LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR G983R. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-G983R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 206..323 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 371..1549 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 602..1540 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1497..1517 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1569..1593 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1596..5228 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQRDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5230..5254) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5262..5280 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5305..5324) /label=T7 /note="T7 promoter, forward primer" promoter complement(5306..5324) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5330..5350) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5404..5445 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5466..6033 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5483..6035 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5627..5644) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5854..5873 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6047..6646 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6047..6066) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6543..6563 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6660..7248 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6713..6733) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7131..7142) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7323..7556 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7675..7693) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7761..7865 /gene="bla" /label=AmpR promoter CDS 7866..8726 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8084..8103) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8897..9485 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9386..9405 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9639..9656 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9748..9767 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9896..9917 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9932..9962 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9970..9986 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9975..9997 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9994..10010 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9994..10010 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10060..10079 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10271..10904 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10951..11076 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11573..11806 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11991..12035 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 61 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 121 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt 181 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg 241 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac 301 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga 361 ctagtcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 421 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa 481 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 541 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 601 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt 661 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg 721 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt 781 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg 841 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct 901 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt 961 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg 1021 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc 1081 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg 1141 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc 1201 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag 1261 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag 1321 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 1381 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 1441 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 1501 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc 1561 agatatcaac aagtttgtac aaaaaagcag gcaccatgcg accctccggg acggccgggg 1621 cagcgctcct ggcgctgctg gctgcgctct gcccggcgag tcgggctctg gaggaaaaga 1681 aagtttgcca aggcacgagt aacaagctca cgcagttggg cacttttgaa gatcattttc 1741 tcagcctcca gaggatgttc aataactgtg aggtggtcct tgggaatttg gaaattacct 1801 atgtgcagag gaattatgat ctttccttct taaagaccat ccaggaggtg gctggttatg 1861 tcctcattgc cctcaacaca gtggagcgaa ttcctttgga aaacctgcag atcatcagag 1921 gaaatatgta ctacgaaaat tcctatgcct tagcagtctt atctaactat gatgcaaata 1981 aaaccggact gaaggagctg cccatgagaa atttacagga aatcctgcat ggcgccgtgc 2041 ggttcagcaa caaccctgcc ctgtgcaacg tggagagcat ccagtggcgg gacatagtca 2101 gcagtgactt tctcagcaac atgtcgatgg acttccagaa ccacctgggc agctgccaaa 2161 agtgtgatcc aagctgtccc aatgggagct gctggggtgc aggagaggag aactgccaga 2221 aactgaccaa aatcatctgt gcccagcagt gctccgggcg ctgccgtggc aagtccccca 2281 gtgactgctg ccacaaccag tgtgctgcag gctgcacagg cccccgggag agcgactgcc 2341 tggtctgccg caaattccga gacgaagcca cgtgcaagga cacctgcccc ccactcatgc 2401 tctacaaccc caccacgtac cagatggatg tgaaccccga gggcaaatac agctttggtg 2461 ccacctgcgt gaagaagtgt ccccgtaatt atgtggtgac agatcacggc tcgtgcgtcc 2521 gagcctgtgg ggccgacagc tatgagatgg aggaagacgg cgtccgcaag tgtaagaagt 2581 gcgaagggcc ttgccgcaaa gtgtgtaacg gaataggtat tggtgaattt aaagactcac 2641 tctccataaa tgctacgaat attaaacact tcaaaaactg cacctccatc agtggcgatc 2701 tccacatcct gccggtggca tttaggggtg actccttcac acatactcct cctctggatc 2761 cacaggaact ggatattctg aaaaccgtaa aggaaatcac agggtttttg ctgattcagg 2821 cttggcctga aaacaggacg gacctccatg cctttgagaa cctagaaatc atacgcggca 2881 ggaccaagca acatggtcag ttttctcttg cagtcgtcag cctgaacata acatccttgg 2941 gattacgctc cctcaaggag ataagtgatg gagatgtgat aatttcagga aacaaaaatt 3001 tgtgctatgc aaatacaata aactggaaaa aactgtttgg gacctccggt cagaaaacca 3061 aaattataag caacagaggt gaaaacagct gcaaggccac aggccaggtc tgccatgcct 3121 tgtgctcccc cgagggctgc tggggcccgg agcccaggga ctgcgtctct tgccggaatg 3181 tcagccgagg cagggaatgc gtggacaagt gcaaccttct ggagggtgag ccaagggagt 3241 ttgtggagaa ctctgagtgc atacagtgcc acccagagtg cctgcctcag gccatgaaca 3301 tcacctgcac aggacgggga ccagacaact gtatccagtg tgcccactac attgacggcc 3361 cccactgcgt caagacctgc ccggcaggag tcatgggaga aaacaacacc ctggtctgga 3421 agtacgcaga cgccggccat gtgtgccacc tgtgccatcc aaactgcacc tacggatgca 3481 ctgggccagg tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc atcgccactg 3541 ggatggtggg ggccctcctc ttgctgctgg tggtggccct ggggatcggc ctcttcatgc 3601 gaaggcgcca catcgttcgg aagcgcacgc tgcggaggct gctgcaggag agggagcttg 3661 tggagcctct tacacccagt ggagaagctc ccaaccaagc tctcttgagg atcttgaagg 3721 aaactgaatt caaaaagatc aaagtgctgg gctccggtgc gttcggcacg gtgtataagg 3781 gactctggat cccagaaggt gagaaagtta aaattcccgt cgctatcaag gaattaagag 3841 aagcaacatc tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg atggccagcg 3901 tggacaaccc ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc gtgcagctca 3961 tcacgcagct catgcccttc ggctgcctcc tggactatgt ccgggaacac aaagacaata 4021 ttggctccca gtacctgctc aactggtgtg tgcagatcgc aaagggcatg aactacttgg 4081 aggaccgtcg cttggtgcac cgcgacctgg cagccaggaa cgtactggtg aaaacaccgc 4141 agcatgtcaa gatcacagat tttgggctgg ccaaactgct gggtgcggaa gagaaagaat 4201 accatgcaga aggaggcaaa gtgcctatca agtggatggc attggaatca attttacaca 4261 gaatctatac ccaccagagt gatgtctgga gctacggggt gaccgtttgg gagttgatga 4321 cctttggatc caagccatat gacggaatcc ctgccagcga gatctcctcc atcctggaga 4381 aaggagaacg cctccctcag ccacccatat gtaccatcga tgtctacatg atcatggtca 4441 agtgctggat gatagacgca gatagtcgcc caaagttccg tgagttgatc atcgaattct 4501 ccaaaatggc ccgagacccc cagcgctacc ttgtcattca gagggatgaa agaatgcatt 4561 tgccaagtcc tacagactcc aacttctacc gtgccctgat ggatgaagaa gacatggacg 4621 acgtggtgga tgccgacgag tacctcatcc cacagcaggg cttcttcagc agcccctcca 4681 cgtcacggac tcccctcctg agctctctga gtgcaaccag caacaattcc accgtggctt 4741 gcattgatag aaatgggctg caaagctgtc ccatcaagga agacagcttc ttgcagcgat 4801 acagctcaga ccccacaggc gccttgactg aggacagcat agacgacacc ttcctcccag 4861 tgcctgaata cataaaccag tccgttccca aaaggcccgc tggctctgtg cagaatcctg 4921 tctatcacaa tcagcctctg aaccccgcgc ccagcagaga cccacactac caggaccccc 4981 acagcactgc agtgggcaac cccgagtatc tcaacactgt ccagcccacc tgtgtcaaca 5041 gcacattcga cagccctgcc cactgggccc agaaaggcag ccaccaaatt agcctggaca 5101 accctgacta ccagcaggac ttctttccca aggaagccaa gccaaatggc atctttaagg 5161 gctccacagc tgaaaatgca gaatacctaa gggtcgcgcc acaaagcagt gaatttattg 5221 gagcatagaa cccagctttc ttgtacaaag tggtgatatc caattaaccc tcactaaagg 5281 tgtaagttta aagtaagaaa tgtgccctat agtgagtcgt attaccaccc aacttttcta 5341 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc 5401 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa 5461 tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt 5521 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg 5581 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga 5641 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa 5701 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc 5761 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac 5821 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag 5881 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc 5941 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg 6001 gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatga ccgagtacaa 6061 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 6121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 6181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 6241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 6301 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 6361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 6421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 6481 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 6541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 6601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaatcg atagatccta 6661 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 6721 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 6781 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 6841 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 6901 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 6961 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 7021 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 7081 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 7141 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 7201 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt 7261 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 7321 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 7381 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7441 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7501 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7561 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7621 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg 7681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 7741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 7801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 7861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 7921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 7981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 8041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 8101 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 8161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 8221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 8281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 8341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 8401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 8461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 8521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 8581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 8641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 8701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 8761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 8821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 8881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 8941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 9001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 9061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 9121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 9181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 9241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 9301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 9361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 9421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 9481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 9541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 9601 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 9661 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 9721 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 9781 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 9841 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 9901 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 9961 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga 10021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 10081 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac 10141 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct 10201 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa 10261 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg 10321 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 10381 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 10441 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 10501 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 10561 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 10621 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 10681 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 10741 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 10801 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 10861 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg 10921 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa 10981 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc 11041 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga 11101 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat 11161 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca 11221 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa 11281 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag 11341 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc 11401 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca 11461 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 11521 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 11581 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 11641 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 11701 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 11761 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 11821 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 11881 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 11941 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 12001 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 12061 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 12121 tt //