LOCUS Exported 12280 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of ERBB2 M916I. ACCESSION . VERSION . KEYWORDS pHAGE-ERBB2-M916I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12280) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12280) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12280 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7217..7241) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 7294..7335 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7356..7923 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7373..7925 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7517..7534) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7744..7763 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7937..8656 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7937..8653 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7982..8003) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8243..8262) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8590..8611 /label=EGFP-C /note="EGFP, forward primer" misc_feature 8670..9258 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8723..8743) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9141..9152) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9333..9566 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9685..9703) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9771..9875 /gene="bla" /label=AmpR promoter CDS 9876..10736 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10094..10113) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10907..11495 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11396..11415 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11649..11666 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11758..11777 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11906..11927 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11942..11972 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11980..11996 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11985..12007 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12004..12020 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12004..12020 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12070..12089 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gagctggcgg ccttgtgccg ctgggggctc 3481 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg 3541 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag 3601 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg 3661 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg 3721 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac 3781 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg 3841 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga 3901 ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt gtggaaggac 3961 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc 4021 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat 4081 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg 4141 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac 4201 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg 4261 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc 4321 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc 4381 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt 4441 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga 4501 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt 4561 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg 4621 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac 4681 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc 4741 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc 4801 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac 4861 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa 4921 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc 4981 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc 5041 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc 5101 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat 5161 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag 5221 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg 5281 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc 5341 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg 5401 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt 5461 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg 5521 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag 5581 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt 5641 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc 5701 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca 5761 tacgtgatgg ctggtgtggg ctccccatat gtctcccgcc ttctgggcat ctgcctgaca 5821 tccacggtgc agctggtgac acagcttatg ccctatggct gcctcttaga ccatgtccgg 5881 gaaaaccgcg gacgcctggg ctcccaggac ctgctgaact ggtgtatgca gattgccaag 5941 gggatgagct acctggagga tgtgcggctc gtacacaggg acttggccgc tcggaacgtg 6001 ctggtcaaga gtcccaacca tgtcaaaatt acagacttcg ggctggctcg gctgctggac 6061 attgacgaga cagagtacca tgcagatggg ggcaaggtgc ccatcaagtg gatggcgctg 6121 gagtccattc tccgccggcg gttcacccac cagagtgatg tgtggagtta tggtgtgact 6181 gtgtgggagc tgataacttt tggggccaaa ccttacgatg ggatcccagc ccgggagatc 6241 cctgacctgc tggaaaaggg ggagcggctg ccccagcccc ccatctgcac cattgatgtc 6301 tacatgatca tggtcaaatg ttggatgatt gactctgaat gtcggccaag attccgggag 6361 ttggtgtctg aattctcccg catggccagg gacccccagc gctttgtggt catccagaat 6421 gaggacttgg gcccagccag tcccttggac agcaccttct accgctcact gctggaggac 6481 gatgacatgg gggacctggt ggatgctgag gagtatctgg taccccagca gggcttcttc 6541 tgtccagacc ctgccccggg cgctgggggc atggtccacc acaggcaccg cagctcatct 6601 accaggagtg gcggtgggga cctgacacta gggctggagc cctctgaaga ggaggccccc 6661 aggtctccac tggcaccctc cgaaggggct ggctccgatg tatttgatgg tgacctggga 6721 atgggggcag ccaaggggct gcaaagcctc cccacacatg accccagccc tctacagcgg 6781 tacagtgagg accccacagt acccctgccc tctgagactg atggctacgt tgcccccctg 6841 acctgcagcc cccagcctga atatgtgaac cagccagatg ttcggcccca gcccccttcg 6901 ccccgagagg gccctctgcc tgctgcccga cctgctggtg ccactctgga aaggcccaag 6961 actctctccc cagggaagaa tggggtcgtc aaagacgttt ttgcctttgg gggtgccgtg 7021 gagaaccccg agtacttgac accccaggga ggagctgccc ctcagcccca ccctcctcct 7081 gccttcagcc cagccttcga caacctctat tactgggacc aggacccacc agagcggggg 7141 gctccaccca gcaccttcaa agggacacct acggcagaga acccagagta cctgggtctg 7201 gacgtgccag tgtagaaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc 7261 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc 7321 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact 7381 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 7441 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 7501 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 7561 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 7621 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 7681 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 7741 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 7801 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 7861 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 7921 taatatggcc acacatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 7981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 8041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 8101 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 8161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 8221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 8281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 8341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 8401 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 8461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 8521 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 8581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 8641 cgagctgtac aagtaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa 8701 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 8761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 8821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 8881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 8941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 9001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 9061 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 9121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 9181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 9241 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga 9301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 9361 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 9421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 9481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 9541 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 9601 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc 9661 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 9721 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 9781 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 9841 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 9901 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 9961 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 10021 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 10081 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 10141 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 10201 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 10261 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 10321 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 10381 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 10441 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 10501 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 10561 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 10621 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 10681 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 10741 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 10801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 10861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 10921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 10981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 11041 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 11101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 11161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 11221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 11281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 11341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 11401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 11461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 11521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 11581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 11641 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 11701 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat 11761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 11821 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga 11881 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 11941 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 12001 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc 12061 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 12121 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 12181 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 12241 gaaattgtat ttgttaaata tgtactacaa acttagtagt //