LOCUS Exported 12257 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of ERBB2 E321G. ACCESSION . VERSION . KEYWORDS pHAGE-ERBB2-E321G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12257) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12257) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12257 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7217..7241) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7249..7267 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7292..7311) /label=T7 /note="T7 promoter, forward primer" promoter complement(7293..7311) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(7317..7337) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 7391..7432 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7453..8020 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7470..8022 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7614..7631) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7841..7860 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8034..8633 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8034..8053) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8530..8550 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8647..9235 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8700..8720) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9118..9129) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9310..9543 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9662..9680) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9748..9852 /gene="bla" /label=AmpR promoter CDS 9853..10713 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10071..10090) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10884..11472 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11373..11392 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11626..11643 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11735..11754 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11883..11904 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11919..11949 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11957..11973 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11962..11984 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11981..11997 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11981..11997 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12047..12066 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gagctggcgg ccttgtgccg ctgggggctc 3481 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg 3541 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag 3601 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg 3661 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg 3721 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac 3781 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg 3841 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga 3901 ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt gtggaaggac 3961 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc 4021 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat 4081 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg 4141 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac 4201 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg 4261 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc 4321 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc 4381 accctcgtct gccccctgca caaccaaggg gtgacagcag aggatggaac acagcggtgt 4441 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga 4501 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt 4561 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg 4621 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac 4681 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc 4741 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc 4801 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac 4861 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa 4921 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc 4981 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc 5041 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc 5101 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat 5161 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag 5221 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg 5281 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc 5341 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg 5401 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt 5461 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg 5521 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag 5581 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt 5641 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc 5701 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca 5761 tacgtgatgg ctggtgtggg ctccccatat gtctcccgcc ttctgggcat ctgcctgaca 5821 tccacggtgc agctggtgac acagcttatg ccctatggct gcctcttaga ccatgtccgg 5881 gaaaaccgcg gacgcctggg ctcccaggac ctgctgaact ggtgtatgca gattgccaag 5941 gggatgagct acctggagga tgtgcggctc gtacacaggg acttggccgc tcggaacgtg 6001 ctggtcaaga gtcccaacca tgtcaaaatt acagacttcg ggctggctcg gctgctggac 6061 attgacgaga cagagtacca tgcagatggg ggcaaggtgc ccatcaagtg gatggcgctg 6121 gagtccattc tccgccggcg gttcacccac cagagtgatg tgtggagtta tggtgtgact 6181 gtgtgggagc tgatgacttt tggggccaaa ccttacgatg ggatcccagc ccgggagatc 6241 cctgacctgc tggaaaaggg ggagcggctg ccccagcccc ccatctgcac cattgatgtc 6301 tacatgatca tggtcaaatg ttggatgatt gactctgaat gtcggccaag attccgggag 6361 ttggtgtctg aattctcccg catggccagg gacccccagc gctttgtggt catccagaat 6421 gaggacttgg gcccagccag tcccttggac agcaccttct accgctcact gctggaggac 6481 gatgacatgg gggacctggt ggatgctgag gagtatctgg taccccagca gggcttcttc 6541 tgtccagacc ctgccccggg cgctgggggc atggtccacc acaggcaccg cagctcatct 6601 accaggagtg gcggtgggga cctgacacta gggctggagc cctctgaaga ggaggccccc 6661 aggtctccac tggcaccctc cgaaggggct ggctccgatg tatttgatgg tgacctggga 6721 atgggggcag ccaaggggct gcaaagcctc cccacacatg accccagccc tctacagcgg 6781 tacagtgagg accccacagt acccctgccc tctgagactg atggctacgt tgcccccctg 6841 acctgcagcc cccagcctga atatgtgaac cagccagatg ttcggcccca gcccccttcg 6901 ccccgagagg gccctctgcc tgctgcccga cctgctggtg ccactctgga aaggcccaag 6961 actctctccc cagggaagaa tggggtcgtc aaagacgttt ttgcctttgg gggtgccgtg 7021 gagaaccccg agtacttgac accccaggga ggagctgccc ctcagcccca ccctcctcct 7081 gccttcagcc cagccttcga caacctctat tactgggacc aggacccacc agagcggggg 7141 gctccaccca gcaccttcaa agggacacct acggcagaga acccagagta cctgggtctg 7201 gacgtgccag tgtagaaccc agctttcttg tacaaagtgg tgatatccaa ttaaccctca 7261 ctaaagggtt agttatgatg aatattgtgt accctatagt gagtcgtatt accacccaac 7321 ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc 7381 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg 7441 ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 7501 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 7561 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 7621 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 7681 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 7741 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 7801 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 7861 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 7921 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 7981 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg 8041 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 8101 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 8161 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 8221 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 8281 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 8341 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 8401 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 8461 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 8521 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 8581 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata 8641 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 8701 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 8761 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 8821 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 8881 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 8941 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 9001 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 9061 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 9121 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 9181 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag 9241 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 9301 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 9361 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 9421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 9481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 9541 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 9601 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag 9661 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 9721 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 9781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 9841 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 9901 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 9961 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 10021 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 10081 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct 10141 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 10201 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 10261 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 10321 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 10381 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 10441 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 10501 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 10561 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 10621 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 10681 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 10741 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 10801 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 10861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 10921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 10981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 11041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 11101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 11161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 11221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 11281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 11341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 11401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 11461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 11521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 11581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 11641 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 11701 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 11761 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 11821 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 11881 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 11941 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg 12001 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 12061 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 12121 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 12181 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 12241 actacaaact tagtagt //