LOCUS Exported 10711 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3R1 D560G. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3R1-D560G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10711) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10711) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10711 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3451..3477 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(5648..5672) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5725..5766 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5787..6354 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5804..6356 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5948..5965) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6175..6194 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6368..7087 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6368..7084 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6413..6434) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6674..6693) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7021..7042 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7101..7689 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7154..7174) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7572..7583) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7764..7997 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8116..8134) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8202..8306 /gene="bla" /label=AmpR promoter CDS 8307..9167 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8525..8544) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9338..9926 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9827..9846 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10080..10097 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10189..10208 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10337..10358 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10373..10403 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10411..10427 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10416..10438 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10435..10451 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10435..10451 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10501..10520 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatggtt tacccatacg atgttccaga ttacgctagt 3481 gctgaggggt accagtacag agcgctgtat gattataaaa aggaaagaga agaagatatt 3541 gacttgcact tgggtgacat attgactgtg aataaagggt ccttagtagc tcttggattc 3601 agtgatggac aggaagccag gcctgaagaa attggctggt taaatggcta taatgaaacc 3661 acaggggaaa ggggggactt tccgggaact tatgtagaat atattggaag gaaaaaaatc 3721 tcgcctccca caccaaagcc ccggccacct cggcctcttc ctgttgcacc aggttcttcg 3781 aaaactgaag cagatgttga acaacaagct ttgactctcc cggatcttgc agagcagttt 3841 gcccctcctg acattgcccc gcctcttctt atcaagctcg tggaagccat tgaaaagaaa 3901 ggtctggaat gttcaactct atacagaaca cagagctcca gcaacctggc agaattacga 3961 cagcttcttg attgtgatac accctccgtg gacttggaaa tgatcgatgt gcacgttttg 4021 gctgacgctt tcaaacgcta tctcctggac ttaccaaatc ctgtcattcc agcagccgtt 4081 tacagtgaaa tgatttcttt agctccagaa gtacaaagct ccgaagaata tattcagcta 4141 ttgaagaagc ttattaggtc gcctagcata cctcatcagt attggcttac gcttcagtat 4201 ttgttaaaac atttcttcaa gctctctcaa acctccagca aaaatctgtt gaatgcaaga 4261 gtactctctg aaattttcag ccctatgctt ttcagattct cagcagccag ctctgataat 4321 actgaaaacc tcataaaagt tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag 4381 cctgcaccag cactgcctcc taaaccacca aaacctacta ctgtagccaa caacggtatg 4441 aataacaata tgtccttaca agatgctgaa tggtactggg gagatatctc gagggaagaa 4501 gtgaatgaaa aacttcgaga tacagcagac gggacctttt tggtacgaga tgcgtctact 4561 aaaatgcatg gtgattatac tcttacacta aggaaagggg gaaataacaa attaatcaaa 4621 atatttcatc gagatgggaa atatggcttc tctgacccat taaccttcag ttctgtggtt 4681 gaattaataa accactaccg gaatgaatct ctagctcagt ataatcccaa attggatgtg 4741 aaattacttt atccagtatc caaataccaa caggatcaag ttgtcaaaga agataatatt 4801 gaagctgtag ggaaaaaatt acatgaatat aacactcagt ttcaagaaaa aagtcgagaa 4861 tatgatagat tatatgaaga atatacccgc acatcccagg aaatccaaat gaaaaggaca 4921 gctattgaag catttaatga aaccataaaa atatttgaag aacagtgcca gacccaagag 4981 cggtacagca aagaatacat agaaaagttt aaacgtgaag gcaatgagaa agaaatacaa 5041 aggattatgc ataattatga taagttgaag tctcgaatca gtgaaattat tgacagtaga 5101 agaagattgg aagaagactt gaagaagcag gcagctgagt atcgagaaat tggcaaacgt 5161 atgaacagca ttaaaccaga ccttatccag ctgagaaaga cgagagacca atacttgatg 5221 tggttgactc aaaaaggtgt tcggcaaaag aagttgaacg agtggttggg caatgaaaac 5281 actgaagacc aatattcact ggtggaagat gatgaagatt tgccccatca tgatgagaag 5341 acatggaatg ttggaagcag caaccgaaac aaagctgaaa acctgttgcg agggaagcga 5401 gatggcactt ttcttgtccg ggagagcagt aaacagggct gctatgcctg ctctgtagtg 5461 gtggacggcg aagtaaagca ttgtgtcata aacaaaacag caactggcta tggctttgcc 5521 gagccctata acttgtacag ctctctgaaa gaactggtgc tacattacca acacacctcc 5581 cttgtgcagc acaacgactc cctcaatgtc acactagcct acccagtata tgcacagcag 5641 aggcgatgcc caactttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg 5701 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat 5761 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc 5821 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct 5881 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt 5941 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct 6001 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc 6061 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag 6121 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc 6181 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga 6241 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt 6301 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc 6361 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 6421 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 6481 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 6541 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 6601 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 6661 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 6721 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 6781 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 6841 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 6901 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 6961 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 7021 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 7081 caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg 7141 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 7201 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 7261 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 7321 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 7381 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 7441 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 7501 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 7561 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 7621 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 7681 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca 7741 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 7801 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 7861 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 7921 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 7981 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 8041 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt 8101 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 8161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 8221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 8281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 8341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 8401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 8461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 8521 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc 8581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 8641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 8701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 8761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 8821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 8881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 8941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 9001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 9061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 9121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 9181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 9241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 9301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 9361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 9421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 9481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 9541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 9601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 9661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 9721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 9781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 9841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 9901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 9961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 10021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 10081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 10141 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga 10201 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 10261 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca 10321 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 10381 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 10441 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt 10501 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag 10561 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt 10621 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta 10681 tttgttaaat atgtactaca aacttagtag t //