LOCUS Exported 10789 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of BRAF P403Lfs*8. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-P403Lfs*8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10789) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10789) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10789 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..118 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 166..1344 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 397..1335 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1292..1312 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1364..1388 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1389..1398 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3692..3716) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3724..3742 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3767..3786) /label=T7 /note="T7 promoter, forward primer" promoter complement(3768..3786) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(3792..3812) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 3866..3907 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3928..4495 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3945..4497 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4089..4106) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4316..4335 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4509..5108 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4509..4528) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5005..5025 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5122..5710 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5175..5195) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5593..5604) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5785..6018 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6137..6155) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6223..6327 /gene="bla" /label=AmpR promoter CDS 6328..7188 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6546..6565) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7359..7947 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7848..7867 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8101..8118 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8210..8229 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8358..8379 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8394..8424 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8432..8448 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8437..8459 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8456..8472 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8456..8472 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8522..8541 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8733..9366 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9413..9538 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10035..10268 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10453..10497 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt 181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc 421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg 481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct 541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc 601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta 661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta 721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg 781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga 841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg 901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa 961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga 1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct 1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt 1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact 1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt 1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt 1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa 1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc 1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc 1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac 1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat 1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca 1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat 1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt 1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt 1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt 1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt 1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct 2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt 2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca 2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc 2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca 2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc 2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc 2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt 2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt 2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg 2581 tttgtctgct acccccctgc ctcattacct ggctcactaa ctaacgtgaa agccttacag 2641 aaatctccag gacctcagcg agaaaggaag tcatcttcat cctcagaaga caggaatcga 2701 atgaaaacac ttggtagacg ggactcgagt gatgattggg agattcctga tgggcagatt 2761 acagtgggac aaagaattgg atctggatca tttggaacag tctacaaggg aaagtggcat 2821 ggtgatgtgg cagtgaaaat gttgaatgtg acagcaccta cacctcagca gttacaagcc 2881 ttcaaaaatg aagtaggagt actcaggaaa acacgacatg tgaatatcct actcttcatg 2941 ggctattcca caaagccaca actggctatt gttacccagt ggtgtgaggg ctccagcttg 3001 tatcaccatc tccatatcat tgagaccaaa tttgagatga tcaaacttat agatattgca 3061 cgacagactg cacagggcat ggattactta cacgccaagt caatcatcca cagagacctc 3121 aagagtaata atatatttct tcatgaagac ctcacagtaa aaataggtga ttttggtcta 3181 gctacagtga aatctcgatg gagtgggtcc catcagtttg aacagttgtc tggatccatt 3241 ttgtggatgg caccagaagt catcagaatg caagataaaa atccatacag ctttcagtca 3301 gatgtatatg catttggaat tgttctgtat gaattgatga ctggacagtt accttattca 3361 aacatcaaca acagggacca gataattttt atggtgggac gaggatacct gtctccagat 3421 ctcagtaagg tacggagtaa ctgtccaaaa gccatgaaga gattaatggc agagtgcctc 3481 aaaaagaaaa gagatgagag accactcttt ccccaaattc tcgcctctat tgagctgctg 3541 gcccgctcat tgccaaaaat tcaccgcagt gcatcagaac cctccttgaa tcgggctggt 3601 ttccaaacag aggattttag tctatatgct tgtgcttctc caaaaacacc catccaggca 3661 gggggatatg gtgcgtttcc tgtccactag aacccagctt tcttgtacaa agtggtgata 3721 tccaattaac cctcactaaa gggataagaa agtgaaatgt aaattgccct atagtgagtc 3781 gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc 3841 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga 3901 ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag 3961 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc 4021 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg 4081 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc 4141 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc 4201 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa 4261 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct 4321 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg 4381 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg 4441 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg 4501 ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 4561 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 4621 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 4681 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 4741 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 4801 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 4861 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 4921 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 4981 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 5041 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 5101 gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact 5161 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5221 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5281 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 5341 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 5401 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 5461 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 5521 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 5581 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 5641 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 5701 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 5761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 5821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 5881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 5941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 6001 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 6061 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat 6121 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 6181 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 6241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 6301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 6361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 6421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 6481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 6541 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc 6601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 6661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 6721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 6781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 6841 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 6901 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 6961 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 7021 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 7081 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 7141 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 7201 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 7261 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 7321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 7381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 7441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 7501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 7561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 7621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 7681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 7741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 7801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 7861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 7921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 7981 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 8041 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 8101 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 8161 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag 8221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 8281 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc 8341 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 8401 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 8461 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt 8521 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 8581 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 8641 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 8701 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag 8761 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa 8821 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt 8881 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca 8941 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga 9001 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg 9061 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct 9121 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct 9181 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 9241 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 9301 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 9361 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg 9421 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag 9481 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag 9541 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa 9601 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt 9661 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc 9721 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta 9781 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga 9841 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct 9901 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 9961 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 10021 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 10081 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 10141 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 10201 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 10261 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 10321 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 10381 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 10441 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 10501 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 10561 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 10621 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 10681 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 10741 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaa //