LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR G719C. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-G719C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 239..356 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 404..1582 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 635..1573 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1530..1550 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1602..1626 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1629..5261 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLCSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5263..5287) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5295..5313 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5338..5357) /label=T7 /note="T7 promoter, forward primer" promoter complement(5339..5357) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5363..5383) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5437..5478 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5499..6066 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5516..6068 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5660..5677) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5887..5906 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6080..6679 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6080..6099) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6576..6596 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6693..7281 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6746..6766) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7164..7175) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7356..7589 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7708..7726) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7794..7898 /gene="bla" /label=AmpR promoter CDS 7899..8759 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8117..8136) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8930..9518 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9419..9438 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9672..9689 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9781..9800 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9929..9950 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9965..9995 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10003..10019 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10008..10030 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10027..10043 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10027..10043 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10093..10112 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10304..10937 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10984..11109 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11606..11839 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12024..12068 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga 61 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc 121 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga 181 ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc atccacaatt 241 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 301 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg 361 tttattacag ggacagcaga gatccagttt ggactagtcg tgaggctccg gtgcccgtca 421 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 481 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 541 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 601 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 661 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 721 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 781 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 841 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 901 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 961 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 1021 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 1081 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 1141 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 1201 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 1261 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 1321 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 1381 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 1441 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 1501 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 1561 tcttccattt caggtgtcgt gaagcggccc tgcagatatc aacaagtttg tacaaaaaag 1621 caggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc 1681 tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc 1741 tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact 1801 gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct 1861 tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc 1921 gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg 1981 ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga 2041 gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca 2101 acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga 2161 tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga 2221 gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc 2281 agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg 2341 caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag 2401 ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg 2461 atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta 2521 attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga 2581 tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta 2641 acggaatagg tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac 2701 acttcaaaaa ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg 2761 gtgactcctt cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg 2821 taaaggaaat cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc 2881 atgcctttga gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc 2941 ttgcagtcgt cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg 3001 atggagatgt gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga 3061 aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca 3121 gctgcaaggc cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc 3181 cggagcccag ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca 3241 agtgcaacct tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt 3301 gccacccaga gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca 3361 actgtatcca gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag 3421 gagtcatggg agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc 3481 acctgtgcca tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa 3541 cgaatgggcc taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc 3601 tggtggtggc cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca 3661 cgctgcggag gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag 3721 ctcccaacca agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc 3781 tgtgctccgg tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag 3841 ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg 3901 aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc 3961 tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc ttcggctgcc 4021 tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt 4081 gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc 4141 tggcagccag gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttgggc 4201 tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta 4261 tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct 4321 ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa 4381 tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca 4441 tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc 4501 gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct 4561 accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct 4621 accgtgccct gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca 4681 tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc 4741 tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct 4801 gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga 4861 ctgaggacag catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc 4921 ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg 4981 cgcccagcag agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt 5041 atctcaacac tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg 5101 cccagaaagg cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc 5161 ccaaggaagc caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc 5221 taagggtcgc gccacaaagc agtgaattta ttggagcata gaacccagct ttcttgtaca 5281 aagtggtgat atccaattaa ccctcactaa aggtgaaatg tgtatttgta tgtttagccc 5341 tatagtgagt cgtattacca cccaactttt ctatacaaag tggttgatat ccagcacagt 5401 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc 5461 ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc ccctaacgtt 5521 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc 5581 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc 5641 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag 5701 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg 5761 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat 5821 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga 5881 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc 5941 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg 6001 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga 6061 tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 6121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 6181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 6241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 6301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 6361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 6421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 6481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 6541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 6601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 6661 ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg 6721 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 6781 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 6841 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 6901 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 6961 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 7021 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 7081 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 7141 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 7201 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 7261 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt 7321 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 7381 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 7441 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7501 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7561 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7621 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag 7681 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 7741 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 7801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 7861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 7921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 7981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 8041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 8101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 8161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 8221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 8281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 8341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 8401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 8461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 8521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 8581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 8641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 8701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 8761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 8821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 8881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 8941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 9001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 9061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 9121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 9181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 9241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 9301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 9361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 9421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 9481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 9541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 9601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 9661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 9721 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt 9781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 9841 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact 9901 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 9961 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 10021 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac 10081 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 10141 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 10201 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 10261 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac 10321 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 10381 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 10441 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 10501 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 10561 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 10621 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 10681 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 10741 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 10801 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 10861 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 10921 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag 10981 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg 11041 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc 11101 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg 11161 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa 11221 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga 11281 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta 11341 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac 11401 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga 11461 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 11521 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 11581 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 11641 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 11701 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 11761 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 11821 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 11881 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 11941 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 12001 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 12061 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 12121 tg //