LOCUS Exported 10708 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3R1 L380delL. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3R1-L380delL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10708) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10708) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10708 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3451..3477 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(5645..5669) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5722..5763 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5784..6351 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5801..6353 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5945..5962) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6172..6191 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6365..7084 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6365..7081 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6410..6431) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6671..6690) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7018..7039 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7098..7686 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7151..7171) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7569..7580) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7761..7994 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8113..8131) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8199..8303 /gene="bla" /label=AmpR promoter CDS 8304..9164 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8522..8541) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9335..9923 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9824..9843 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10077..10094 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10186..10205 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10334..10355 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10370..10400 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10408..10424 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10413..10435 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10432..10448 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10432..10448 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10498..10517 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatggtt tacccatacg atgttccaga ttacgctagt 3481 gctgaggggt accagtacag agcgctgtat gattataaaa aggaaagaga agaagatatt 3541 gacttgcact tgggtgacat attgactgtg aataaagggt ccttagtagc tcttggattc 3601 agtgatggac aggaagccag gcctgaagaa attggctggt taaatggcta taatgaaacc 3661 acaggggaaa ggggggactt tccgggaact tatgtagaat atattggaag gaaaaaaatc 3721 tcgcctccca caccaaagcc ccggccacct cggcctcttc ctgttgcacc aggttcttcg 3781 aaaactgaag cagatgttga acaacaagct ttgactctcc cggatcttgc agagcagttt 3841 gcccctcctg acattgcccc gcctcttctt atcaagctcg tggaagccat tgaaaagaaa 3901 ggtctggaat gttcaactct atacagaaca cagagctcca gcaacctggc agaattacga 3961 cagcttcttg attgtgatac accctccgtg gacttggaaa tgatcgatgt gcacgttttg 4021 gctgacgctt tcaaacgcta tctcctggac ttaccaaatc ctgtcattcc agcagccgtt 4081 tacagtgaaa tgatttcttt agctccagaa gtacaaagct ccgaagaata tattcagcta 4141 ttgaagaagc ttattaggtc gcctagcata cctcatcagt attggcttac gcttcagtat 4201 ttgttaaaac atttcttcaa gctctctcaa acctccagca aaaatctgtt gaatgcaaga 4261 gtactctctg aaattttcag ccctatgctt ttcagattct cagcagccag ctctgataat 4321 actgaaaacc tcataaaagt tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag 4381 cctgcaccag cactgcctcc taaaccacca aaacctacta ctgtagccaa caacggtatg 4441 aataacaata tgtccttaca agatgctgaa tggtactggg gagatatctc gagggaagaa 4501 gtgaatgaaa aacttcgaga tacagcagac gggacctttt tggtacgaga tgcgtctact 4561 aaaatgcatg gtgattatac tcttacacta aggaaagggg gaaataacaa aatcaaaata 4621 tttcatcgag atgggaaata tggcttctct gacccattaa ccttcagttc tgtggttgaa 4681 ttaataaacc actaccggaa tgaatctcta gctcagtata atcccaaatt ggatgtgaaa 4741 ttactttatc cagtatccaa ataccaacag gatcaagttg tcaaagaaga taatattgaa 4801 gctgtaggga aaaaattaca tgaatataac actcagtttc aagaaaaaag tcgagaatat 4861 gatagattat atgaagaata tacccgcaca tcccaggaaa tccaaatgaa aaggacagct 4921 attgaagcat ttaatgaaac cataaaaata tttgaagaac agtgccagac ccaagagcgg 4981 tacagcaaag aatacataga aaagtttaaa cgtgaaggca atgagaaaga aatacaaagg 5041 attatgcata attatgataa gttgaagtct cgaatcagtg aaattattga cagtagaaga 5101 agattggaag aagacttgaa gaagcaggca gctgagtatc gagaaattga caaacgtatg 5161 aacagcatta aaccagacct tatccagctg agaaagacga gagaccaata cttgatgtgg 5221 ttgactcaaa aaggtgttcg gcaaaagaag ttgaacgagt ggttgggcaa tgaaaacact 5281 gaagaccaat attcactggt ggaagatgat gaagatttgc cccatcatga tgagaagaca 5341 tggaatgttg gaagcagcaa ccgaaacaaa gctgaaaacc tgttgcgagg gaagcgagat 5401 ggcacttttc ttgtccggga gagcagtaaa cagggctgct atgcctgctc tgtagtggtg 5461 gacggcgaag taaagcattg tgtcataaac aaaacagcaa ctggctatgg ctttgccgag 5521 ccctataact tgtacagctc tctgaaagaa ctggtgctac attaccaaca cacctccctt 5581 gtgcagcaca acgactccct caatgtcaca ctagcctacc cagtatatgc acagcagagg 5641 cgatgcccaa ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt 5701 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct 5761 acgcgtaccg gttagtaatg agatccctcc ccccccccta acgttactgg ccgaagccgc 5821 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt 5881 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt 5941 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg 6001 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca 6061 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg 6121 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc 6181 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct 6241 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta 6301 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac 6361 acatatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 6421 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 6481 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 6541 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 6601 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 6661 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 6721 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 6781 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 6841 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 6901 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 6961 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 7021 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 7081 gtaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 7141 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 7201 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 7261 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 7321 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 7381 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 7441 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt 7501 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 7561 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 7621 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 7681 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 7741 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct 7801 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 7861 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 7921 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 7981 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 8041 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt 8101 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 8161 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 8221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 8281 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 8341 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 8401 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 8461 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 8521 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca 8581 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 8641 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 8701 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 8761 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 8821 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 8881 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 8941 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 9001 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 9061 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 9121 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 9181 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 9241 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 9301 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 9361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 9421 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 9481 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 9541 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 9601 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 9661 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 9721 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 9781 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 9841 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 9901 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 9961 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 10021 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 10081 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 10141 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc 10201 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 10261 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg 10321 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 10381 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 10441 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 10501 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta 10561 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt 10621 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt 10681 gttaaatatg tactacaaac ttagtagt //