LOCUS Exported 10790 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of BRAF M53I. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-M53I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 48..1226 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 279..1217 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1174..1194 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1246..1270 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1271..1280 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3575..3599) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3607..3625 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3650..3669) /label=T7 /note="T7 promoter, forward primer" promoter complement(3651..3669) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(3675..3695) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 3749..3790 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3811..4378 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3828..4380 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3972..3989) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4199..4218 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4392..4991 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4392..4411) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4888..4908 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5005..5593 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5058..5078) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5476..5487) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5668..5901 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6020..6038) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6106..6210 /gene="bla" /label=AmpR promoter CDS 6211..7071 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6429..6448) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7242..7830 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7731..7750 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7984..8001 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8093..8112 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8241..8262 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8277..8307 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8315..8331 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8320..8342 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8339..8355 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8339..8355 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8405..8424 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8616..9249 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9296..9421 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9918..10151 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10336..10380 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10673..10790 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc 61 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 121 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 241 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc 301 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc 361 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 421 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg 481 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 541 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 601 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 661 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 721 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 781 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 841 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 901 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 961 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 1021 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 1081 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 1141 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 1201 tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa 1261 aaagcaggca ccatggcggc gctgagcggt ggcggtggtg gcggcgcgga gccgggccag 1321 gctctgttca acggggacat ggagcccgag gccggcgccg gcgccggcgc cgcggcctct 1381 tcggctgcgg accctgccat tccggaggag gtgtggaata tcaaacaaat tattaagttg 1441 acacaggaac atatagaggc cctattggac aaatttggtg gggagcataa tccaccatca 1501 atatatctgg aggcctatga agaatacacc agcaagctag atgcactcca acaaagagaa 1561 caacagttat tggaatctct ggggaacgga actgattttt ctgtttctag ctctgcatca 1621 atggataccg ttacatcttc ttcctcttct agcctttcag tgctaccttc atctctttca 1681 gtttttcaaa atcccacaga tgtggcacgg agcaacccca agtcaccaca aaaacctatc 1741 gttagagtct tcctgcccaa caaacagagg acagtggtac ctgcaaggtg tggagttaca 1801 gtccgagaca gtctaaagaa agcactgatg atgagaggtc taatcccaga gtgctgtgct 1861 gtttacagaa ttcaggatgg agagaagaaa ccaattggtt gggacactga tatttcctgg 1921 cttactggag aagaattgca tgtggaagtg ttggagaatg ttccacttac aacacacaac 1981 tttgtacgaa aaacgttttt caccttagca ttttgtgact tttgtcgaaa gctgcttttc 2041 cagggtttcc gctgtcaaac atgtggttat aaatttcacc agcgttgtag tacagaagtt 2101 ccactgatgt gtgttaatta tgaccaactt gatttgctgt ttgtctccaa gttctttgaa 2161 caccacccaa taccacagga agaggcgtcc ttagcagaga ctgccctaac atctggatca 2221 tccccttccg cacccgcctc ggactctatt gggccccaaa ttctcaccag tccgtctcct 2281 tcaaaatcca ttccaattcc acagcccttc cgaccagcag atgaagatca tcgaaatcaa 2341 tttgggcaac gagaccgatc ctcatcagct cccaatgtgc atataaacac aatagaacct 2401 gtcaatattg atgacttgat tagagaccaa ggatttcgtg gtgatggagg atcaaccaca 2461 ggtttgtctg ctaccccccc tgcctcatta cctggctcac taactaacgt gaaagcctta 2521 cagaaatctc caggacctca gcgagaaagg aagtcatctt catcctcaga agacaggaat 2581 cgaatgaaaa cacttggtag acgggactcg agtgatgatt gggagattcc tgatgggcag 2641 attacagtgg gacaaagaat tggatctgga tcatttggaa cagtctacaa gggaaagtgg 2701 catggtgatg tggcagtgaa aatgttgaat gtgacagcac ctacacctca gcagttacaa 2761 gccttcaaaa atgaagtagg agtactcagg aaaacacgac atgtgaatat cctactcttc 2821 atgggctatt ccacaaagcc acaactggct attgttaccc agtggtgtga gggctccagc 2881 ttgtatcacc atctccatat cattgagacc aaatttgaga tgatcaaact tatagatatt 2941 gcacgacaga ctgcacaggg catggattac ttacacgcca agtcaatcat ccacagagac 3001 ctcaagagta ataatatatt tcttcatgaa gacctcacag taaaaatagg tgattttggt 3061 ctagctacag tgaaatctcg atggagtggg tcccatcagt ttgaacagtt gtctggatcc 3121 attttgtgga tggcaccaga agtcatcaga atgcaagata aaaatccata cagctttcag 3181 tcagatgtat atgcatttgg aattgttctg tatgaattga tgactggaca gttaccttat 3241 tcaaacatca acaacaggga ccagataatt tttatggtgg gacgaggata cctgtctcca 3301 gatctcagta aggtacggag taactgtcca aaagccatga agagattaat ggcagagtgc 3361 ctcaaaaaga aaagagatga gagaccactc tttccccaaa ttctcgcctc tattgagctg 3421 ctggcccgct cattgccaaa aattcaccgc agtgcatcag aaccctcctt gaatcgggct 3481 ggtttccaaa cagaggattt tagtctatat gcttgtgctt ctccaaaaac acccatccag 3541 gcagggggat atggtgcgtt tcctgtccac tagaacccag ctttcttgta caaagtggtg 3601 atatccaatt aaccctcact aaaggtatta tgagaaagtt gaatagtagc cctatagtga 3661 gtcgtattac cacccaactt ttctatacaa agtggttgat atccagcaca gtggcggccg 3721 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct 3781 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg 3841 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc 3901 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag 3961 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt 4021 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa 4081 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc 4141 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg 4201 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat 4261 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa 4321 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata 4381 tggccacaca tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 4441 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 4501 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 4561 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 4621 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 4681 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 4741 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 4801 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 4861 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 4921 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 4981 ccggtgccta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg 5041 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5101 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5161 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5221 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5281 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 5341 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 5401 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5461 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5521 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 5581 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta 5641 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 5701 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 5761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 5821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 5881 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 5941 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat 6001 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 6061 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 6121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 6181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 6241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 6301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 6361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 6421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt 6481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 6541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 6601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 6661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 6721 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 6781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 6841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 6901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 6961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 7021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 7081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 7141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 7201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 7261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 7321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 7381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 7441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 7501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 7561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 7621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 7681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 7741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 7801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 7861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 7921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 7981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 8041 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg 8101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 8161 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg 8221 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 8281 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 8341 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct 8401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact 8461 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac 8521 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat 8581 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag 8641 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca 8701 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct 8761 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt 8821 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt 8881 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa 8941 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg 9001 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg 9061 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 9121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 9181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 9241 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc 9301 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt 9361 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt 9421 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg 9481 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc 9541 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca 9601 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct 9661 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga 9721 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga 9781 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta 9841 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa 9901 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact 9961 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg 10021 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca 10081 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat 10141 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct 10201 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg 10261 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg 10321 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg 10381 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta 10441 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg 10501 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg 10561 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg 10621 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa 10681 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag 10741 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt //