LOCUS Exported 10924 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of FGFR4 K157N. ACCESSION . VERSION . KEYWORDS pHAGE-FGFR4-K157N SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10924) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10924) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10924 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 20..44 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1218..1229 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind complement(2460..2484) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2537..2578 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2599..3166 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 2616..3168 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2760..2777) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2987..3006 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3180..3899 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3180..3896 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3225..3246) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3486..3505) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3833..3854 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3913..4501 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3966..3986) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4384..4395) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4576..4809 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(4928..4946) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5014..5118 /gene="bla" /label=AmpR promoter CDS 5119..5979 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5337..5356) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6150..6738 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6639..6658 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6892..6909 /label=L4440 /note="L4440 vector, forward primer" primer_bind 7001..7020 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 7149..7170 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7185..7215 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7223..7239 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7228..7250 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7247..7263 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7247..7263 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7313..7332 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 7524..8157 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 8204..8329 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8826..9059 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9244..9288 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9581..9698 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 9746..10924 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 9977..10915 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 10872..10892 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggctccacca tgcggctgct 61 gctggccctg ttgggggtcc tgctgagtgt gcctgggcct ccagtcttgt ccctggaggc 121 ctctgaggaa gtggagcttg agccctgcct ggctcccagc ctggagcagc aagagcagga 181 gctgacagta gcccttgggc agcctgtgcg tctgtgctgt gggcgggctg agcgtggtgg 241 ccactggtac aaggagggca gtcgcctggc acctgctggc cgtgtacggg gctggagggg 301 ccgcctagag attgccagct tcctacctga ggatgctggc cgctacctct gcctggcacg 361 aggctccatg atcgtcctgc agaatctcac cttgattaca ggtgactcct tgacctccag 421 caacgatgat gaggacccca agtcccatag ggacccctcg aataggcaca gttaccccca 481 gcaagcaccc tactggacac acccccagcg catggagaac aaactgcatg cagtacctgc 541 ggggaacacc gtcaagttcc gctgtccagc tgcaggcaac cccacgccca ccatccgctg 601 gcttaaggat ggacaggcct ttcatgggga gaaccgcatt ggaggcattc ggctgcgcca 661 tcagcactgg agtctcgtga tggagagcgt ggtgccctcg gaccgcggca catacacctg 721 cctggtagag aacgctgtgg gcagcatccg ctataactac ctgctagatg tgctggagcg 781 gtccccgcac cggcccatcc tgcaggccgg gctcccggcc aacaccacag ccgtggtggg 841 cagcgacgtg gagctgctgt gcaaggtgta cagcgatgcc cagccccaca tccagtggct 901 gaagcacatc gtcatcaacg gcagcagctt cggagccgac ggtttcccct atgtgcaagt 961 cctaaagact gcagacatca atagctcaga ggtggaggtc ctgtacctgc ggaacgtgtc 1021 agccgaggac gcaggcgagt acacctgcct cgcaggcaat tccatcggcc tctcctacca 1081 gtctgcctgg ctcacggtgc tgccagagga ggaccccaca tggaccgcag cagcgcccga 1141 ggccaggtat acggacatca tcctgtacgc gtcgggctcc ctggccttgg ctgtgctcct 1201 gctgctggcc gggctgtatc gagggcaggc gctccacggc cggcaccccc gcccgcccgc 1261 cactgtgcag aagctctccc gcttccctct ggcccgacag ttctccctgg agtcaggctc 1321 ttccggcaag tcaagctcat ccctggtacg aggcgtgcgt ctctcctcca gcggccccgc 1381 cttgctcgcc ggcctcgtga gtctagatct acctctcgac ccactatggg agttcccccg 1441 ggacaggctg gtgcttggga agcccctagg cgagggctgc tttggccagg tagtacgtgc 1501 agaggccttt ggcatggacc ctgcccggcc tgaccaagcc agcactgtgg ccgtcaagat 1561 gctcaaagac aacgcctctg acaaggacct ggccgacctg gtctcggaga tggaggtgat 1621 gaagctgatc ggccgacaca agaacatcat caacctgctt ggtgtctgca cccaggaagg 1681 gcccctgtac gtgatcgtgg agtgcgccgc caagggaaac ctgcgggagt tcctgcgggc 1741 ccggcgcccc ccaggccccg acctcagccc cgacggtcct cggagcagtg aggggccgct 1801 ctccttccca gtcctggtct cctgcgccta ccaggtggcc cgaggcatgc agtatctgga 1861 gtcccggaag tgtatccacc gggacctggc tgcccgcaat gtgctggtga ctgaggacaa 1921 tgtgatgaag attgctgact ttgggctggc ccgcggcgtc caccacattg actactataa 1981 gaaaaccagc aacggccgcc tgcctgtgaa gtggatggcg cccgaggcct tgtttgaccg 2041 ggtgtacaca caccagagtg acgtgtggtc ttttgggatc ctgctatggg agatcttcac 2101 cctcgggggc tccccgtatc ctggcatccc ggtggaggag ctgttctcgc tgctgcggga 2161 gggacatcgg atggaccgac ccccacactg ccccccagag ctgtacgggc tgatgcgtga 2221 gtgctggcac gcagcgccct cccagaggcc taccttcaag cagctggtgg aggcgctgga 2281 caaggtcctg ctggccgtct ctgaggagta cctcgacctc cgcctgacct tcggacccta 2341 ttccccctct ggtggggacg ccagcagcac ctgctcctcc agcgattctg tcttcagcca 2401 cgaccccctg ccattgggat ccagctcctt ccccttcggg tctggggtgc agacatagga 2461 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga 2521 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg 2581 taccggttag taatgagatc cctccccccc ccctaacgtt actggccgaa gccgcttgga 2641 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 2701 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 2761 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 2821 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 2881 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 2941 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 3001 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 3061 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 3121 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata 3181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 3241 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 3301 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 3361 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 3421 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 3481 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 3541 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 3601 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 3661 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 3721 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 3781 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 3841 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa 3901 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 3961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 4021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 4081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 4141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 4201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 4261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 4321 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 4381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 4441 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 4501 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 4561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 4621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 4681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 4741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 4801 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 4861 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga 4921 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 4981 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 5041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 5101 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 5161 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 5221 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 5281 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 5341 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac 5401 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 5461 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 5521 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 5581 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 5641 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 5701 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 5761 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 5821 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 5881 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 5941 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 6001 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 6061 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 6121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 6181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 6241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 6301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 6361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 6421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 6481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 6541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 6601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 6661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 6721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 6781 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 6841 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 6901 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 6961 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg 7021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 7081 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 7141 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 7201 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 7261 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7321 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa 7381 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat 7441 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa 7501 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat 7561 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc 7621 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga 7681 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag 7741 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct 7801 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg 7861 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac 7921 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt 7981 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 8041 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 8101 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg 8161 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac 8221 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa 8281 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg 8341 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata 8401 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg 8461 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca 8521 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca 8581 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa 8641 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga 8701 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 8761 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 8821 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 8881 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 8941 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 9001 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 9061 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 9121 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 9181 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 9241 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 9301 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 9361 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 9421 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 9481 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 9541 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 9601 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 9661 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 9721 gagatccagt ttggactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc 9781 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag 9841 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 9901 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 9961 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg 10021 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc 10081 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc 10141 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg 10201 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg 10261 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca 10321 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac 10381 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca 10441 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc 10501 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc 10561 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc 10621 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta 10681 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg 10741 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt 10801 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc 10861 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc 10921 gtga //