LOCUS Exported 12857 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PTCH1 T768I. ACCESSION . VERSION . KEYWORDS pHAGE-PTCH1-T768I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12857) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12857) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12857 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7817..7841) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7849..7867 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7892..7911) /label=T7 /note="T7 promoter, forward primer" promoter complement(7893..7911) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(7917..7937) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 7991..8032 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 8053..8620 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 8070..8622 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(8214..8231) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 8441..8460 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8634..9233 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8634..8653) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9130..9150 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9247..9835 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9300..9320) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9718..9729) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9910..10143 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(10262..10280) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10348..10452 /gene="bla" /label=AmpR promoter CDS 10453..11313 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10671..10690) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11484..12072 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11973..11992 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12226..12243 /label=L4440 /note="L4440 vector, forward primer" primer_bind 12335..12354 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 12483..12504 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12519..12549 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12557..12573 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12562..12584 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12581..12597 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12581..12597 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12647..12666 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatggcctc ggctggtaac 3481 gccgccgagc cccaggaccg cggcggcggc ggcagcggct gtatcggtgc cccgggacgg 3541 ccggctggag gcgggaggcg cagacggacg ggggggctgc gccgtgctgc cgcgccggac 3601 cgggactatc tgcaccggcc cagctactgc gacgccgcct tcgctctgga gcagatttcc 3661 aaggggaagg ctactggccg gaaagcgccg ctgtggctga gagcgaagtt tcagagactc 3721 ttatttaaac tgggttgtta cattcaaaaa aactgcggca agttcttggt tgtgggcctc 3781 ctcatatttg gggccttcgc ggtgggatta aaagcagcga acctcgagac caacgtggag 3841 gagctgtggg tggaagttgg aggacgagta agtcgtgaat taaattatac tcgccagaag 3901 attggagaag aggctatgtt taatcctcaa ctcatgatac agacccctaa agaagaaggt 3961 gctaatgtcc tgaccacaga agcgctccta caacacctgg actcggcact ccaggccagc 4021 cgtgtccatg tatacatgta caacaggcag tggaaattgg aacatttgtg ttacaaatca 4081 ggagagctta tcacagaaac aggttacatg gatcagataa tagaatatct ttacccttgt 4141 ttgattatta cacctttgga ctgcttctgg gaaggggcga aattacagtc tgggacagca 4201 tacctcctag gtaaacctcc tttgcggtgg acaaacttcg accctttgga attcctggaa 4261 gagttaaaga aaataaacta tcaagtggac agctgggagg aaatgctgaa taaggctgag 4321 gttggtcatg gttacatgga ccgcccctgc ctcaatccgg ccgatccaga ctgccccgcc 4381 acagccccca acaaaaattc aaccaaacct cttgatatgg cccttgtttt gaatggtgga 4441 tgtcatggct tatccagaaa gtatatgcac tggcaggagg agttgattgt gggtggcaca 4501 gtcaagaaca gcactggaaa actcgtcagc gcccatgccc tgcagaccat gttccagtta 4561 atgactccca agcaaatgta cgagcacttc aaggggtacg agtatgtctc acacatcaac 4621 tggaacgagg acaaagcggc agccatcctg gaggcctggc agaggacata tgtggaggtg 4681 gttcatcaga gtgtcgcaca gaactccact caaaaggtgc tttccttcac caccacgacc 4741 ctggacgaca tcctgaaatc cttctctgac gtcagtgtca tccgcgtggc cagcggctac 4801 ttactcatgc tcgcctatgc ctgtctaacc atgctgcgct gggactgctc caagtcccag 4861 ggtgccgtgg ggctggctgg cgtcctgctg gttgcactgt cagtggctgc aggactgggc 4921 ctgtgctcat tgatcggaat ttcctttaac gctgcaacaa ctcaggtttt gccatttctc 4981 gctcttggtg ttggtgtgga tgatgttttt cttctggccc acgccttcag tgaaacagga 5041 cagaataaaa gaatcccttt tgaggacagg accggggagt gcctgaagcg cacaggagcc 5101 agcgtggccc tcacgtccat cagcaatgtc acagccttct tcatggccgc gttaatccca 5161 attcccgctc tgcgggcgtt ctccctccag gcagcggtag tagtggtgtt caattttgcc 5221 atggttctgc tcatttttcc tgcaattctc agcatggatt tatatcgacg cgaggacagg 5281 agactggata ttttctgctg ttttacaagc ccctgcgtca gcagagtgat tcaggttgaa 5341 cctcaggcct acaccgacac acacgacaat acccgctaca gccccccacc tccctacagc 5401 agccacagct ttgcccatga aacgcagatt accatgcagt ccactgtcca gctccgcacg 5461 gagtacgacc cccacacgca cgtgtactac accaccgctg agccgcgctc cgagatctct 5521 gtgcagcccg tcaccgtgac acaggacacc ctcagctgcc agagcccaga gagcaccagc 5581 tccacaaggg acctgctctc ccagttctcc gactccagcc tccactgcct cgagcccccc 5641 tgtacgaagt ggacactctc atcttttgct gagaagcact atgctccttt cctcttgaaa 5701 ccaaaagcca aggtagtggt gatcttcctt tttctgggct tgctgggggt cagcctttat 5761 ggcatcaccc gagtgagaga cgggctggac cttacggaca ttgtacctcg ggaaaccaga 5821 gaatatgact ttattgctgc acaattcaaa tacttttctt tctacaacat gtatatagtc 5881 acccagaaag cagactaccc gaatatccag cacttacttt acgacctaca caggagtttc 5941 agtaacgtga agtatgtcat gttggaagaa aacaaacagc ttcccaaaat gtggctgcac 6001 tacttcagag actggcttca gggacttcag gatgcatttg acagtgactg ggaaaccggg 6061 aaaatcatgc caaacaatta caagaatgga tcagacgatg gagtccttgc ctacaaactc 6121 ctggtgcaaa ccggcagccg cgataagccc atcgacatca gccagttgac taaacagcgt 6181 ctggtggatg cagatggcat cattaatccc agcgctttct acatctacct gacggcttgg 6241 gtcagcaacg accccgtcgc gtatgctgcc tcccaggcca acatccggcc acaccgacca 6301 gaatgggtcc acgacaaagc cgactacatg cctgaaacaa ggctgagaat cccggcagca 6361 gagcccatcg agtatgccca gttccctttc tacctcaacg gcttgcggga cacctcagac 6421 tttgtggagg caattgaaaa agtaaggacc atctgcagca actatacgag cctggggctg 6481 tccagttacc ccaacggcta ccccttcctc ttctgggagc agtacatcgg cctccgccac 6541 tggctgctgc tgttcatcag cgtggtgttg gcctgcacat tcctcgtgtg cgctgtcttc 6601 cttctgaacc cctggacggc cgggatcatt gtgatggtcc tggcgctgat gacggtcgag 6661 ctgttcggca tgatgggcct catcggaatc aagctcagtg ccgtgcccgt ggtcatcctg 6721 atcgcttctg ttggcatagg agtggagttc accgttcacg ttgctttggc ctttctgacg 6781 gccatcggcg acaagaaccg cagggctgtg cttgccctgg agcacatgtt tgcacccgtc 6841 ctggatggcg ccgtgtccac tctgctggga gtgctgatgc tggcgggatc tgagttcgac 6901 ttcattgtca ggtatttctt tgctgtgctg gcgatcctca ccatcctcgg cgttctcaat 6961 gggctggttt tgcttcccgt gcttttgtct ttctttggac catatcctga ggtgtctcca 7021 gccaacggct tgaaccgcct gcccacaccc tcccctgagc caccccccag cgtggtccgc 7081 ttcgccatgc cgcccggcca cacgcacagc gggtctgatt cctccgactc ggagtatagt 7141 tcccagacga cagtgtcagg cctcagcgag gagcttcggc actacgaggc ccagcagggc 7201 gcgggaggcc ctgcccacca agtgatcgtg gaagccacag aaaaccccgt cttcgcccac 7261 tccactgtgg tccatcccga atccaggcat cacccaccct cgaacccgag acagcagccc 7321 cacctggact cagggtccct gcctcccgga cggcaaggcc agcagccccg cagggacccc 7381 cccagagaag gcttgtggcc acccccctac agaccgcgca gagacgcttt tgaaatttct 7441 actgaagggc attctggccc tagcaatagg gcccgctggg gccctcgcgg ggcccgttct 7501 cacaaccctc ggaacccagc gtccactgcc atgggcagct ccgtgcccgg ctactgccag 7561 cccatcacca ctgtgacggc ttctgcctcc gtgactgtcg ccgtgcaccc gccgcctgtc 7621 cctgggcctg ggcggaaccc ccgaggggga ctctgcccag gctaccctga gactgaccac 7681 ggcctgtttg aggaccccca cgtgcctttc cacgtccggt gtgagaggag ggattcgaag 7741 gtggaagtca ttgagctgca ggacgtggaa tgcgaggaga ggccccgggg aagcagctcc 7801 aactcaggcc tcatgggccc agctttcttg tacaaagtgg tgatatccaa ttaaccctca 7861 ctaaagggtt atgaaagagt atgtgttaaa tccctatagt gagtcgtatt accacccaac 7921 ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc 7981 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg 8041 ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 8101 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 8161 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 8221 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 8281 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 8341 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 8401 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 8461 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 8521 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 8581 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg 8641 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 8701 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 8761 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 8821 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 8881 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 8941 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 9001 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 9061 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 9121 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 9181 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata 9241 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 9301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 9361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 9421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 9481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 9541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 9601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 9661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 9721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 9781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag 9841 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 9901 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 9961 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 10021 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 10081 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 10141 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 10201 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag 10261 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 10321 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 10381 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 10441 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 10501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 10561 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 10621 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 10681 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct 10741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 10801 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 10861 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 10921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 10981 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 11041 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 11101 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 11161 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 11221 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 11281 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 11341 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 11401 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 11461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 11521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 11581 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 11641 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 11701 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 11761 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 11821 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 11881 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 11941 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 12001 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 12061 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 12121 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 12181 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 12241 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 12301 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 12361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 12421 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 12481 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 12541 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg 12601 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 12661 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 12721 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 12781 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 12841 actacaaact tagtagt //