LOCUS Exported 10790 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of BRAF Q609E. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-Q609E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..118 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 166..1344 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 397..1335 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1292..1312 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1364..1388 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1389..1398 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3693..3717) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3725..3743 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3768..3787) /label=T7 /note="T7 promoter, forward primer" promoter complement(3769..3787) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(3793..3813) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 3867..3908 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3929..4496 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3946..4498 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4090..4107) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4317..4336 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4510..5109 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4510..4529) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5006..5026 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5123..5711 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5176..5196) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5594..5605) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5786..6019 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6138..6156) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6224..6328 /gene="bla" /label=AmpR promoter CDS 6329..7189 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6547..6566) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7360..7948 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7849..7868 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8102..8119 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8211..8230 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8359..8380 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8395..8425 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8433..8449 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8438..8460 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8457..8473 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8457..8473 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8523..8542 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8734..9367 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9414..9539 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10036..10269 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10454..10498 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt 181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc 421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg 481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct 541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc 601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta 661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta 721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg 781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga 841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg 901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa 961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga 1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct 1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt 1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact 1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt 1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt 1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa 1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc 1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc 1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac 1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat 1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca 1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat 1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt 1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt 1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt 1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt 1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct 2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt 2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca 2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc 2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca 2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc 2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc 2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt 2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt 2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg 2581 tttgtctgct accccccctg cctcattacc tggctcacta actaacgtga aagccttaca 2641 gaaatctcca ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg 2701 aatgaaaaca cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat 2761 tacagtggga caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca 2821 tggtgatgtg gcagtgaaaa tgttgaatgt gacagcacct acacctcagc agttacaagc 2881 cttcaaaaat gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat 2941 gggctattcc acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt 3001 gtatcaccat ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc 3061 acgacagact gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct 3121 caagagtaat aatatatttc ttcatgaaga cctcacagta aaaataggtg attttggtct 3181 agctacagtg aaatctcgat ggagtgggtc ccatgagttt gaacagttgt ctggatccat 3241 tttgtggatg gcaccagaag tcatcagaat gcaagataaa aatccataca gctttcagtc 3301 agatgtatat gcatttggaa ttgttctgta tgaattgatg actggacagt taccttattc 3361 aaacatcaac aacagggacc agataatttt tatggtggga cgaggatacc tgtctccaga 3421 tctcagtaag gtacggagta actgtccaaa agccatgaag agattaatgg cagagtgcct 3481 caaaaagaaa agagatgaga gaccactctt tccccaaatt ctcgcctcta ttgagctgct 3541 ggcccgctca ttgccaaaaa ttcaccgcag tgcatcagaa ccctccttga atcgggctgg 3601 tttccaaaca gaggatttta gtctatatgc ttgtgcttct ccaaaaacac ccatccaggc 3661 agggggatat ggtgcgtttc ctgtccacta gaacccagct ttcttgtaca aagtggtgat 3721 atccaattaa ccctcactaa aggtaaagag tgatgtaaat agaagttccc tatagtgagt 3781 cgtattacca cccaactttt ctatacaaag tggttgatat ccagcacagt ggcggccgct 3841 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg 3901 attctacgcg taccggttag taatgagatc cctccccccc ccctaacgtt actggccgaa 3961 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 4021 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 4081 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 4141 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 4201 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 4261 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 4321 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 4381 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 4441 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 4501 gccacacata tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 4561 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 4621 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 4681 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 4741 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 4801 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 4861 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 4921 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4981 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 5041 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 5101 ggtgcctaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac 5161 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 5221 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 5281 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 5341 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 5401 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 5461 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 5521 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5581 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5641 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 5701 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc 5761 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat 5821 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 5881 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 5941 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6001 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6061 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca 6121 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 6181 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 6241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 6301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 6361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 6421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 6481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 6541 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 6601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 6661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 6721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 6781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 6841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 6901 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 6961 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 7021 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 7081 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 7141 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 7201 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 7261 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 7321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 7381 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 7441 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 7501 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 7561 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 7621 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 7681 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 7741 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 7801 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 7861 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 7921 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 7981 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 8041 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 8101 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 8161 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca 8221 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 8281 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg 8341 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 8401 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 8461 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag 8521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca 8581 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct 8641 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg 8701 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa 8761 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga 8821 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 8881 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 8941 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg 9001 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 9061 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 9121 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc 9181 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 9241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 9301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 9361 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc 9421 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga 9481 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca 9541 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa 9601 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag 9661 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac 9721 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct 9781 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg 9841 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc 9901 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa 9961 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa 10021 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat 10081 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca 10141 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt 10201 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca 10261 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg 10321 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga 10381 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca 10441 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg 10501 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg 10561 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca 10621 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc 10681 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa 10741 cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa //