LOCUS Exported 10696 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3R1 S460_D464delSREYD. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3R1-S460_D464delSREYD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10696) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10696 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3451..3477 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(5633..5657) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5710..5751 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5772..6339 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5789..6341 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5933..5950) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6160..6179 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6353..7072 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6353..7069 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6398..6419) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6659..6678) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7006..7027 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7086..7674 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7139..7159) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7557..7568) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7749..7982 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8101..8119) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8187..8291 /gene="bla" /label=AmpR promoter CDS 8292..9152 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8510..8529) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9323..9911 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9812..9831 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10065..10082 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10174..10193 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10322..10343 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10358..10388 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10396..10412 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10401..10423 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10420..10436 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10420..10436 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10486..10505 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatggtt tacccatacg atgttccaga ttacgctagt 3481 gctgaggggt accagtacag agcgctgtat gattataaaa aggaaagaga agaagatatt 3541 gacttgcact tgggtgacat attgactgtg aataaagggt ccttagtagc tcttggattc 3601 agtgatggac aggaagccag gcctgaagaa attggctggt taaatggcta taatgaaacc 3661 acaggggaaa ggggggactt tccgggaact tatgtagaat atattggaag gaaaaaaatc 3721 tcgcctccca caccaaagcc ccggccacct cggcctcttc ctgttgcacc aggttcttcg 3781 aaaactgaag cagatgttga acaacaagct ttgactctcc cggatcttgc agagcagttt 3841 gcccctcctg acattgcccc gcctcttctt atcaagctcg tggaagccat tgaaaagaaa 3901 ggtctggaat gttcaactct atacagaaca cagagctcca gcaacctggc agaattacga 3961 cagcttcttg attgtgatac accctccgtg gacttggaaa tgatcgatgt gcacgttttg 4021 gctgacgctt tcaaacgcta tctcctggac ttaccaaatc ctgtcattcc agcagccgtt 4081 tacagtgaaa tgatttcttt agctccagaa gtacaaagct ccgaagaata tattcagcta 4141 ttgaagaagc ttattaggtc gcctagcata cctcatcagt attggcttac gcttcagtat 4201 ttgttaaaac atttcttcaa gctctctcaa acctccagca aaaatctgtt gaatgcaaga 4261 gtactctctg aaattttcag ccctatgctt ttcagattct cagcagccag ctctgataat 4321 actgaaaacc tcataaaagt tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag 4381 cctgcaccag cactgcctcc taaaccacca aaacctacta ctgtagccaa caacggtatg 4441 aataacaata tgtccttaca agatgctgaa tggtactggg gagatatctc gagggaagaa 4501 gtgaatgaaa aacttcgaga tacagcagac gggacctttt tggtacgaga tgcgtctact 4561 aaaatgcatg gtgattatac tcttacacta aggaaagggg gaaataacaa attaatcaaa 4621 atatttcatc gagatgggaa atatggcttc tctgacccat taaccttcag ttctgtggtt 4681 gaattaataa accactaccg gaatgaatct ctagctcagt ataatcccaa attggatgtg 4741 aaattacttt atccagtatc caaataccaa caggatcaag ttgtcaaaga agataatatt 4801 gaagctgtag ggaaaaaatt acatgaatat aacactcagt ttcaagaaaa aagattatat 4861 gaagaatata cccgcacatc ccaggaaatc caaatgaaaa ggacagctat tgaagcattt 4921 aatgaaacca taaaaatatt tgaagaacag tgccagaccc aagagcggta cagcaaagaa 4981 tacatagaaa agtttaaacg tgaaggcaat gagaaagaaa tacaaaggat tatgcataat 5041 tatgataagt tgaagtctcg aatcagtgaa attattgaca gtagaagaag attggaagaa 5101 gacttgaaga agcaggcagc tgagtatcga gaaattgaca aacgtatgaa cagcattaaa 5161 ccagacctta tccagctgag aaagacgaga gaccaatact tgatgtggtt gactcaaaaa 5221 ggtgttcggc aaaagaagtt gaacgagtgg ttgggcaatg aaaacactga agaccaatat 5281 tcactggtgg aagatgatga agatttgccc catcatgatg agaagacatg gaatgttgga 5341 agcagcaacc gaaacaaagc tgaaaacctg ttgcgaggga agcgagatgg cacttttctt 5401 gtccgggaga gcagtaaaca gggctgctat gcctgctctg tagtggtgga cggcgaagta 5461 aagcattgtg tcataaacaa aacagcaact ggctatggct ttgccgagcc ctataacttg 5521 tacagctctc tgaaagaact ggtgctacat taccaacaca cctcccttgt gcagcacaac 5581 gactccctca atgtcacact agcctaccca gtatatgcac agcagaggcg atgcccaact 5641 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg 5701 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt 5761 tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc 5821 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg 5881 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc 5941 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga 6001 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg 6061 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag 6121 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc 6181 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct 6241 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac 6301 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac atatggtgag 6361 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 6421 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 6481 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 6541 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 6601 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 6661 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 6721 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 6781 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 6841 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 6901 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 6961 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 7021 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaatcgatag 7081 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 7141 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 7201 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 7261 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 7321 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 7381 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 7441 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 7501 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 7561 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 7621 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga 7681 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 7741 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 7801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 7861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 7921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 7981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 8041 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg 8101 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 8161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 8221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 8281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 8341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 8401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 8461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 8521 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 8581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 8641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 8701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 8761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 8821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 8881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 8941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 9001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 9061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 9121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 9181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 9241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 9301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 9361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 9421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 9481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 9541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 9601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 9661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 9721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 9781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 9841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 9901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 9961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 10021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 10081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 10141 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 10201 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 10261 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 10321 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 10381 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga 10441 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 10501 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc 10561 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct 10621 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 10681 ctacaaactt agtagt //