LOCUS Exported 9685 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of TGFB1 P10L. ACCESSION . VERSION . KEYWORDS pHAGE-TGFB1-P10L SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9685) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9685) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9685 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4622..4646) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4699..4740 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4761..5328 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4778..5330 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4922..4939) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5149..5168 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5342..6061 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5342..6058 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5387..5408) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5648..5667) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5995..6016 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6075..6663 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6128..6148) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6546..6557) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6738..6971 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7090..7108) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7176..7280 /gene="bla" /label=AmpR promoter CDS 7281..8141 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7499..7518) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8312..8900 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8801..8820 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9054..9071 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9163..9182 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9311..9332 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9347..9377 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9385..9401 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9390..9412 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9409..9425 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9409..9425 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9475..9494 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg ccgccctccg ggctgcggct gctgctgctg 3481 ctgctaccgc tgctgtggct actggtgctg acgcctggcc ggccggccgc gggactatcc 3541 acctgcaaga ctatcgacat ggagctggtg aagcggaagc gcatcgaggc catccgcggc 3601 cagatcctgt ccaagctgcg gctcgccagc cccccgagcc agggggaggt gccgcccggc 3661 ccgctgcccg aggccgtgct cgccctgtac aacagcaccc gcgaccgggt ggccggggag 3721 agtgcagaac cggagcccga gcctgaggcc gactactacg ccaaggaggt cacccgcgtg 3781 ctaatggtgg aaacccacaa cgaaatctat gacaagttca agcagagtac acacagcata 3841 tatatgttct tcaacacatc agagctccga gaagcggtac ctgaacccgt gttgctctcc 3901 cgggcagagc tgcgtctgct gaggctcaag ttaaaagtgg agcagcacgt ggagctgtac 3961 cagaaataca gcaacaattc ctggcgatac ctcagcaacc ggctgctggc acccagcgac 4021 tcgccagagt ggttatcttt tgatgtcacc ggagttgtgc ggcagtggtt gagccgtgga 4081 ggggaaattg agggctttcg ccttagcgcc cactgctcct gtgacagcag ggataacaca 4141 ctgcaagtgg acatcaacgg gttcactacc ggccgccgag gtgacctggc caccattcat 4201 ggcatgaacc ggcctttcct gcttctcatg gccaccccgc tggagagggc ccagcatctg 4261 caaagctccc ggcaccgccg agccctggac accaactatt gcttcagctc cacggagaag 4321 aactgctgcg tgcggcagct gtacattgac ttccgcaagg acctcggctg gaagtggatc 4381 cacgagccca agggctacca tgccaacttc tgcctcgggc cctgccccta catttggagc 4441 ctggacacgc agtacagcaa ggtcctggcc ctgtacaacc agcataaccc gggcgcctcg 4501 gcggcgccgt gctgcgtgcc gcaggcgctg gagccgctgc ccatcgtgta ctacgtgggc 4561 cgcaagccca aggtggagca gctgtccaac atgatcgtgc gctcctgcaa gtgcagctag 4621 aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta 4681 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg 4741 cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg 4801 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 4861 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 4921 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 4981 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 5041 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 5101 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 5161 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat 5221 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc 5281 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca 5341 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 5401 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 5461 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 5521 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 5581 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 5641 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 5701 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 5761 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 5821 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 5881 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 5941 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 6001 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 6061 aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 6121 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 6181 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 6241 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 6301 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 6361 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 6421 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 6481 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 6541 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6601 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 6661 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 6721 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 6781 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6841 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6901 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6961 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 7021 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa 7081 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 7141 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 7201 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 7261 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 7321 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 7381 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 7441 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 7501 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 7561 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 7621 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 7681 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 7741 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 7801 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 7861 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 7921 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 7981 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 8041 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 8101 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 8161 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 8221 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 8281 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 8341 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 8401 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 8461 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 8521 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 8581 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 8641 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 8701 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 8761 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 8821 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 8881 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 8941 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 9001 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 9061 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 9121 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga 9181 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 9241 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 9301 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 9361 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 9421 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt 9481 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc 9541 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc 9601 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt 9661 aaatatgtac tacaaactta gtagt //