LOCUS Exported 10791 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of BRAF T599Ifs*13. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-T599Ifs*13 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10791) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10791) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10791 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 20..44 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 45..54 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(2350..2374) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 2382..2400 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2425..2444) /label=T7 /note="T7 promoter, forward primer" promoter complement(2426..2444) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(2450..2470) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 2524..2565 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2586..3153 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 2603..3155 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2747..2764) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2974..2993 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3167..3766 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3167..3186) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3663..3683 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 3780..4368 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3833..3853) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4251..4262) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4443..4676 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(4795..4813) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4881..4985 /gene="bla" /label=AmpR promoter CDS 4986..5846 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5204..5223) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6017..6605 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6506..6525 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6759..6776 /label=L4440 /note="L4440 vector, forward primer" primer_bind 6868..6887 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 7016..7037 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7052..7082 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7090..7106 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7095..7117 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7114..7130 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7114..7130 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7180..7199 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 7391..8024 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 8071..8196 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8693..8926 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9111..9155 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9448..9565 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 9613..10791 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 9844..10782 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 10739..10759 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cggcgctgag 61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc 121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga 181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt 241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata 301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa 361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc 421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc 481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca 541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact 601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa 661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga 721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt 781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg 841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca 901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc 961 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc 1021 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc 1081 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc 1141 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga 1201 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc 1261 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga 1321 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga 1381 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattggatc 1441 tggatcattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt 1501 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact 1561 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact 1621 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga 1681 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga 1741 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca 1801 tgaagacctc acagtaaaaa taggtgattt tggtctagct atacgtgaaa tctcgatgga 1861 gtgggtccca tcagtttgaa cagttgtctg gatccatttt gtggatggca ccagaagtca 1921 tcagaatgca agataaaaat ccatacagct ttcagtcaga tgtatatgca tttggaattg 1981 ttctgtatga attgatgact ggacagttac cttattcaaa catcaacaac agggaccaga 2041 taatttttat ggtgggacga ggatacctgt ctccagatct cagtaaggta cggagtaact 2101 gtccaaaagc catgaagaga ttaatggcag agtgcctcaa aaagaaaaga gatgagagac 2161 cactctttcc ccaaattctc gcctctattg agctgctggc ccgctcattg ccaaaaattc 2221 accgcagtgc atcagaaccc tccttgaatc gggctggttt ccaaacagag gattttagtc 2281 tatatgcttg tgcttctcca aaaacaccca tccaggcagg gggatatggt gcgtttcctg 2341 tccactagaa cccagctttc ttgtacaaag tggtgatatc caattaaccc tcactaaagg 2401 ttgtgatagt agttagatat ttgtccctat agtgagtcgt attaccaccc aacttttcta 2461 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc 2521 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa 2581 tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt 2641 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg 2701 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga 2761 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa 2821 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc 2881 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac 2941 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag 3001 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc 3061 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg 3121 gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatga ccgagtacaa 3181 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 3241 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 3301 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 3361 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 3421 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 3481 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 3541 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 3601 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 3661 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 3721 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaatcg atagatccta 3781 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 3841 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 3901 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 3961 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4021 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4081 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4141 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 4201 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 4261 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 4321 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt 4381 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 4441 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 4501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 4561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 4621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 4681 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 4741 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg 4801 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 4861 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 4921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 4981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 5041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 5101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 5161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 5221 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 5281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 5341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 5401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 5461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 5521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 5581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 5641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 5701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 5761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 5821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 5881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 5941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 6001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 6061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 6121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 6181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 6241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 6301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 6361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 6421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 6481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 6541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 6601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 6661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 6721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 6781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 6841 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 6961 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 7021 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 7081 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga 7141 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7201 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac 7261 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct 7321 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa 7381 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg 7441 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 7501 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 7561 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 7621 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 7681 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 7741 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 7801 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 7861 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 7921 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7981 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg 8041 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa 8101 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc 8161 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga 8221 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat 8281 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca 8341 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa 8401 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag 8461 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc 8521 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca 8581 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 8641 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 8701 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 8761 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 8821 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 8881 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 8941 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 9001 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 9061 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 9121 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 9181 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 9241 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 9301 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 9361 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa 9421 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag 9481 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa 9541 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg 9601 gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 9661 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta 9721 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 9781 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 9841 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc 9901 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt 9961 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga 10021 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc 10081 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg 10141 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg 10201 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg 10261 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct 10321 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc 10381 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc 10441 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa 10501 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc 10561 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg 10621 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg 10681 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc 10741 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg a //