LOCUS Exported 12518 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of ERBB3 G40S. ACCESSION . VERSION . KEYWORDS pHAGE-ERBB3-G40S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12518) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12518) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12518 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7478..7502) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7510..7528 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7553..7572) /label=T7 /note="T7 promoter, forward primer" promoter complement(7554..7572) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(7578..7598) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 7652..7693 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7714..8281 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7731..8283 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7875..7892) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 8102..8121 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8295..8894 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8295..8314) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8791..8811 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8908..9496 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8961..8981) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9379..9390) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9571..9804 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9923..9941) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10009..10113 /gene="bla" /label=AmpR promoter CDS 10114..10974 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10332..10351) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11145..11733 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11634..11653 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11887..11904 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11996..12015 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 12144..12165 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12180..12210 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12218..12234 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12223..12245 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12242..12258 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12242..12258 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12308..12327 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg agggcgaacg acgctctgca ggtgctgggc 3481 ttgcttttca gcctggcccg gggctccgag gtgggcaact ctcaggcagt gtgtcctggg 3541 actctgaatg gcctgagtgt gaccagcgat gctgagaacc aataccagac actgtacaag 3601 ctctacgaga ggtgtgaggt ggtgatgggg aaccttgaga ttgtgctcac gggacacaat 3661 gccgacctct ccttcctgca gtggattcga gaagtgacag gctatgtcct cgtggccatg 3721 aatgaattct ctactctacc attgcccaac ctccgcgtgg tgcgagggac ccaggtctac 3781 gatgggaagt ttgccatctt cgtcatgttg aactataaca ccaactccag ccacgctctg 3841 cgccagctcc gcttgactca gctcaccgag attctgtcag ggggtgttta tattgagaag 3901 aacgataagc tttgtcacat ggacacaatt gactggaggg acatcgtgag ggaccgagat 3961 gctgagatag tggtgaagga caatggcaga agctgtcccc cctgtcatga ggtttgcaag 4021 gggcgatgct ggggtcctgg atcagaagac tgccagacat tgaccaagac catctgtgct 4081 cctcagtgta atggtcactg ctttgggccc aaccccaacc agtgctgcca tgatgagtgt 4141 gccgggggct gctcaggccc tcaggacaca gactgctttg cctgccggca cttcaatgac 4201 agtggagcct gtgtacctcg ctgtccacag cctcttgtct acaacaagct aactttccag 4261 ctggaaccca atccccacac caagtatcag tatggaggag tttgtgtagc cagctgtccc 4321 cataactttg tggtggatca aacatcctgt gtcagggcct gtcctcctga caagatggaa 4381 gtagataaaa atgggctcaa gatgtgtgag ccttgtgggg gactatgtcc caaagcctgt 4441 gagggaacag gctctgggag ccgcttccag actgtggact cgagcaacat tgatggattt 4501 gtgaactgca ccaagatcct gggcaacctg gactttctga tcaccggcct caatggagac 4561 ccctggcaca agatccctgc cctggaccca gagaagctca atgtcttccg gacagtacgg 4621 gagatcacag gttacctgaa catccagtcc tggccgcccc acatgcacaa cttcagtgtt 4681 ttttccaatt tgacaaccat tggaggcaga agcctctaca accggggctt ctcattgttg 4741 atcatgaaga acttgaatgt cacatctctg ggcttccgat ccctgaagga aattagtgct 4801 gggcgtatct atataagtgc caataggcag ctctgctacc accactcttt gaactggacc 4861 aaggtgcttc gggggcctac ggaagagcga ctagacatca agcataatcg gccgcgcaga 4921 gactgcgtgg cagagggcaa agtgtgtgac ccactgtgct cctctggggg atgctggggc 4981 ccaggccctg gtcagtgctt gtcctgtcga aattatagcc gaggaggtgt ctgtgtgacc 5041 cactgcaact ttctgaatgg ggagcctcga gaatttgccc atgaggccga atgcttctcc 5101 tgccacccgg aatgccaacc catggagggc actgccacat gcaatggctc gggctctgat 5161 acttgtgctc aatgtgccca ttttcgagat gggccccact gtgtgagcag ctgcccccat 5221 ggagtcctag gtgccaaggg cccaatctac aagtacccag atgttcagaa tgaatgtcgg 5281 ccctgccatg agaactgcac ccaggggtgt aaaggaccag agcttcaaga ctgtttagga 5341 caaacactgg tgctgatcgg caaaacccat ctgacaatgg ctttgacagt gatagcagga 5401 ttggtagtga ttttcatgat gctgggcggc acttttctct actggcgtgg gcgccggatt 5461 cagaataaaa gggctatgag gcgatacttg gaacggggtg agagcataga gcctctggac 5521 cccagtgaga aggctaacaa agtcttggcc agaatcttca aagagacaga gctaaggaag 5581 cttaaagtgc ttggctcggg tgtctttgga actgtgcaca aaggagtgtg gatccctgag 5641 ggtgaatcaa tcaagattcc agtctgcatt aaagtcattg aggacaagag tggacggcag 5701 agttttcaag ctgtgacaga tcatatgctg gccattggca gcctggacca tgcccacatt 5761 gtaaggctgc tgggactatg cccagggtca tctctgcagc ttgtcactca atatttgcct 5821 ctgggttctc tgctggatca tgtgagacaa caccgggggg cactggggcc acagctgctg 5881 ctcaactggg gagtacaaat tgccaaggga atgtactacc ttgaggaaca tggtatggtg 5941 catagaaacc tggctgcccg aaacgtgcta ctcaagtcac ccagtcaggt tcaggtggca 6001 gattttggtg tggctgacct gctgcctcct gatgataagc agctgctata cagtgaggcc 6061 aagactccaa ttaagtggat ggcccttgag agtatccact ttgggaaata cacacaccag 6121 agtgatgtct ggagctatgg tgtgacagtt tgggagttga tgaccttcgg ggcagagccc 6181 tatgcagggc tacgattggc tgaagtacca gacctgctag agaaggggga gcggttggca 6241 cagccccaga tctgcacaat tgatgtctac atggtgatgg tcaagtgttg gatgattgat 6301 gagaacattc gcccaacctt taaagaacta gccaatgagt tcaccaggat ggcccgagac 6361 ccaccacggt atctggtcat aaagagagag agtgggcctg gaatagcccc tgggccagag 6421 ccccatggtc tgacaaacaa gaagctagag gaagtagagc tggagccaga actagaccta 6481 gacctagact tggaagcaga ggaggacaac ctggcaacca ccacactggg ctccgccctc 6541 agcctaccag ttggaacact taatcggcca cgtgggagcc agagcctttt aagtccatca 6601 tctggataca tgcccatgaa ccagggtaat cttggggagt cttgccagga gtctgcagtt 6661 tctgggagca gtgaacggtg cccccgtcca gtctctctac acccaatgcc acggggatgc 6721 ctggcatcag agtcatcaga ggggcatgta acaggctctg aggctgagct ccaggagaaa 6781 gtgtcaatgt gtaggagccg gagcaggagc cggagcccac ggccacgcgg agatagcgcc 6841 taccattccc agcgccacag tctgctgact cctgttaccc cactctcccc acccgggtta 6901 gaggaagagg atgtcaacgg ttatgtcatg ccagatacac acctcaaagg tactccctcc 6961 tcccgggaag gcaccctttc ttcagtgggt ctcagttctg tcctgggtac tgaagaagaa 7021 gatgaagatg aggagtatga atacatgaac cggaggagaa ggcacagtcc acctcatccc 7081 cctaggccaa gttcccttga ggagctgggt tatgagtaca tggatgtggg gtcagacctc 7141 agtgcctctc tgggcagcac acagagttgc ccactccacc ctgtacccat catgcccact 7201 gcaggcacaa ctccagatga agactatgaa tatatgaatc ggcaacgaga tggaggtggt 7261 cctgggggtg attatgcagc catgggggcc tgcccagcat ctgagcaagg gtatgaagag 7321 atgagagctt ttcaggggcc tggacatcag gccccccatg tccattatgc ccgcctaaaa 7381 actctacgta gcttagaggc tacagactct gcctttgata accctgatta ctggcatagc 7441 aggcttttcc ccaaggctaa tgcccagaga acgtagaacc cagctttctt gtacaaagtg 7501 gtgatatcca attaaccctc actaaaggtt gtatgtgagt ttagattaat gaccctatag 7561 tgagtcgtat taccacccaa cttttctata caaagtggtt gatatccagc acagtggcgg 7621 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg 7681 tctcgattct acgcgtaccg gttagtaatg agatccctcc cccccccnta acgttactgg 7741 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 7801 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 7861 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 7921 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 7981 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 8041 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 8101 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 8161 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 8221 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 8281 atatggccac acatatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 8341 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 8401 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 8461 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 8521 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 8581 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 8641 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 8701 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 8761 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 8821 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 8881 agcccggtgc ctaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga 8941 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 9001 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 9061 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 9121 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 9181 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 9241 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 9301 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 9361 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 9421 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 9481 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc 9541 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 9601 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 9661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 9721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 9781 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 9841 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta 9901 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 9961 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 10021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 10081 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 10141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 10201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 10261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 10321 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 10381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 10441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 10501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 10561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 10621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 10681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 10741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 10801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 10861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 10921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 10981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 11041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 11101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 11161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 11221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 11281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 11341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 11401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 11461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 11521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 11581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 11641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 11701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 11761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 11821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 11881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 11941 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt 12001 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 12061 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa 12121 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 12181 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 12241 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat 12301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata 12361 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat 12421 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 12481 aattgtattt gttaaatatg tactacaaac ttagtagt //