LOCUS Exported 10790 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of BRAF G466E. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-G466E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 20..44 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 45..54 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(2349..2373) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 2381..2399 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2424..2443) /label=T7 /note="T7 promoter, forward primer" promoter complement(2425..2443) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(2449..2469) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 2523..2564 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2585..3152 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 2602..3154 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2746..2763) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2973..2992 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3166..3765 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3166..3185) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3662..3682 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 3779..4367 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3832..3852) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4250..4261) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4442..4675 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(4794..4812) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4880..4984 /gene="bla" /label=AmpR promoter CDS 4985..5845 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5203..5222) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6016..6604 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6505..6524 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6758..6775 /label=L4440 /note="L4440 vector, forward primer" primer_bind 6867..6886 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 7015..7036 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7051..7081 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7089..7105 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7094..7116 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7113..7129 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7113..7129 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7179..7198 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 7390..8023 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 8070..8195 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8692..8925 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9110..9154 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9447..9564 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 9612..10790 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 9843..10781 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 10738..10758 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cggcgctgag 61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc 121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga 181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt 241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata 301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa 361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc 421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc 481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca 541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact 601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa 661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga 721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt 781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg 841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca 901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc 961 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc 1021 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc 1081 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc 1141 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga 1201 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc 1261 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga 1321 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga 1381 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattggatc 1441 tgaatcattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt 1501 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact 1561 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact 1621 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga 1681 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga 1741 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca 1801 tgaagacctc acagtaaaaa taggtgattt tggtctagct acagtgaaat ctcgatggag 1861 tgggtcccat cagtttgaac agttgtctgg atccattttg tggatggcac cagaagtcat 1921 cagaatgcaa gataaaaatc catacagctt tcagtcagat gtatatgcat ttggaattgt 1981 tctgtatgaa ttgatgactg gacagttacc ttattcaaac atcaacaaca gggaccagat 2041 aatttttatg gtgggacgag gatacctgtc tccagatctc agtaaggtac ggagtaactg 2101 tccaaaagcc atgaagagat taatggcaga gtgcctcaaa aagaaaagag atgagagacc 2161 actctttccc caaattctcg cctctattga gctgctggcc cgctcattgc caaaaattca 2221 ccgcagtgca tcagaaccct ccttgaatcg ggctggtttc caaacagagg attttagtct 2281 atatgcttgt gcttctccaa aaacacccat ccaggcaggg ggatatggtg cgtttcctgt 2341 ccactagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt 2401 gaattgaata agaatttgtt gtgccctata gtgagtcgta ttaccaccca acttttctat 2461 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg 2521 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat 2581 gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 2641 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 2701 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 2761 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 2821 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 2881 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 2941 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 3001 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 3061 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 3121 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag 3181 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 3241 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 3301 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 3361 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 3421 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 3481 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 3541 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 3601 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 3661 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 3721 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa 3781 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 3841 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 3901 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 3961 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 4021 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 4081 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 4141 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 4201 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 4261 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 4321 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta 4381 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 4441 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 4501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 4561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 4621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 4681 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 4741 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt 4801 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 4861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 4921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 4981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 5041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 5101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 5161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 5221 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 5281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 5341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 5401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 5461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 5521 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 5581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 5641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 5701 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 5761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 5821 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 5881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 5941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 6001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 6061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 6121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 6181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 6241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 6301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 6361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 6421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 6481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 6541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 6601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 6661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 6721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 6781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6841 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 6901 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc 6961 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 7021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 7081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa 7141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 7201 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 7261 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg 7321 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 7381 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga 7441 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca 7501 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag 7561 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg 7621 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg 7681 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg 7741 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag 7801 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac 7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg 8041 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag 8101 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 8161 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 8221 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata 8281 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat 8341 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag 8401 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga 8461 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca 8521 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa 8581 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 8641 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 8701 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 8761 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 8821 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 8881 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 8941 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 9001 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta 9061 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca 9121 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg 9181 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt 9241 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca 9301 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg 9361 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat 9421 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt 9481 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa 9541 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg 9601 actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 9661 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa 9721 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 9781 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 9841 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg 9901 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg 9961 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag 10021 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct 10081 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc 10141 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg 10201 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc 10261 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg 10321 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc 10381 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct 10441 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa 10501 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca 10561 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt 10621 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc 10681 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca 10741 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga //