LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR L747S. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-L747S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 247..364 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 412..1590 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 643..1581 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1538..1558 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1610..1634 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1637..5269 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKESREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5271..5295) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5303..5321 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5346..5365) /label=T7 /note="T7 promoter, forward primer" promoter complement(5347..5365) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5371..5391) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5445..5486 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5507..6074 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5524..6076 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5668..5685) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5895..5914 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6088..6687 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6088..6107) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6584..6604 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6701..7289 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6754..6774) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7172..7183) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7364..7597 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7716..7734) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7802..7906 /gene="bla" /label=AmpR promoter CDS 7907..8767 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8125..8144) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8938..9526 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9427..9446 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9680..9697 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9789..9808 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9937..9958 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9973..10003 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10011..10027 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10016..10038 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10035..10051 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10035..10051 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10101..10120 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10312..10945 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10992..11117 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11614..11847 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12032..12076 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 61 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 121 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat 181 ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg cagtattcat 241 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa 361 ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtcgtg aggctccggt 421 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 481 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 541 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 601 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt 661 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc 721 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg 781 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc 841 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag 901 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt 961 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc 1021 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca 1081 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg 1141 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga 1201 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc 1261 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc 1321 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg 1381 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc 1441 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg 1501 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa 1561 agtttttttc ttccatttca ggtgtcgtga agcggccctg cagatatcaa caagtttgta 1621 caaaaaagca ggcaccatgc gaccctccgg gacggccggg gcagcgctcc tggcgctgct 1681 ggctgcgctc tgcccggcga gtcgggctct ggaggaaaag aaagtttgcc aaggcacgag 1741 taacaagctc acgcagttgg gcacttttga agatcatttt ctcagcctcc agaggatgtt 1801 caataactgt gaggtggtcc ttgggaattt ggaaattacc tatgtgcaga ggaattatga 1861 tctttccttc ttaaagacca tccaggaggt ggctggttat gtcctcattg ccctcaacac 1921 agtggagcga attcctttgg aaaacctgca gatcatcaga ggaaatatgt actacgaaaa 1981 ttcctatgcc ttagcagtct tatctaacta tgatgcaaat aaaaccggac tgaaggagct 2041 gcccatgaga aatttacagg aaatcctgca tggcgccgtg cggttcagca acaaccctgc 2101 cctgtgcaac gtggagagca tccagtggcg ggacatagtc agcagtgact ttctcagcaa 2161 catgtcgatg gacttccaga accacctggg cagctgccaa aagtgtgatc caagctgtcc 2221 caatgggagc tgctggggtg caggagagga gaactgccag aaactgacca aaatcatctg 2281 tgcccagcag tgctccgggc gctgccgtgg caagtccccc agtgactgct gccacaacca 2341 gtgtgctgca ggctgcacag gcccccggga gagcgactgc ctggtctgcc gcaaattccg 2401 agacgaagcc acgtgcaagg acacctgccc cccactcatg ctctacaacc ccaccacgta 2461 ccagatggat gtgaaccccg agggcaaata cagctttggt gccacctgcg tgaagaagtg 2521 tccccgtaat tatgtggtga cagatcacgg ctcgtgcgtc cgagcctgtg gggccgacag 2581 ctatgagatg gaggaagacg gcgtccgcaa gtgtaagaag tgcgaagggc cttgccgcaa 2641 agtgtgtaac ggaataggta ttggtgaatt taaagactca ctctccataa atgctacgaa 2701 tattaaacac ttcaaaaact gcacctccat cagtggcgat ctccacatcc tgccggtggc 2761 atttaggggt gactccttca cacatactcc tcctctggat ccacaggaac tggatattct 2821 gaaaaccgta aaggaaatca cagggttttt gctgattcag gcttggcctg aaaacaggac 2881 ggacctccat gcctttgaga acctagaaat catacgcggc aggaccaagc aacatggtca 2941 gttttctctt gcagtcgtca gcctgaacat aacatccttg ggattacgct ccctcaagga 3001 gataagtgat ggagatgtga taatttcagg aaacaaaaat ttgtgctatg caaatacaat 3061 aaactggaaa aaactgtttg ggacctccgg tcagaaaacc aaaattataa gcaacagagg 3121 tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc ccgagggctg 3181 ctggggcccg gagcccaggg actgcgtctc ttgccggaat gtcagccgag gcagggaatg 3241 cgtggacaag tgcaaccttc tggagggtga gccaagggag tttgtggaga actctgagtg 3301 catacagtgc cacccagagt gcctgcctca ggccatgaac atcacctgca caggacgggg 3361 accagacaac tgtatccagt gtgcccacta cattgacggc ccccactgcg tcaagacctg 3421 cccggcagga gtcatgggag aaaacaacac cctggtctgg aagtacgcag acgccggcca 3481 tgtgtgccac ctgtgccatc caaactgcac ctacggatgc actgggccag gtcttgaagg 3541 ctgtccaacg aatgggccta agatcccgtc catcgccact gggatggtgg gggccctcct 3601 cttgctgctg gtggtggccc tggggatcgg cctcttcatg cgaaggcgcc acatcgttcg 3661 gaagcgcacg ctgcggaggc tgctgcagga gagggagctt gtggagcctc ttacacccag 3721 tggagaagct cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat 3781 caaagtgctg ggctccggtg cgttcggcac ggtgtataag ggactctgga tcccagaagg 3841 tgagaaagtt aaaattcccg tcgctatcaa ggaatcaaga gaagcaacat ctccgaaagc 3901 caacaaggaa atcctcgatg aagcctacgt gatggccagc gtggacaacc cccacgtgtg 3961 ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc atcacgcagc tcatgccctt 4021 cggctgcctc ctggactatg tccgggaaca caaagacaat attggctccc agtacctgct 4081 caactggtgt gtgcagatcg caaagggcat gaactacttg gaggaccgtc gcttggtgca 4141 ccgcgacctg gcagccagga acgtactggt gaaaacaccg cagcatgtca agatcacaga 4201 ttttgggctg gccaaactgc tgggtgcgga agagaaagaa taccatgcag aaggaggcaa 4261 agtgcctatc aagtggatgg cattggaatc aattttacac agaatctata cccaccagag 4321 tgatgtctgg agctacgggg tgaccgtttg ggagttgatg acctttggat ccaagccata 4381 tgacggaatc cctgccagcg agatctcctc catcctggag aaaggagaac gcctccctca 4441 gccacccata tgtaccatcg atgtctacat gatcatggtc aagtgctgga tgatagacgc 4501 agatagtcgc ccaaagttcc gtgagttgat catcgaattc tccaaaatgg cccgagaccc 4561 ccagcgctac cttgtcattc agggggatga aagaatgcat ttgccaagtc ctacagactc 4621 caacttctac cgtgccctga tggatgaaga agacatggac gacgtggtgg atgccgacga 4681 gtacctcatc ccacagcagg gcttcttcag cagcccctcc acgtcacgga ctcccctcct 4741 gagctctctg agtgcaacca gcaacaattc caccgtggct tgcattgata gaaatgggct 4801 gcaaagctgt cccatcaagg aagacagctt cttgcagcga tacagctcag accccacagg 4861 cgccttgact gaggacagca tagacgacac cttcctccca gtgcctgaat acataaacca 4921 gtccgttccc aaaaggcccg ctggctctgt gcagaatcct gtctatcaca atcagcctct 4981 gaaccccgcg cccagcagag acccacacta ccaggacccc cacagcactg cagtgggcaa 5041 ccccgagtat ctcaacactg tccagcccac ctgtgtcaac agcacattcg acagccctgc 5101 ccactgggcc cagaaaggca gccaccaaat tagcctggac aaccctgact accagcagga 5161 cttctttccc aaggaagcca agccaaatgg catctttaag ggctccacag ctgaaaatgc 5221 agaataccta agggtcgcgc cacaaagcag tgaatttatt ggagcataga acccagcttt 5281 cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gtatttgata agagaatgaa 5341 gaagtcccta tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc 5401 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta 5461 accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc 5521 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 5581 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 5641 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 5701 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 5761 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 5821 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 5881 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 5941 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt 6001 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 6061 aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc 6121 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 6181 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 6241 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 6301 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 6361 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 6421 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 6481 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 6541 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6601 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6661 tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa 6721 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6781 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6841 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6901 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6961 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 7021 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 7081 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 7141 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 7201 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 7261 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag 7321 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 7381 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 7441 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7501 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7561 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 7621 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt 7681 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata 7741 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 7801 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 7861 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 7921 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 7981 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 8041 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 8101 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 8161 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 8221 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 8281 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 8341 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 8401 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 8461 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 8521 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 8581 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 8641 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 8701 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 8761 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 8821 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 8881 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 8941 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 9001 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 9061 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 9121 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 9181 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 9241 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 9301 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 9361 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 9421 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 9481 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 9541 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 9601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 9661 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 9721 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta 9781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 9841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt 9901 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 9961 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 10021 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt 10081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 10141 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat 10201 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt 10261 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc 10321 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 10381 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg 10441 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag 10501 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 10561 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc 10621 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact 10681 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat 10741 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 10801 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 10861 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 10921 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga 10981 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg 11041 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag 11101 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc 11161 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg 11221 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa 11281 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata 11341 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag 11401 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg 11461 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata 11521 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag 11581 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg 11641 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt 11701 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca 11761 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga 11821 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg 11881 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa 11941 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc 12001 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga 12061 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt 12121 at //