LOCUS Exported 12128 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of EGFR M766_A767insAI. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-M766_A767insAI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12128) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12128) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12128 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 274..391 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 439..1617 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 670..1608 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1565..1585 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1637..1661 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5304..5328) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5336..5354 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5379..5398) /label=T7 /note="T7 promoter, forward primer" promoter complement(5380..5398) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5404..5424) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5478..5519 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5540..6107 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5557..6109 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5701..5718) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5928..5947 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6121..6720 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6121..6140) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6617..6637 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6734..7322 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6787..6807) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7205..7216) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7397..7630 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7749..7767) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7835..7939 /gene="bla" /label=AmpR promoter CDS 7940..8800 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8158..8177) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8971..9559 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9460..9479 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9713..9730 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9822..9841 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9970..9991 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10006..10036 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10044..10060 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10049..10071 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10068..10084 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10068..10084 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10134..10153 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10345..10978 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 11025..11150 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11647..11880 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12065..12109 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 61 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 121 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 181 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc 241 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg 301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt 361 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc 421 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 481 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 541 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 601 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 661 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 721 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt 781 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 841 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 901 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 961 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 1021 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 1081 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 1141 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 1201 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 1261 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 1321 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 1381 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 1441 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 1501 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 1561 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc 1621 ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc accatgcgac cctccgggac 1681 ggccggggca gcgctcctgg cgctgctggc tgcgctctgc ccggcgagtc gggctctgga 1741 ggaaaagaaa gtttgccaag gcacgagtaa caagctcacg cagttgggca cttttgaaga 1801 tcattttctc agcctccaga ggatgttcaa taactgtgag gtggtccttg ggaatttgga 1861 aattacctat gtgcagagga attatgatct ttccttctta aagaccatcc aggaggtggc 1921 tggttatgtc ctcattgccc tcaacacagt ggagcgaatt cctttggaaa acctgcagat 1981 catcagagga aatatgtact acgaaaattc ctatgcctta gcagtcttat ctaactatga 2041 tgcaaataaa accggactga aggagctgcc catgagaaat ttacaggaaa tcctgcatgg 2101 cgccgtgcgg ttcagcaaca accctgccct gtgcaacgtg gagagcatcc agtggcggga 2161 catagtcagc agtgactttc tcagcaacat gtcgatggac ttccagaacc acctgggcag 2221 ctgccaaaag tgtgatccaa gctgtcccaa tgggagctgc tggggtgcag gagaggagaa 2281 ctgccagaaa ctgaccaaaa tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa 2341 gtcccccagt gactgctgcc acaaccagtg tgctgcaggc tgcacaggcc cccgggagag 2401 cgactgcctg gtctgccgca aattccgaga cgaagccacg tgcaaggaca cctgcccccc 2461 actcatgctc tacaacccca ccacgtacca gatggatgtg aaccccgagg gcaaatacag 2521 ctttggtgcc acctgcgtga agaagtgtcc ccgtaattat gtggtgacag atcacggctc 2581 gtgcgtccga gcctgtgggg ccgacagcta tgagatggag gaagacggcg tccgcaagtg 2641 taagaagtgc gaagggcctt gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa 2701 agactcactc tccataaatg ctacgaatat taaacacttc aaaaactgca cctccatcag 2761 tggcgatctc cacatcctgc cggtggcatt taggggtgac tccttcacac atactcctcc 2821 tctggatcca caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct 2881 gattcaggct tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat 2941 acgcggcagg accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac 3001 atccttggga ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa 3061 caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca 3121 gaaaaccaaa attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg 3181 ccatgccttg tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg 3241 ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc 3301 aagggagttt gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc 3361 catgaacatc acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat 3421 tgacggcccc cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct 3481 ggtctggaag tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta 3541 cggatgcact gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat 3601 cgccactggg atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct 3661 cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag 3721 ggagcttgtg gagcctctta cacccagtgg agaagctccc aaccaagctc tcttgaggat 3781 cttgaaggaa actgaattca aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt 3841 gtataaggga ctctggatcc cagaaggtga gaaagttaaa attcccgtcg ctatcaagga 3901 attaagagaa gcaacatctc cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat 3961 ggcaatcgcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct gcctcacctc 4021 caccgtgcag ctcatcacgc agctcatgcc cttcggctgc ctcctggact atgtccggga 4081 acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga tcgcaaaggg 4141 catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca ggaacgtact 4201 ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac tgctgggtgc 4261 ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga tggcattgga 4321 atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg gggtgaccgt 4381 ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca gcgagatctc 4441 ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca tcgatgtcta 4501 catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt tccgtgagtt 4561 gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca ttcaggggga 4621 tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc tgatggatga 4681 agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc agggcttctt 4741 cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa ccagcaacaa 4801 ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca aggaagacag 4861 cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca gcatagacga 4921 caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc ccgctggctc 4981 tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca gagacccaca 5041 ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca ctgtccagcc 5101 cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag gcagccacca 5161 aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag ccaagccaaa 5221 tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg cgccacaaag 5281 cagtgaattt attggagcat agaacccagc tttcttgtac aaagtggtga tatccaatta 5341 accctcacta aagggttatg atatagtgag ttgttattcc ctatagtgag tcgtattacc 5401 acccaacttt tctatacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag 5461 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc 5521 gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg 5581 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca 5641 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc 5701 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag 5761 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg 5821 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac 5881 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa 5941 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc 6001 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc 6061 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat 6121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctaa 6721 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 7021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 7081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 7141 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 7201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 7261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 7321 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 7381 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt 7441 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 7501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 7561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 7621 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 7681 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag 7741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 7801 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 7861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 7921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 7981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 8041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 8101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 8161 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 8221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 8281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 8341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 8401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 8461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 8521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 8581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 8641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 8701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 8761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 8821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 8881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 8941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 9001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 9061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 9121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 9181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 9241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 9301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 9361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 9421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 9481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 9541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 9601 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 9661 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 9721 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 9781 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag 9841 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 9901 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 9961 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 10021 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 10081 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 10141 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca 10201 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca 10261 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta 10321 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata 10381 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac 10441 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 10501 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga 10561 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc 10621 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc 10681 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga 10741 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg 10801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 10861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 10921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 10981 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 11041 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 11101 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 11161 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 11221 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 11281 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 11341 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 11401 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 11461 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg 11521 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc 11581 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt 11641 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc 11701 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa 11761 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat 11821 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct 11881 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag 11941 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag 12001 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca 12061 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt 12121 taacataa //