 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 5701 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 5761 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 5821 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 5881 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 5941 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 6001 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat 6061 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 6121 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 6181 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 6241 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 6301 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 6361 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 6421 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 6481 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 6541 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 6601 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat 6661 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 6721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 6781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 6841 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 6901 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 6961 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 7021 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 7081 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 7141 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 7201 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 7261 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 7321 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 7381 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 7441 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7501 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7561 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7621 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac 7681 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 7741 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 7801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 7861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 7921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 7981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 8041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 8101 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact 8161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 8221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 8281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 8341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 8401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 8461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 8521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 8581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 8641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 8701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 8761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 8821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 8881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 8941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 9001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 9061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 9121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 9181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 9241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 9301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 9361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 9421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 9481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 9541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 9601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 9661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 9721 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc 9781 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 9841 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 9901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 9961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 10021 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 10081 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa 10141 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt 10201 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa 10261 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc 10321 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca 10381 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag 10441 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc 10501 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta 10561 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga 10621 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact 10681 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc 10741 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 10801 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 10861 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 10921 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 10981 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 11041 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 11101 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 11161 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 11221 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 11281 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 11341 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 11401 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag 11461 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat 11521 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg 11581 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt 11641 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca 11701 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca 11761 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg 11821 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt 11881 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag 11941 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag 12001 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta 12061 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag 12121 gt //