LOCUS       Exported               12541 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of ERBB3 V104M.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-ERBB3-V104M
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7478..7502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7555..7596
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7617..8184
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7634..8186
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7778..7795)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8005..8024
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8198..8917
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8198..8914
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8243..8264)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8504..8523)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8851..8872
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8931..9519
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8984..9004)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9402..9413)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9594..9827
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9946..9964)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10032..10136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10137..10997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10355..10374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11168..11756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11657..11676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11910..11927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12019..12038
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    12167..12188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12203..12233
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12241..12257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12246..12268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12265..12281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12265..12281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12331..12350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg agggcgaacg acgctctgca ggtgctgggc
     3481 ttgcttttca gcctggcccg gggctccgag gtgggcaact ctcaggcagt gtgtcctggg
     3541 actctgaatg gcctgagtgt gaccggcgat gctgagaacc aataccagac actgtacaag
     3601 ctctacgaga ggtgtgaggt ggtgatgggg aaccttgaga ttgtgctcac gggacacaat
     3661 gccgacctct ccttcctgca gtggattcga gaagtgacag gctatgtcct cgtggccatg
     3721 aatgaattct ctactctacc attgcccaac ctccgcatgg tgcgagggac ccaggtctac
     3781 gatgggaagt ttgccatctt cgtcatgttg aactataaca ccaactccag ccacgctctg
     3841 cgccagctcc gcttgactca gctcaccgag attctgtcag ggggtgttta tattgagaag
     3901 aacgataagc tttgtcacat ggacacaatt gactggaggg acatcgtgag ggaccgagat
     3961 gctgagatag tggtgaagga caatggcaga agctgtcccc cctgtcatga ggtttgcaag
     4021 gggcgatgct ggggtcctgg atcagaagac tgccagacat tgaccaagac catctgtgct
     4081 cctcagtgta atggtcactg ctttgggccc aaccccaacc agtgctgcca tgatgagtgt
     4141 gccgggggct gctcaggccc tcaggacaca gactgctttg cctgccggca cttcaatgac
     4201 agtggagcct gtgtacctcg ctgtccacag cctcttgtct acaacaagct aactttccag
     4261 ctggaaccca atccccacac caagtatcag tatggaggag tttgtgtagc cagctgtccc
     4321 cataactttg tggtggatca aacatcctgt gtcagggcct gtcctcctga caagatggaa
     4381 gtagataaaa atgggctcaa gatgtgtgag ccttgtgggg gactatgtcc caaagcctgt
     4441 gagggaacag gctctgggag ccgcttccag actgtggact cgagcaacat tgatggattt
     4501 gtgaactgca ccaagatcct gggcaacctg gactttctga tcaccggcct caatggagac
     4561 ccctggcaca agatccctgc cctggaccca gagaagctca atgtcttccg gacagtacgg
     4621 gagatcacag gttacctgaa catccagtcc tggccgcccc acatgcacaa cttcagtgtt
     4681 ttttccaatt tgacaaccat tggaggcaga agcctctaca accggggctt ctcattgttg
     4741 atcatgaaga acttgaatgt cacatctctg ggcttccgat ccctgaagga aattagtgct
     4801 gggcgtatct atataagtgc caataggcag ctctgctacc accactcttt gaactggacc
     4861 aaggtgcttc gggggcctac ggaagagcga ctagacatca agcataatcg gccgcgcaga
     4921 gactgcgtgg cagagggcaa agtgtgtgac ccactgtgct cctctggggg atgctggggc
     4981 ccaggccctg gtcagtgctt gtcctgtcga aattatagcc gaggaggtgt ctgtgtgacc
     5041 cactgcaact ttctgaatgg ggagcctcga gaatttgccc atgaggccga atgcttctcc
     5101 tgccacccgg aatgccaacc catggagggc actgccacat gcaatggctc gggctctgat
     5161 acttgtgctc aatgtgccca ttttcgagat gggccccact gtgtgagcag ctgcccccat
     5221 ggagtcctag gtgccaaggg cccaatctac aagtacccag atgttcagaa tgaatgtcgg
     5281 ccctgccatg agaactgcac ccaggggtgt aaaggaccag agcttcaaga ctgtttagga
     5341 caaacactgg tgctgatcgg caaaacccat ctgacaatgg ctttgacagt gatagcagga
     5401 ttggtagtga ttttcatgat gctgggcggc acttttctct actggcgtgg gcgccggatt
     5461 cagaataaaa gggctatgag gcgatacttg gaacggggtg agagcataga gcctctggac
     5521 cccagtgaga aggctaacaa agtcttggcc agaatcttca aagagacaga gctaaggaag
     5581 cttaaagtgc ttggctcggg tgtctttgga actgtgcaca aaggagtgtg gatccctgag
     5641 ggtgaatcaa tcaagattcc agtctgcatt aaagtcattg aggacaagag tggacggcag
     5701 agttttcaag ctgtgacaga tcatatgctg gccattggca gcctggacca tgcccacatt
     5761 gtaaggctgc tgggactatg cccagggtca tctctgcagc ttgtcactca atatttgcct
     5821 ctgggttctc tgctggatca tgtgagacaa caccgggggg cactggggcc acagctgctg
     5881 ctcaactggg gagtacaaat tgccaaggga atgtactacc ttgaggaaca tggtatggtg
     5941 catagaaacc tggctgcccg aaacgtgcta ctcaagtcac ccagtcaggt tcaggtggca
     6001 gattttggtg tggctgacct gctgcctcct gatgataagc agctgctata cagtgaggcc
     6061 aagactccaa ttaagtggat ggcccttgag agtatccact ttgggaaata cacacaccag
     6121 agtgatgtct ggagctatgg tgtgacagtt tgggagttga tgaccttcgg ggcagagccc
     6181 tatgcagggc tacgattggc tgaagtacca gacctgctag agaaggggga gcggttggca
     6241 cagccccaga tctgcacaat tgatgtctac atggtgatgg tcaagtgttg gatgattgat
     6301 gagaacattc gcccaacctt taaagaacta gccaatgagt tcaccaggat ggcccgagac
     6361 ccaccacggt atctggtcat aaagagagag agtgggcctg gaatagcccc tgggccagag
     6421 ccccatggtc tgacaaacaa gaagctagag gaagtagagc tggagccaga actagaccta
     6481 gacctagact tggaagcaga ggaggacaac ctggcaacca ccacactggg ctccgccctc
     6541 agcctaccag ttggaacact taatcggcca cgtgggagcc agagcctttt aagtccatca
     6601 tctggataca tgcccatgaa ccagggtaat cttggggagt cttgccagga gtctgcagtt
     6661 tctgggagca gtgaacggtg cccccgtcca gtctctctac acccaatgcc acggggatgc
     6721 ctggcatcag agtcatcaga ggggcatgta acaggctctg aggctgagct ccaggagaaa
     6781 gtgtcaatgt gtaggagccg gagcaggagc cggagcccac ggccacgcgg agatagcgcc
     6841 taccattccc agcgccacag tctgctgact cctgttaccc cactctcccc acccgggtta
     6901 gaggaagagg atgtcaacgg ttatgtcatg ccagatacac acctcaaagg tactccctcc
     6961 tcccgggaag gcaccctttc ttcagtgggt ctcagttctg tcctgggtac tgaagaagaa
     7021 gatgaagatg aggagtatga atacatgaac cggaggagaa ggcacagtcc acctcatccc
     7081 cctaggccaa gttcccttga ggagctgggt tatgagtaca tggatgtggg gtcagacctc
     7141 agtgcctctc tgggcagcac acagagttgc ccactccacc ctgtacccat catgcccact
     7201 gcaggcacaa ctccagatga agactatgaa tatatgaatc ggcaacgaga tggaggtggt
     7261 cctgggggtg attatgcagc catgggggcc tgcccagcat ctgagcaagg gtatgaagag
     7321 atgagagctt ttcaggggcc tggacatcag gccccccatg tccattatgc ccgcctaaaa
     7381 actctacgta gcttagaggc tacagactct gcctttgata accctgatta ctggcatagc
     7441 aggcttttcc ccaaggctaa tgcccagaga acgtagaacc cagctttctt gtacaaagtg
     7501 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     7561 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     7621 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     7681 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     7741 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7801 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7861 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7921 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7981 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     8041 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     8101 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     8161 ttcctttgaa aaacacgatg ataatatggc cacacatatg gtgagcaagg gcgaggagct
     8221 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     8281 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     8341 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     8401 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8461 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8521 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8581 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     8641 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     8701 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8761 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     8821 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     8881 cgggatcact ctcggcatgg acgagctgta caagtaaatc gatagatcct aatcaacctc
     8941 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     9001 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     9061 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     9121 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     9181 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     9241 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     9301 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     9361 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     9421 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     9481 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     9541 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     9601 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     9661 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9721 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9781 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9841 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     9901 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     9961 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
    10021 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10081 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10141 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10201 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10261 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10321 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
    10381 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10441 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10501 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10561 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10621 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10681 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10741 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10801 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10861 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10921 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10981 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11041 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11101 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11161 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11221 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11281 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11341 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11401 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11461 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11521 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11581 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11641 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11701 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11761 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11821 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11881 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11941 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
    12001 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    12061 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
    12121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    12181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    12241 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    12301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    12361 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    12421 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    12481 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    12541 t
//
