LOCUS       Exported               10791 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of BRAF T599Ffs*13.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-BRAF-T599Ffs*13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1224..1233
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(3529..3553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3561..3579
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3604..3623)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3605..3623)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3629..3649)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3703..3744
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3765..4332
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3782..4334
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3926..3943)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4153..4172
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4346..4945
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4346..4365)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4842..4862
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4959..5547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5012..5032)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5430..5441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5622..5855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5974..5992)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6060..6164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6165..7025
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6383..6402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7196..7784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7685..7704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7938..7955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8047..8066
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    8195..8216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8231..8261
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8269..8285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8274..8296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8293..8309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8293..8309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8359..8378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8570..9203
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9250..9375
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9872..10105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10290..10334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10627..10744
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatggc ggcgctgagc ggtggcggtg gtggcggcgc
     1261 ggagccgggc caggctctgt tcaacgggga catggagccc gaggccggcg ccggcgccgg
     1321 cgccgcggcc tcttcggctg cggaccctgc cattccggag gaggtgtgga atatcaaaca
     1381 aatgattaag ttgacacagg aacatataga ggccctattg gacaaatttg gtggggagca
     1441 taatccacca tcaatatatc tggaggccta tgaagaatac accagcaagc tagatgcact
     1501 ccaacaaaga gaacaacagt tattggaatc tctggggaac ggaactgatt tttctgtttc
     1561 tagctctgca tcaatggata ccgttacatc ttcttcctct tctagccttt cagtgctacc
     1621 ttcatctctt tcagtttttc aaaatcccac agatgtggca cggagcaacc ccaagtcacc
     1681 acaaaaacct atcgttagag tcttcctgcc caacaaacag aggacagtgg tacctgcaag
     1741 gtgtggagtt acagtccgag acagtctaaa gaaagcactg atgatgagag gtctaatccc
     1801 agagtgctgt gctgtttaca gaattcagga tggagagaag aaaccaattg gttgggacac
     1861 tgatatttcc tggcttactg gagaagaatt gcatgtggaa gtgttggaga atgttccact
     1921 tacaacacac aactttgtac gaaaaacgtt tttcacctta gcattttgtg acttttgtcg
     1981 aaagctgctt ttccagggtt tccgctgtca aacatgtggt tataaatttc accagcgttg
     2041 tagtacagaa gttccactga tgtgtgttaa ttatgaccaa cttgatttgc tgtttgtctc
     2101 caagttcttt gaacaccacc caataccaca ggaagaggcg tccttagcag agactgccct
     2161 aacatctgga tcatcccctt ccgcacccgc ctcggactct attgggcccc aaattctcac
     2221 cagtccgtct ccttcaaaat ccattccaat tccacagccc ttccgaccag cagatgaaga
     2281 tcatcgaaat caatttgggc aacgagaccg atcctcatca gctcccaatg tgcatataaa
     2341 cacaatagaa cctgtcaata ttgatgactt gattagagac caaggatttc gtggtgatgg
     2401 aggatcaacc acaggtttgt ctgctacccc ccctgcctca ttacctggct cactaactaa
     2461 cgtgaaagcc ttacagaaat ctccaggacc tcagcgagaa aggaagtcat cttcatcctc
     2521 agaagacagg aatcgaatga aaacacttgg tagacgggac tcgagtgatg attgggagat
     2581 tcctgatggg cagattacag tgggacaaag aattggatct ggatcatttg gaacagtcta
     2641 caagggaaag tggcatggtg atgtggcagt gaaaatgttg aatgtgacag cacctacacc
     2701 tcagcagtta caagccttca aaaatgaagt aggagtactc aggaaaacac gacatgtgaa
     2761 tatcctactc ttcatgggct attccacaaa gccacaactg gctattgtta cccagtggtg
     2821 tgagggctcc agcttgtatc accatctcca tatcattgag accaaatttg agatgatcaa
     2881 acttatagat attgcacgac agactgcaca gggcatggat tacttacacg ccaagtcaat
     2941 catccacaga gacctcaaga gtaataatat atttcttcat gaagacctca cagtaaaaat
     3001 aggtgatttt ggtctagcgt tcagtgaaat ctcgatggag tgggtcccat cagtttgaac
     3061 agttgtctgg atccattttg tggatggcac cagaagtcat cagaatgcaa gataaaaatc
     3121 catacagctt tcagtcagat gtatatgcat ttggaattgt tctgtatgaa ttgatgactg
     3181 gacagttacc ttattcaaac atcaacaaca gggaccagat aatttttatg gtgggacgag
     3241 gatacctgtc tccagatctc agtaaggtac ggagtaactg tccaaaagcc atgaagagat
     3301 taatggcaga gtgcctcaaa aagaaaagag atgagagacc actctttccc caaattctcg
     3361 cctctattga gctgctggcc cgctcattgc caaaaattca ccgcagtgca tcagaaccct
     3421 ccttgaatcg ggctggtttc caaacagagg attttagtct atatgcttgt gcttctccaa
     3481 aaacacccat ccaggcaggg ggatatggtg cgtttcctgt ccactagaac ccagctttct
     3541 tgtacaaagt ggtgatatcc aattaaccct cactaaaggt tgtgatagta gttagatatt
     3601 tgtccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt tgatatccag
     3661 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     3721 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc ccccccccnt
     3781 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3841 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3901 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3961 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     4021 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4081 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     4141 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     4201 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     4261 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     4321 acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc gcctcgccac
     4381 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4441 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4501 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4561 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4621 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4681 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4741 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4801 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4861 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4921 catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg gattacaaaa
     4981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5521 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     5581 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5641 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5821 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     5881 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     5941 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     6001 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     6421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
     8041 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8101 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
     8161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8281 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
     8341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     8401 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     8461 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     8521 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     8581 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8641 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     8701 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     8761 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     8821 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     8881 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     8941 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     9001 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9181 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9241 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9301 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9361 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9421 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9481 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9541 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9601 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9661 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     9721 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9781 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9841 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9901 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9961 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10021 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10081 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10141 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10201 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10261 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10321 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10381 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10441 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10501 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    10561 ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg cagtattcat
    10621 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    10681 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    10741 ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtcgtg a
//
