LOCUS Exported 11606 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of BMPR2 Q6*. ACCESSION . VERSION . KEYWORDS pHAGE-BMPR2-Q6* SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11606) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11606) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11606 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 48..1226 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 279..1217 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1174..1194 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1246..1270 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4391..4415) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4423..4441 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4466..4485) /label=T7 /note="T7 promoter, forward primer" promoter complement(4467..4485) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(4491..4511) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 4565..4606 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4627..5194 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4644..5196 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4788..4805) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5015..5034 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5208..5807 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5208..5227) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5704..5724 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5821..6409 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5874..5894) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6292..6303) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6484..6717 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6836..6854) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6922..7026 /gene="bla" /label=AmpR promoter CDS 7027..7887 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7245..7264) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8058..8646 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8547..8566 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8800..8817 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8909..8928 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9057..9078 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9093..9123 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9131..9147 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9136..9158 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9155..9171 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9155..9171 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9221..9240 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 9432..10065 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10112..10237 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10734..10967 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11152..11196 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 11489..11606 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc 61 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 121 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 241 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc 301 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc 361 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 421 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg 481 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 541 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 601 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 661 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 721 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 781 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 841 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 901 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 961 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 1021 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 1081 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 1141 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 1201 tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa 1261 aaagcaggca ccatgacttc ctcgctgtag cggccctggc gggtgccctg gctaccatgg 1321 accatcctgc tggtcagcac tgcggctgct tcgcagaatc aagaacggct atgtgcgttt 1381 aaagatccgt atcagcaaga ccttgggata ggtgagagta gaatctctca tgaaaatggg 1441 acaatattat gctcgaaagg tagcacctgc tatggccttt gggagaaatc aaaaggggac 1501 ataaatcttg taaaacaagg atgttggtct cacattggag atccccaaga gtgtcactat 1561 gaagaatgtg tagtaactac cactcctccc tcaattcaga atggaacata ccgtttctgc 1621 tgttgtagca cagatttatg taatgtcaac tttactgaga attttccacc tcctgacaca 1681 acaccactca gtccacctca ttcatttaac cgagatgaga caataatcat tgctttggca 1741 tcagtctctg tattagctgt tttgatagtt gccttatgct ttggatacag aatgttgaca 1801 ggagaccgta aacaaggtct tcacagtatg aacatgatgg aggcagcagc atccgaaccc 1861 tctcttgatc tagataatct gaaactgttg gagctgattg gccgaggtcg atatggagca 1921 gtatataaag gctccttgga tgagcgtcca gttgctgtaa aagtgttttc ctttgcaaac 1981 cgtcagaatt ttatcaacga aaagaacatt tacagagtgc ctttgatgga acatgacaac 2041 attgcccgct ttatagttgg agatgagaga gtcactgcag atggacgcat ggaatatttg 2101 cttgtgatgg agtactatcc caatggatct ttatgcaagt atttaagtct ccacacaagt 2161 gactgggtaa gctcttgccg tcttgctcat tctgttacta gaggactggc ttatcttcac 2221 acagaattac cacgaggaga tcattataaa cctgcaattt cccatcgaga tttaaacagc 2281 agaaatgtcc tagtgaaaaa tgatggaacc tgtgttatta gtgactttgg actgtccatg 2341 aggctgactg gaaatagact ggtgcgccca ggggaggaag ataatgcagc cataagcgag 2401 gttggcacta tcagatatat ggcaccagaa gtgctagaag gagctgtgaa cttgagggac 2461 tgtgaatcag ctttgaaaca agtagacatg tatgctcttg gactaatcta ttgggagata 2521 tttatgagat gtacagacct cttcccaggg gaatccgtac cagagtacca gatggctttt 2581 cagacagagg ttggaaacca tcccactttt gaggatatgc aggttctcgt gtctagggaa 2641 aaacagagac ccaagttccc agaagcctgg aaagaaaata gcctggcagt gaggtcactc 2701 aaggagacaa tcgaagactg ttgggaccag gatgcagagg ctcggcttac tgcacagtgt 2761 gctgaggaaa ggatggctga acttatgatg atttgggaaa gaaacaaatc tgtgagccca 2821 acagtcaatc caatgtctac tgctatgcag aatgaacgca acctgtcaca taataggcgt 2881 gtgccaaaaa ttggtcctta tccagattat tcttcctcct catacattga agactctatc 2941 catcatactg acagcatcgt gaagaatatt tcctctgagc attctatgtc cagcacacct 3001 ttgactatag gggaaaaaaa ccgaaattca attaactatg aacgacagca agcacaagct 3061 cgaatcccca gccctgaaac aagtgtcacc agcctctcca ccaacacaac aaccacaaac 3121 accacaggac tcacgccaag tactggcatg actactatat ctgagatgcc atacccagat 3181 gaaacaaatc tgcataccac aaatgttgca cagtcaattg ggccaacccc tgtctgctta 3241 cagctgacag aagaagactt ggaaaccaac aagctagacc caaaagaagt tgataagaac 3301 ctcaaggaaa gctctgatga gaatctcatg gagcactctc ttaaacagtt cagtggccca 3361 gacccactga gcagtactag ttctagcttg ctttacccac tcataaaact tgcagtagaa 3421 gcaactggac agcaggactt cacacagact gcaaatggcc aagcatgttt gattcctgat 3481 gttctgccta ctcagatcta tcctctcccc aagcagcaga accttcccaa gagacctact 3541 agtttgcctt tgaacaccaa aaattcaaca aaagagcccc ggctaaaatt tggcagcaag 3601 cacaaatcaa acttgaaaca agtcgaaact ggagttgcca agatgaatac aatcaatgca 3661 gcagaacctc atgtggtgac agtcaccatg aatggtgtgg caggtagaaa ccacagtgtt 3721 aactcccatg ctgccacaac ccaatatgcc aatgggacag tactatctgg ccaaacaacc 3781 aacatagtga cacatagggc ccaagaaatg ttgcagaatc agtttattgg tgaggacacc 3841 cggctgaata ttaattccag tcctgatgag catgagcctt tactgagacg agagcaacaa 3901 gctggccatg atgaaggtgt tctggatcgt cttgtggaca ggagggaacg gccactagaa 3961 ggtggccgaa ctaattccaa taacaacaac agcaatccat gttcagaaca agatgttctt 4021 gcacagggtg ttccaagcac agcagcagat cctgggccat caaagcccag aagagcacag 4081 aggcctaatt ctctggatct ttcagccaca aatgtcctgg atggcagcag tatacagata 4141 ggtgagtcaa cacaagatgg caaatcagga tcaggtgaaa agatcaagaa acgtgtgaaa 4201 actccctatt ctcttaagcg gtggcgcccc tccacctggg tcatctccac tgaatcgctg 4261 gactgtgaag tcaacaataa tggcagtaac agggcagttc attccaaatc cagcactgct 4321 gtttaccttg cagaaggagg cactgctaca accatggtgt ctaaagatat aggaatgaac 4381 tgtctgtaga acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag 4441 ggtgtaaatg tttgagatgt atattcccta tagtgagtcg tattaccacc caacttttct 4501 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt 4561 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta 4621 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg 4681 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg 4741 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 4801 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 4861 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 4921 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 4981 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 5041 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 5101 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg 5161 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca 5221 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 5281 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 5341 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 5401 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 5461 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 5521 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 5581 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 5641 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 5701 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 5761 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct 5821 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5881 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5941 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6001 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6061 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6121 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6181 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 6241 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6301 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 6361 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt 6421 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6481 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 6541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6721 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6781 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc 6841 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 6901 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 6961 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 7021 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 7081 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 7141 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 7201 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 7261 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 7321 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 7381 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 7441 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 7501 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 7561 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 7621 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 7681 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 7741 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 7801 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 7861 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 7921 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 7981 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 8041 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 8101 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 8161 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 8221 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 8281 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 8341 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 8401 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 8461 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 8521 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 8581 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 8641 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 8701 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 8761 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 8821 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 8881 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 8941 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 9001 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 9061 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 9121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg 9181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 9241 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa 9301 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc 9361 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca 9421 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg 9481 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt 9541 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt 9601 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat 9661 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca 9721 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa 9781 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg 9841 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag 9901 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 9961 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 10021 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 10081 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 10141 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 10201 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 10261 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 10321 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 10381 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 10441 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 10501 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 10561 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac 10621 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 10681 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 10741 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 10801 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 10861 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 10921 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 10981 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 11041 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 11101 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 11161 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 11221 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 11281 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 11341 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 11401 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga 11461 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca 11521 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa 11581 aacaaattac aaaaattcaa aatttt //