LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR E804G. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-E804G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 154..271 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 319..1497 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 550..1488 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1445..1465 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1517..1541 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1544..5176 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVRGHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5178..5202) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5210..5228 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5253..5272) /label=T7 /note="T7 promoter, forward primer" promoter complement(5254..5272) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5278..5298) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5352..5393 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5414..5981 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5431..5983 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5575..5592) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5802..5821 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5995..6594 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5995..6014) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6491..6511 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6608..7196 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6661..6681) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7079..7090) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7271..7504 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7623..7641) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7709..7813 /gene="bla" /label=AmpR promoter CDS 7814..8674 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8032..8051) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8845..9433 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9334..9353 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9587..9604 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9696..9715 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9844..9865 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9880..9910 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9918..9934 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9923..9945 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9942..9958 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9942..9958 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10008..10027 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10219..10852 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10899..11024 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11521..11754 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11939..11983 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 61 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc 121 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg 181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt 241 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc 301 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 361 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 421 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 481 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 541 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 601 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt 661 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 721 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 781 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 841 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 901 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 961 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 1021 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 1081 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 1141 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 1201 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 1261 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 1321 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 1381 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 1441 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc 1501 ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc accatgcgac cctccgggac 1561 ggccggggca gcgctcctgg cgctgctggc tgcgctctgc ccggcgagtc gggctctgga 1621 ggaaaagaaa gtttgccaag gcacgagtaa caagctcacg cagttgggca cttttgaaga 1681 tcattttctc agcctccaga ggatgttcaa taactgtgag gtggtccttg ggaatttgga 1741 aattacctat gtgcagagga attatgatct ttccttctta aagaccatcc aggaggtggc 1801 tggttatgtc ctcattgccc tcaacacagt ggagcgaatt cctttggaaa acctgcagat 1861 catcagagga aatatgtact acgaaaattc ctatgcctta gcagtcttat ctaactatga 1921 tgcaaataaa accggactga aggagctgcc catgagaaat ttacaggaaa tcctgcatgg 1981 cgccgtgcgg ttcagcaaca accctgccct gtgcaacgtg gagagcatcc agtggcggga 2041 catagtcagc agtgactttc tcagcaacat gtcgatggac ttccagaacc acctgggcag 2101 ctgccaaaag tgtgatccaa gctgtcccaa tgggagctgc tggggtgcag gagaggagaa 2161 ctgccagaaa ctgaccaaaa tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa 2221 gtcccccagt gactgctgcc acaaccagtg tgctgcaggc tgcacaggcc cccgggagag 2281 cgactgcctg gtctgccgca aattccgaga cgaagccacg tgcaaggaca cctgcccccc 2341 actcatgctc tacaacccca ccacgtacca gatggatgtg aaccccgagg gcaaatacag 2401 ctttggtgcc acctgcgtga agaagtgtcc ccgtaattat gtggtgacag atcacggctc 2461 gtgcgtccga gcctgtgggg ccgacagcta tgagatggag gaagacggcg tccgcaagtg 2521 taagaagtgc gaagggcctt gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa 2581 agactcactc tccataaatg ctacgaatat taaacacttc aaaaactgca cctccatcag 2641 tggcgatctc cacatcctgc cggtggcatt taggggtgac tccttcacac atactcctcc 2701 tctggatcca caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct 2761 gattcaggct tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat 2821 acgcggcagg accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac 2881 atccttggga ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa 2941 caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca 3001 gaaaaccaaa attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg 3061 ccatgccttg tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg 3121 ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc 3181 aagggagttt gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc 3241 catgaacatc acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat 3301 tgacggcccc cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct 3361 ggtctggaag tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta 3421 cggatgcact gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat 3481 cgccactggg atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct 3541 cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag 3601 ggagcttgtg gagcctctta cacccagtgg agaagctccc aaccaagctc tcttgaggat 3661 cttgaaggaa actgaattca aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt 3721 gtataaggga ctctggatcc cagaaggtga gaaagttaaa attcccgtcg ctatcaagga 3781 attaagagaa gcaacatctc cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat 3841 ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc atctgcctca cctccaccgt 3901 gcagctcatc acgcagctca tgcccttcgg ctgcctcctg gactatgtcc ggggacacaa 3961 agacaatatt ggctcccagt acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa 4021 ctacttggag gaccgtcgct tggtgcaccg cgacctggca gccaggaacg tactggtgaa 4081 aacaccgcag catgtcaaga tcacagattt tgggctggcc aaactgctgg gtgcggaaga 4141 gaaagaatac catgcagaag gaggcaaagt gcctatcaag tggatggcat tggaatcaat 4201 tttacacaga atctataccc accagagtga tgtctggagc tacggggtga ccgtttggga 4261 gttgatgacc tttggatcca agccatatga cggaatccct gccagcgaga tctcctccat 4321 cctggagaaa ggagaacgcc tccctcagcc acccatatgt accatcgatg tctacatgat 4381 catggtcaag tgctggatga tagacgcaga tagtcgccca aagttccgtg agttgatcat 4441 cgaattctcc aaaatggccc gagaccccca gcgctacctt gtcattcagg gggatgaaag 4501 aatgcatttg ccaagtccta cagactccaa cttctaccgt gccctgatgg atgaagaaga 4561 catggacgac gtggtggatg ccgacgagta cctcatccca cagcagggct tcttcagcag 4621 cccctccacg tcacggactc ccctcctgag ctctctgagt gcaaccagca acaattccac 4681 cgtggcttgc attgatagaa atgggctgca aagctgtccc atcaaggaag acagcttctt 4741 gcagcgatac agctcagacc ccacaggcgc cttgactgag gacagcatag acgacacctt 4801 cctcccagtg cctgaataca taaaccagtc cgttcccaaa aggcccgctg gctctgtgca 4861 gaatcctgtc tatcacaatc agcctctgaa ccccgcgccc agcagagacc cacactacca 4921 ggacccccac agcactgcag tgggcaaccc cgagtatctc aacactgtcc agcccacctg 4981 tgtcaacagc acattcgaca gccctgccca ctgggcccag aaaggcagcc accaaattag 5041 cctggacaac cctgactacc agcaggactt ctttcccaag gaagccaagc caaatggcat 5101 ctttaagggc tccacagctg aaaatgcaga atacctaagg gtcgcgccac aaagcagtga 5161 atttattgga gcatagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc 5221 actaaagggt ttagtgatta aagatagatt agccctatag tgagtcgtat taccacccaa 5281 cttttctata caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc 5341 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg 5401 gttagtaatg agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 5461 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 5521 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 5581 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 5641 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 5701 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 5761 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 5821 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 5881 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 5941 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc 6001 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 6061 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 6121 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 6181 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 6241 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 6301 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 6361 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 6421 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 6481 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 6541 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat 6601 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 6661 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 6721 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 6781 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6841 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6901 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6961 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 7021 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 7081 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 7141 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga 7201 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 7261 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt 7321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 7381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 7441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 7501 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 7561 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa 7621 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 7681 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 7741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 7801 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 7861 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 7921 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 7981 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 8041 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc 8101 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 8161 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 8221 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 8281 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 8341 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 8401 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 8461 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 8521 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 8581 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 8641 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 8701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 8761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 8821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 8881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 8941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 9001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 9061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 9121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 9181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 9241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 9301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 9361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 9421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 9481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 9541 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 9601 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 9661 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 9721 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 9781 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 9841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 9901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat 9961 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 10021 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 10081 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 10141 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 10201 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg 10261 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc 10321 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag 10381 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc 10441 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat 10501 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt 10561 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg 10621 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc 10681 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 10741 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 10801 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 10861 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 10921 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 10981 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 11041 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 11101 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 11161 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 11221 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 11281 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 11341 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat 11401 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 11461 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 11521 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 11581 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 11641 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 11701 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 11761 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 11821 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 11881 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 11941 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 12001 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 12061 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 12121 at //