LOCUS Exported 12122 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR L838M. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-L838M SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12122) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 178..295 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 343..1521 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 574..1512 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1469..1489 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1541..1565 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1568..5200 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDMAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" protein_bind complement(5202..5226) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5234..5252 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5277..5296) /label=T7 /note="T7 promoter, forward primer" promoter complement(5278..5296) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5302..5322) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5376..5417 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5438..6005 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5455..6007 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5599..5616) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5826..5845 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6019..6618 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6019..6038) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6515..6535 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6632..7220 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6685..6705) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7103..7114) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7295..7528 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7647..7665) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7733..7837 /gene="bla" /label=AmpR promoter CDS 7838..8698 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8056..8075) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8869..9457 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9358..9377 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9611..9628 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9720..9739 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9868..9889 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9904..9934 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9942..9958 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9947..9969 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9966..9982 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9966..9982 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10032..10051 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10243..10876 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10923..11048 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11545..11778 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11963..12007 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg 61 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat 121 tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt 181 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 241 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt 301 ttattacagg gacagcagag atccagtttg gactagtcgt gaggctccgg tgcccgtcag 361 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 421 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 481 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 541 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg 601 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag 661 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc 721 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc 781 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc 841 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat 901 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc 961 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc 1021 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt 1081 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga 1141 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag 1201 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca 1261 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg 1321 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag 1381 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc 1441 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt 1501 cttccatttc aggtgtcgtg aagcggccct gcagatatca acaagtttgt acaaaaaagc 1561 aggcaccatg cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct 1621 ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct 1681 cacgcagttg ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg 1741 tgaggtggtc cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt 1801 cttaaagacc atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg 1861 aattcctttg gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc 1921 cttagcagtc ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag 1981 aaatttacag gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa 2041 cgtggagagc atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat 2101 ggacttccag aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag 2161 ctgctggggt gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca 2221 gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc 2281 aggctgcaca ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc 2341 cacgtgcaag gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga 2401 tgtgaacccc gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa 2461 ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat 2521 ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa 2581 cggaataggt attggtgaat ttaaagactc actctccata aatgctacga atattaaaca 2641 cttcaaaaac tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg 2701 tgactccttc acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt 2761 aaaggaaatc acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca 2821 tgcctttgag aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct 2881 tgcagtcgtc agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga 2941 tggagatgtg ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa 3001 aaaactgttt gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag 3061 ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc 3121 ggagcccagg gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa 3181 gtgcaacctt ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg 3241 ccacccagag tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa 3301 ctgtatccag tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg 3361 agtcatggga gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca 3421 cctgtgccat ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac 3481 gaatgggcct aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct 3541 ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac 3601 gctgcggagg ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc 3661 tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct 3721 gggctccggt gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt 3781 taaaattccc gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga 3841 aatcctcgat gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct 3901 gggcatctgc ctcacctcca ccgtgcagct catcacgcag ctcatgccct tcggctgcct 3961 cctggactat gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg 4021 tgtgcagatc gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacat 4081 ggcagccagg aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct 4141 ggccaaactg ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat 4201 caagtggatg gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg 4261 gagctacggg gtgaccgttt gggagttgat gacctttgga tccaagccat atgacggaat 4321 ccctgccagc gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat 4381 atgtaccatc gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg 4441 cccaaagttc cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta 4501 ccttgtcatt cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta 4561 ccgtgccctg atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat 4621 cccacagcag ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct 4681 gagtgcaacc agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg 4741 tcccatcaag gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac 4801 tgaggacagc atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc 4861 caaaaggccc gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc 4921 gcccagcaga gacccacact accaggaccc ccacagcact gcagtgggca accccgagta 4981 tctcaacact gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc 5041 ccagaaaggc agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc 5101 caaggaagcc aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct 5161 aagggtcgcg ccacaaagca gtgaatttat tggagcatag aacccagctt tcttgtacaa 5221 agtggtgata tccaattaac cctcactaaa gggtgattga atagtagatt gtttaaccct 5281 atagtgagtc gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg 5341 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc 5401 tcggtctcga ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta 5461 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca 5521 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca 5581 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg 5641 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc 5701 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata 5761 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag 5821 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc 5881 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt 5941 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat 6001 gataatatgg ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6061 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 6121 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 6181 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 6241 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 6301 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 6361 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 6421 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 6481 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 6541 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6601 cgcaagcccg gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga 6661 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 6721 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 6781 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 6841 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 6901 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 6961 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 7021 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg 7081 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 7141 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 7201 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta 7261 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 7321 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 7381 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 7441 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 7501 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 7561 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg 7621 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 7681 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 7741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 7801 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 7861 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 7921 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 7981 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 8041 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 8101 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 8161 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 8221 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 8281 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 8341 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 8401 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 8461 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 8521 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 8581 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 8641 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 8701 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 8761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 8821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 8881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 8941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 9001 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 9061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 9121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 9181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 9241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 9301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 9361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 9421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 9481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 9541 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 9601 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 9661 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt 9721 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 9781 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg 9841 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 9901 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 9961 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact 10021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg 10081 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc 10141 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta 10201 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact 10261 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 10321 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct 10381 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca 10441 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt 10501 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact 10561 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga 10621 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg 10681 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 10741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 10801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 10861 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg 10921 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc 10981 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg 11041 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc 11101 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac 11161 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac 11221 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag 11281 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca 11341 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat 11401 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa 11461 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca 11521 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg 11581 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg 11641 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca 11701 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct 11761 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc 11821 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac 11881 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga 11941 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc 12001 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat 12061 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag 12121 ag //