LOCUS       Exported               12122 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of EGFR L833V.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-EGFR-L833V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    258..375
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        423..1601
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          654..1592
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1549..1569
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1621..1645
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1648..5280
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRVVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5282..5306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5314..5332
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5357..5376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5358..5376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5382..5402)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5456..5497
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5518..6085
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5535..6087
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5679..5696)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5906..5925
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6099..6698
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6099..6118)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6595..6615
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6712..7300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6765..6785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7183..7194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7375..7608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7727..7745)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7813..7917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7918..8778
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8136..8155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8949..9537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9438..9457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9691..9708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9800..9819
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    9948..9969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9984..10014
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10022..10038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10027..10049
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10046..10062
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10046..10062
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10112..10131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10323..10956
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11003..11128
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11625..11858
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12043..12087
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
       61 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
      121 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
      181 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
      241 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      301 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      361 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
      421 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      481 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      541 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      601 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      661 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      721 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      781 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      841 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      901 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      961 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     1021 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     1081 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     1141 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     1201 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     1261 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1321 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1381 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1441 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1501 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1561 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     1621 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc
     1681 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc
     1741 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc
     1801 cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag
     1861 aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt
     1921 gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg
     1981 tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga
     2041 ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt gcggttcagc
     2101 aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt cagcagtgac
     2161 tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca aaagtgtgat
     2221 ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca gaaactgacc
     2281 aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc
     2341 tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg cctggtctgc
     2401 cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat gctctacaac
     2461 cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg tgccacctgc
     2521 gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt
     2581 ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg
     2641 ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata
     2701 aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc
     2761 ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa
     2821 ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct
     2881 gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag
     2941 caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc
     3001 tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat
     3061 gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata
     3121 agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc
     3181 cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga
     3241 ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag
     3301 aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc
     3361 acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc
     3421 gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca
     3481 gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca
     3541 ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg
     3601 ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc
     3661 cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg agagggagct tgtggagcct
     3721 cttacaccca gtggagaagc tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa
     3781 ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca cggtgtataa gggactctgg
     3841 atcccagaag gtgagaaagt taaaattccc gtcgctatca aggaattaag agaagcaaca
     3901 tctccgaaag ccaacaagga aatcctcgat gaagcctacg tgatggccag cgtggacaac
     3961 ccccacgtgt gccgcctgct gggcatctgc ctcacctcca ccgtgcagct catcacgcag
     4021 ctcatgccct tcggctgcct cctggactat gtccgggaac acaaagacaa tattggctcc
     4081 cagtacctgc tcaactggtg tgtgcagatc gcaaagggca tgaactactt ggaggaccgt
     4141 cgcgtggtgc accgcgacct ggcagccagg aacgtactgg tgaaaacacc gcagcatgtc
     4201 aagatcacag attttgggct ggccaaactg ctgggtgcgg aagagaaaga ataccatgca
     4261 gaaggaggca aagtgcctat caagtggatg gcattggaat caattttaca cagaatctat
     4321 acccaccaga gtgatgtctg gagctacggg gtgaccgttt gggagttgat gacctttgga
     4381 tccaagccat atgacggaat ccctgccagc gagatctcct ccatcctgga gaaaggagaa
     4441 cgcctccctc agccacccat atgtaccatc gatgtctaca tgatcatggt caagtgctgg
     4501 atgatagacg cagatagtcg cccaaagttc cgtgagttga tcatcgaatt ctccaaaatg
     4561 gcccgagacc cccagcgcta ccttgtcatt cagggggatg aaagaatgca tttgccaagt
     4621 cctacagact ccaacttcta ccgtgccctg atggatgaag aagacatgga cgacgtggtg
     4681 gatgccgacg agtacctcat cccacagcag ggcttcttca gcagcccctc cacgtcacgg
     4741 actcccctcc tgagctctct gagtgcaacc agcaacaatt ccaccgtggc ttgcattgat
     4801 agaaatgggc tgcaaagctg tcccatcaag gaagacagct tcttgcagcg atacagctca
     4861 gaccccacag gcgccttgac tgaggacagc atagacgaca ccttcctccc agtgcctgaa
     4921 tacataaacc agtccgttcc caaaaggccc gctggctctg tgcagaatcc tgtctatcac
     4981 aatcagcctc tgaaccccgc gcccagcaga gacccacact accaggaccc ccacagcact
     5041 gcagtgggca accccgagta tctcaacact gtccagccca cctgtgtcaa cagcacattc
     5101 gacagccctg cccactgggc ccagaaaggc agccaccaaa ttagcctgga caaccctgac
     5161 taccagcagg acttctttcc caaggaagcc aagccaaatg gcatctttaa gggctccaca
     5221 gctgaaaatg cagaatacct aagggtcgcg ccacaaagca gtgaatttat tggagcatag
     5281 aacccagctt tcttgtacaa agtggtgata tccaattaac cctcactaaa gggttagaat
     5341 gtatatagag ttagatccct atagtgagtc gtattaccac ccaacttttc tatacaaagt
     5401 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     5461 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagatcc
     5521 ctcccccccc cntaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     5581 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     5641 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     5701 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     5761 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     5821 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     5881 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     5941 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     6001 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     6061 tttcctttga aaaacacgat gataatatgg ccacacatat gaccgagtac aagcccacgg
     6121 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6181 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6241 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6301 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6361 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6421 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6481 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6541 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6601 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6661 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat cgatagatcc taatcaacct
     6721 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6781 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6841 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6901 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6961 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7021 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7081 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7141 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7201 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7261 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     7321 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7381 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7441 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7501 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7561 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7621 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7681 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     7741 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7801 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7861 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7921 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7981 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8041 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8101 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8161 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8221 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8281 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8341 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8401 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8461 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8521 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8581 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8641 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8701 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8761 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8821 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8881 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8941 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9001 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9061 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9601 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9661 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9721 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     9781 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9841 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     9901 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9961 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    10021 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
    10081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10141 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10201 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10261 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10321 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
    10381 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
    10441 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
    10501 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
    10561 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
    10621 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
    10681 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
    10741 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
    10801 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    10861 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    10921 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
    10981 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    11041 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    11101 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    11161 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    11221 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    11281 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    11341 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    11401 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    11461 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    11521 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    11581 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    11641 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    11701 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    11761 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    11821 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    11881 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    11941 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    12001 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    12061 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    12121 ta
//
