LOCUS Exported 10283 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of TBC1D3B G164E. ACCESSION . VERSION . KEYWORDS pHAGE-TBC1D3B-G164E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10283) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10283) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10283 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5123..5147) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5155..5173 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5198..5217) /label=T7 /note="T7 promoter, forward primer" promoter complement(5199..5217) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5223..5243) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5297..5338 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5359..5926 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5376..5928 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5520..5537) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5747..5766 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5940..6659 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5940..6656 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5985..6006) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6246..6265) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6593..6614 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6673..7261 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6726..6746) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7144..7155) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7336..7569 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7688..7706) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7774..7878 /gene="bla" /label=AmpR promoter CDS 7879..8739 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8097..8116) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8910..9498 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9399..9418 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9652..9669 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9761..9780 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9909..9930 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9945..9975 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9983..9999 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9988..10010 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10007..10023 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10007..10023 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10073..10092 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatggacgt ggtagaggtc 3481 gcgggtagtt ggtgggcaca agagcgagag gacatcatta tgaaatacga aaagggacac 3541 cgagctgggc tgccagagga caaggggcct aagccttttc gaagctacaa caacaacgtc 3601 gatcatttgg ggattgtaca tgagacggag ctgcctcctc tgactgcgcg ggaggcgaag 3661 caaattcggc gggagatcag ccgaaagagc aagtgggtgg atatgctggg agactgggag 3721 aaatacaaaa gcagcagaaa gctcatagat cgagcgtaca agggaatgcc catgaacatc 3781 cggggcccga tgtggtcagt cctcctgaac attgaggaaa tgaagttgaa aaaccccgga 3841 agataccaga tcatgaagga gaagggcaag aggtcatctg agcacatcca gcgcatcgac 3901 cgggacataa gcgggacatt aaggaagcat atgttcttca gggatcgata cgaaaccaag 3961 cagcgggaac tactccacat cctcctggca tatgaggagt ataacccgga ggtgggctac 4021 tgcagggacc tgagccacat cgccgccttg ttcctcctct attttcctga ggaggatgca 4081 ttctgggcac tggtgcagct gctggccagt gagaggcact ccctgcaggg atttcacagc 4141 ccaaatggcg ggaccgtcca ggggctccaa gaccaacagg agcatgtggt agccacgtca 4201 caatccaaga ccatggggca tcaggacaag aaagatctat gtgggcagtg ttccccgtta 4261 ggctgcctca tccggatatt gattgacggg atctctctcg ggctcaccct gcgcctgtgg 4321 gacgtgtatc tggtagaagg cgaacaggcg ttgatgccga taacaagaat cgcctttaag 4381 gttcagcaga agcgcctcac gaagacgtcc aggtgtggcc cgtgggcacg tttttgcaac 4441 cggttcgttg atacctgggc cagggatgag gacactgtgc tcaagcatct tagggcctct 4501 atgaagaaac taacaagaaa gcagggggac ctgccacccc cagccaaacc cgagcaaggg 4561 tcgtcggcat ccaggcctgt gccggcttca cgtggcggga agaccctctg caagggggac 4621 aggcaggccc ctccaggccc accagcccgg ttcccgcggc ccatttggtc agcttccccg 4681 ccacgggcac ctcgttcttc cacaccctgt cctggtgggg ctgtccggga agacacctac 4741 cctgtgggca ctcagggtgt gcccagcccg gccctggctc agggaggacc tcagggttcc 4801 tggagattcc tgcagtggaa ctccatgccc cgcctcccaa cggacctgga cgtagagggc 4861 ccttggttcc gccattatga tttcagacag agctgctggg tccgtgccat atcccaggag 4921 gaccagctgg ccccctgctg gcaggctgaa caccctgcgg agcgggtgag atcggctttc 4981 gctgcaccca gcactgattc cgaccagggc acccccttca gagctaggga cgaacagcag 5041 tatgctccca cctcagggcc ttgcctctgc ggcctccact tggaaagttc tcagttccct 5101 ccaggcttct agggcctcat gggcccagct ttcttgtaca aagtggtgat atccaattaa 5161 ccctcactaa aggatgtgtt tatgtgaaag atttgtaccc tatagtgagt cgtattacca 5221 cccaactttt ctatacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga 5281 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg 5341 taccggttag taatgagatc cctccccccc ccctaacgtt actggccgaa gccgcttgga 5401 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 5461 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 5521 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 5581 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 5641 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 5701 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 5761 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 5821 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 5881 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata 5941 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 6001 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 6061 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 6121 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 6181 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 6241 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 6301 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 6361 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 6421 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 6481 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 6541 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 6601 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa 6661 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 6721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 6781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 7021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 7081 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 7141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 7201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 7261 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7321 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 7381 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 7441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 7501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 7561 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 7621 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga 7681 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 7741 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 7801 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 7861 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 7921 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 7981 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 8041 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 8101 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac 8161 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 8221 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 8281 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 8341 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 8401 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 8461 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 8521 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 8581 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 8641 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 8701 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 8761 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 8821 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 8881 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 8941 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 9001 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 9061 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 9121 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 9181 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 9241 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 9301 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 9361 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 9421 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 9481 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 9541 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 9601 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 9661 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 9721 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg 9781 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 9841 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 9901 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 9961 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 10021 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 10081 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa 10141 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat 10201 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa 10261 atatgtacta caaacttagt agt //