LOCUS       Exported               12529 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of FLT1 L778P.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-FLT1-L778P
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5409..5433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5486..5527
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5548..6115
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5565..6117
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5709..5726)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5936..5955
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6129..6848
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6129..6845
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6174..6195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6435..6454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6782..6803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6862..7450
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6915..6935)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7333..7344)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7525..7758
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7877..7895)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7963..8067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8068..8928
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8286..8305)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9099..9687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9588..9607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9841..9858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9950..9969
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    10098..10119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10134..10164
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10172..10188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10177..10199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10196..10212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10196..10212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10262..10281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10473..11106
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11153..11278
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11775..12008
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12193..12237
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggtcagct actgggacac cggggtcctg ctgtgcgcgc tgctcagctg
     1441 tctgcttctc acaggatcta gttcaggttc aaaattaaaa gatcctgaac tgagtttaaa
     1501 aggcacccag cacatcatgc aagcaggcca gacactgcat ctccaatgca ggggggaagc
     1561 agcccataaa tggtctttgc ctgaaatggt gagtaaggaa agcgaaaggc tgagcataac
     1621 taaatctgcc tgtggaagaa atggcaaaca attctgcagt actttaacct tgaacacagc
     1681 tcaagcaaac cacactggct tctacagctg caaatatcta gctgtaccta cttcaaagaa
     1741 gaaggaaaca gaatctgcaa tctatatatt tattagtgat acaggtagac ctttcgtaga
     1801 gatgtacagt gaaatccccg aaattataca catgactgaa ggaagggagc tcgtcattcc
     1861 ctgccgggtt acgtcaccta acatcactgt tactttaaaa aagtttccac ttgacacttt
     1921 gatccctgat ggaaaacgca taatctggga cagtagaaag ggcttcatca tatcaaatgc
     1981 aacgtacaaa gaaatagggc ttctgacctg tgaagcaaca gtcaatgggc atttgtataa
     2041 gacaaactat ctcacacatc gacaaaccaa tacaatcata gatgtccaaa taagcacacc
     2101 acgcccagtc aaattactta gaggccatac tcttgtcctc aattgtactg ctaccactcc
     2161 cttgaacacg agagttcaaa tgacctggag ttaccctgat gaaaaaaata agagagcttc
     2221 cgtaaggcga cgaattgacc aaagcaattc ccatgccaac atattctaca gtgttcttac
     2281 tattgacaaa atgcagaaca aagacaaagg actttatact tgtcgtgtaa ggagtggacc
     2341 atcattcaaa tctgttaaca cctcagtgca tatatatgat aaagcattca tcactgtgaa
     2401 acatcgaaaa cagcaggtgc ttgaaaccgt agctggcaag cggtcttacc ggctctctat
     2461 gaaagtgaag gcatttccct cgccggaagt tgtatggtta aaagatgggt tacctgcgac
     2521 tgagaaatct gctcgctatt tgactcgtgg ctactcgtta attatcaagg acgtaactga
     2581 agaggatgca gggaattata caatcttgct gagcataaaa cagtcaaatg tgtttaaaaa
     2641 cctcactgcc actctaattg tcaatgtgaa accccagatt tacgaaaagg ccgtgtcatc
     2701 gtttccagac ccggctctct acccactggg cagcagacaa atcctgactt gtaccgcata
     2761 tggtatccct caacctacaa tcaagtggtt ctggcacccc tgtaaccata atcattccga
     2821 agcaaggtgt gacttttgtt ccaataatga agagtccttt atcctggatg ctgacagcaa
     2881 catgggaaac agaattgaga gcatcactca gcgcatggca ataatagaag gaaagaataa
     2941 gatggctagc accttggttg tggctgactc tagaatttct ggaatctaca tttgcatagc
     3001 ttccaataaa gttgggactg tgggaagaaa cataagcttt tatatcacag atgtgccaaa
     3061 tgggtttcat gttaacttgg aaaaaatgcc gacggaagga gaggacctga aactgtcttg
     3121 cacagttaac aagttcttat acagagacgt tacttggatt ttactgcgga cagttaataa
     3181 cagaacaatg cactacagta ttagcaagca aaaaatggcc atcactaagg agcactccat
     3241 cactcttaat cttaccatca tgaatgtttc cctgcaagat tcaggcacct atgcctgcag
     3301 agccaggaat gtatacacag gggaagaaat cctccagaag aaagaaatta caatcagaga
     3361 tcaggaagca ccatacctcc tgcgaaacct cagtgatcac acagtggcca tcagcagttc
     3421 caccacttta gactgtcatg ctaatggtgt ccccgagcct cagatcactt ggtttaaaaa
     3481 caaccacaaa atacaacaag agcctggaat tattttagga ccaggaagca gcacgctgtt
     3541 tattgaaaga gtcacagaag aggatgaagg tgtctatcac tgcaaagcca ccaaccagaa
     3601 gggctctgtg gaaagttcag catacctcac tgttcaagga acctcggaca agtctaatct
     3661 ggagctgatc actctaacat gcacctgtgt ggctgcgact ctcttctggc tcctattaac
     3721 cccctttatc cgaaaaatga aaaggtcttc ttctgaaata aagactgact acctatcaat
     3781 tataatggac ccagatgaag ttcctttgga tgagcagtgt gagcggctcc cttatgatgc
     3841 cagcaagtgg gagtttgccc gggagagact taaactgggc aaatcacttg gaagaggggc
     3901 ttttggaaaa gtggttcaag catcagcatt tggcattaag aaatcaccta cgtgccggac
     3961 tgtggctgtg aaaatgctga aagagggggc cacggccagc gagtacaaag ctctgatgac
     4021 tgagctaaaa atcttgaccc acattggcca ccatctgaac gtggttaacc tgctgggagc
     4081 ctgcaccaag caaggagggc ctctgatggt gattgttgaa tactgcaaat atggaaatct
     4141 ctccaactac ctcaagagca aacgtgactt attttttctc aacaaggatg cagcactaca
     4201 catggagcct aagaaagaaa aaatggagcc aggcctggaa caaggcaaga aaccaagact
     4261 agatagcgtc accagcagcg aaagctttgc gagctccggc tttcaggaag ataaaagtct
     4321 gagtgatgtt gaggaagagg aggattctga cggtttctac aaggagccca tcactatgga
     4381 agatctgatt tcttacagtt ttcaagtggc cagaggcatg gagttcctgt cttccagaaa
     4441 gtgcattcat cgggacctgg cagcgagaaa cattctttta tctgagaaca acgtggtgaa
     4501 gatttgtgat tttggccttg cccgggatat ttataagaac cccgattatg tgagaaaagg
     4561 agatactcga cttcctctga aatggatggc tcctgaatct atctttgaca aaatctacag
     4621 caccaagagc gacgtgtggt cttacggagt attgctgtgg gaaatcttct ccttaggtgg
     4681 gtctccatac ccaggagtac aaatggatga ggacttttgc agtcgcctga gggaaggcat
     4741 gaggatgaga gctcctgagt actctactcc tgaaatctat cagatcatgc tggactgctg
     4801 gcacagagac ccaaaagaaa ggccaagatt tgcagaactt gtggaaaaac taggtgattt
     4861 gcttcaagca aatgtacaac aggatggtaa agactacatc ccaatcaatg ccatactgac
     4921 aggaaatagt gggtttacat actcaactcc tgccttctct gaggacttct tcaaggaaag
     4981 tatttcagct ccgaagttta attcaggaag ctctgatgat gtcagatacg taaatgcttt
     5041 caagttcatg agcctggaaa gaatcaaaac ctttgaagaa cttttaccga atgccacctc
     5101 catgtttgat gactaccagg gcgacagcag cactctgttg gcctctccca tgctgaagcg
     5161 cttcacctgg actgacagca aacccaaggc ctcgctcaag attgacttga gagtaaccag
     5221 taaaagtaag gagtcggggc tgtctgatgt cagcaggccc agtttctgcc attccagctg
     5281 tgggcacgtc agcgaaggca agcgcaggtt cacctacgac cacgctgagc tggaaaggaa
     5341 aatcgcgtgc tgctccccgc ccccagacta caactcggtg gtcctgtact ccaccccacc
     5401 catctagaac ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
     5461 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     5521 ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag
     5581 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5641 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5701 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     5761 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5821 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5881 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     5941 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6001 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6061 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     6121 ccacacatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     6181 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     6241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     6301 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     6361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     6421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     6481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     6541 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     6601 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     6661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6841 acaagtaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     6901 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6961 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7021 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7081 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7141 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7201 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7261 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7321 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7381 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7441 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7501 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7561 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7621 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7681 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7741 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7801 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     7861 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7921 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7981 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8041 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8101 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8161 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8221 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8281 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8341 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8401 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8461 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8521 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8581 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8641 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8701 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8761 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8821 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8881 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8941 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9001 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9241 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9481 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9721 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9781 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9841 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9901 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     9961 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10021 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
    10081 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10141 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10201 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
    10261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10321 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10381 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10441 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
    10501 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
    10561 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
    10621 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
    10681 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
    10741 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
    10801 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
    10861 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
    10921 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10981 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    11041 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    11101 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
    11161 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
    11221 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
    11281 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
    11341 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
    11401 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
    11461 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
    11521 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
    11581 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
    11641 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11701 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11761 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11821 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11881 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11941 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    12001 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    12061 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    12121 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    12181 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    12241 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    12301 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    12361 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    12421 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    12481 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaa
//
