LOCUS Exported 11699 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of XPO1 E571K. ACCESSION . VERSION . KEYWORDS pHAGE-XPO1-E571K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11699) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11699) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11699 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6691..6709 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6734..6753) /label=T7 /note="T7 promoter, forward primer" promoter complement(6735..6753) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6759..6779) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6833..6874 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6895..7462 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6912..7464 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7056..7073) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7283..7302 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7476..8075 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7476..7495) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7972..7992 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8089..8677 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8142..8162) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8560..8571) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8752..8985 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9104..9122) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9190..9294 /gene="bla" /label=AmpR promoter CDS 9295..10155 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9513..9532) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10326..10914 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10815..10834 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11068..11085 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11177..11196 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11325..11346 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11361..11391 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11399..11415 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11404..11426 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11423..11439 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11423..11439 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11489..11508 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatgcca gcaattatga caatgttagc agaccatgca 3481 gctcgtcagc tgcttgattt cagccaaaaa ctggatatca acttattaga taatgtggtg 3541 aattgcttat accatggaga aggagcccag caaagaatgg ctcaagaagt actgacacat 3601 ttaaaggagc atcctgatgc ttggacaaga gtcgacacaa ttttggaatt ttctcagaat 3661 atgaatacga aatactatgg actacaaatt ttggaaaatg tgataaaaac aaggtggaag 3721 attcttccaa ggaaccagtg cgaaggaata aaaaaatacg ttgttggcct cattatcaag 3781 acgtcatctg acccaacttg tgtagagaaa gaaaaggtgt atatcggaaa attaaatatg 3841 atccttgttc agatactgaa acaagaatgg cccaaacatt ggccaacttt tatcagtgat 3901 attgttggag caagtaggac cagcgaaagt ctctgtcaaa ataatatggt gattcttaaa 3961 ctcttgagtg aagaagtatt tgatttctct agtggacaga taacccaagt caaatctaag 4021 catttaaaag acagcatgtg caatgaattc tcacagatat ttcaactgtg tcagtttgta 4081 atggaaaatt ctcaaaatgc tccacttgta catgcaacct tggaaacatt gctcagattt 4141 ctgaactgga ttcccctggg atatattttt gagaccaaat taatcagcac attgatttat 4201 aagttcctga atgttccaat gtttcgaaat gtctctctga agtgcctcac tgagattgct 4261 ggtgtgagtg taagccaata tgaagaacaa tttgtaacac tatttactct gacaatgatg 4321 caactaaagc agatgcttcc tttaaatacc aatattcgac ttgcgtactc aaatggaaaa 4381 gatgatgaac agaacttcat tcaaaatctc agtttgtttc tctgcacctt tcttaaggaa 4441 catgatcaac ttatagaaaa aagattaaat ctcagggaaa ctcttatgga ggcccttcat 4501 tatatgttgt tggtatctga agtagaagaa actgaaatct ttaaaatttg tcttgaatac 4561 tggaatcatt tggctgctga actctataga gagagtccat tctctacatc tgcctctccg 4621 ttgctttctg gaagtcaaca ttttgatgtt cctcccagga gacagctata tttgcccatg 4681 ttattcaagg tccgtttatt aatggttagt cgaatggcta aaccagagga agtattggtt 4741 gtagagaatg atcaaggaga agttgtgaga gaattcatga aggatacaga ttccataaat 4801 ttgtataaga atatgaggga aacattggtt tatcttactc atctggatta tgtagataca 4861 gaaagaataa tgacagagaa gcttcacaat caagtgaatg gtacagagtg gtcatggaaa 4921 aatttgaata cattgtgttg ggcaataggc tccattagtg gagcaatgca tgaagaggac 4981 gaaaaacgat ttcttgttac tgttataaag gatctattag gattatgtga acagaaaaga 5041 ggcaaagata ataaagctat tattgcatca aatatcatgt acatagtagg tcaataccca 5101 cgttttttga gagctcactg gaaatttctg aagactgtag ttaacaagct gttcaaattc 5161 atgcatgaga cccatgatgg agtccaggat atggcttgtg atactttcat taaaatagcc 5221 caaaaatgcc gcaggcattt cgttcaggtt caggttggag aagtgatgcc atttattgat 5281 gaaattttga acaacattaa cactattatt tgtgatcttc agcctcaaca ggttcatacg 5341 ttttatgaag ctgtggggta catgattggt gcacaaacag atcaaacagt acaagaacac 5401 ttgatagaaa agtacatgtt actccctaat caagtgtggg atagtataat ccagcaggca 5461 accaaaaatg tggatatact gaaagatcct gaaacagtca agcagcttgg tagcattttg 5521 aaaacaaatg tgagagcctg caaagctgtt ggacacccct ttgtaattca gcttggaaga 5581 atttatttag atatgcttaa tgtatacaag tgcctcagtg aaaatatttc tgcagctatc 5641 caagctaatg gtgaaatggt tacaaagcaa ccattgatta gaagtatgcg aactgtaaaa 5701 agggaaactt taaagttaat atctggttgg gtgagccgat ccaatgatcc acagatggtc 5761 gctgaaaatt ttgttccccc tctgttggat gcagttctca ttgattatca gagaaatgtc 5821 ccagctgcta gagaaccaga agtgcttagt actatggcca taattgtcaa caagttaggg 5881 ggacatataa cagctgaaat acctcaaata tttgatgctg tttttgaatg cacattgaat 5941 atgataaata aggactttga agaatatcct gaacatagaa cgaacttttt cttactactt 6001 caggctgtca attctcattg tttcccagca ttccttgcta ttccacctac acagtttaaa 6061 cttgttttgg attccatcat ttgggctttc aaacatacta tgaggaatgt cgcagatacg 6121 ggcttacaga tactttttac actcttacaa aatgttgcac aagaagaagc tgcagctcag 6181 agtttttatc aaacttattt ttgtgatatt ctccagcata tcttttctgt tgtgacagac 6241 acttcacata ctgctggttt aacaatgcat gcatcaattc ttgcatatat gtttaatttg 6301 gttgaagaag gaaaaataag tacatcatta aatcctggaa atccagttaa caaccaaatc 6361 tttcttcagg aatatgtggc taatctcctt aagtcggcct tccctcacct acaagatgct 6421 caagtaaagc tctttgtgac agggcttttc agcttaaatc aagatattcc tgctttcaag 6481 gaacatttaa gagatttcct agttcaaata aaggaatttg caggtgaaga cacttctgat 6541 ttgtttttgg aagagagaga aatagcccta cggcaggctg atgaagagaa acataaacgt 6601 caaatgtctg tccctggcat ctttaatcca catgagattc cagaagaaat gtgtgattac 6661 ccaacattct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt gatatagtag 6721 tgaagaaata agtccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt 6781 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc 6841 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc 6901 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta 6961 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc 7021 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct 7081 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt 7141 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa 7201 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg 7261 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga 7321 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac 7381 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt 7441 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc 7501 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 7561 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 7621 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 7681 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 7741 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 7801 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 7861 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 7921 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 7981 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 8041 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg 8101 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 8161 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 8221 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 8281 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 8341 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 8401 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 8461 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 8521 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 8581 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 8641 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga 8701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 8761 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 8821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 8881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 8941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 9001 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg 9061 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta 9121 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 9181 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 9241 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 9301 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 9361 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 9421 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 9481 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt 9541 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 9601 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 9661 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 9721 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 9781 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 9841 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 9901 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 9961 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 10021 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 10081 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 10141 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 10201 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 10261 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 10321 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 10381 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 10441 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 10501 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 10561 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 10621 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 10681 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 10741 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 10801 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 10861 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 10921 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 10981 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 11041 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 11101 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg 11161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 11221 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac 11281 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 11341 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 11401 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat 11461 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 11521 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 11581 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 11641 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt //