LOCUS Exported 12506 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of FLT1 V395I. ACCESSION . VERSION . KEYWORDS pHAGE-FLT1-V395I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12506) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12506) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12506 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 20..44 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4065..4089) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4097..4115 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4140..4159) /label=T7 /note="T7 promoter, forward primer" promoter complement(4141..4159) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(4165..4185) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 4239..4280 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4301..4868 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4318..4870 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4462..4479) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4689..4708 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4882..5481 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4882..4901) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5378..5398 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5495..6083 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5548..5568) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5966..5977) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6158..6391 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6510..6528) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6596..6700 /gene="bla" /label=AmpR promoter CDS 6701..7561 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6919..6938) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7732..8320 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8221..8240 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8474..8491 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8583..8602 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8731..8752 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8767..8797 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8805..8821 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8810..8832 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8829..8845 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8829..8845 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8895..8914 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 9106..9739 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9786..9911 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10408..10641 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10826..10870 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 11163..11280 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 11328..12506 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 11559..12497 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 12454..12474 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg tcagctactg 61 ggacaccggg gtcctgctgt gcgcgctgct cagctgtctg cttctcacag gatctagttc 121 aggttcaaaa ttaaaagatc ctgaactgag tttaaaaggc acccagcaca tcatgcaagc 181 aggccagaca ctgcatctcc aatgcagggg ggaagcagcc cataaatggt ctttgcctga 241 aatggtgagt aaggaaagcg aaaggctgag cataactaaa tctgcctgtg gaagaaatgg 301 caaacaattc tgcagtactt taaccttgaa cacagctcaa gcaaaccaca ctggcttcta 361 cagctgcaaa tatctagctg tacctacttc aaagaagaag gaaacagaat ctgcaatcta 421 tatatttatt agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat 481 tatacacatg actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat 541 cactgttact ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat 601 ctgggacagt agaaagggct tcatcatatc aaatgcaacg tacaaagaaa tagggcttct 661 gacctgtgaa gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca 721 aaccaataca atcatagatg tccaaataag cacaccacgc ccagtcaaat tacttagagg 781 ccatactctt gtcctcaatt gtactgctac cactcccttg aacacgagag ttcaaatgac 841 ctggagttac cctgatgaaa aaaataagag agcttccgta aggcgacgaa ttgaccaaag 901 caattcccat gccaacatat tctacagtgt tcttactatt gacaaaatgc agaacaaaga 961 caaaggactt tatacttgtc gtgtaaggag tggaccatca ttcaaatctg ttaacacctc 1021 agtgcatata tatgataaag cattcatcac tgtgaaacat cgaaaacagc aggtgcttga 1081 aaccgtagct ggcaagcggt cttaccggct ctctatgaaa gtgaaggcat ttccctcgcc 1141 ggaagttgta tggttaaaag atgggttacc tgcgactgag aaatctgctc gctatttgac 1201 tcgtggctac tcgttaatta tcaaggacat aactgaagag gatgcaggga attatacaat 1261 cttgctgagc ataaaacagt caaatgtgtt taaaaacctc actgccactc taattgtcaa 1321 tgtgaaaccc cagatttacg aaaaggccgt gtcatcgttt ccagacccgg ctctctaccc 1381 actgggcagc agacaaatcc tgacttgtac cgcatatggt atccctcaac ctacaatcaa 1441 gtggttctgg cacccctgta accataatca ttccgaagca aggtgtgact tttgttccaa 1501 taatgaagag tcctttatcc tggatgctga cagcaacatg ggaaacagaa ttgagagcat 1561 cactcagcgc atggcaataa tagaaggaaa gaataagatg gctagcacct tggttgtggc 1621 tgactctaga atttctggaa tctacatttg catagcttcc aataaagttg ggactgtggg 1681 aagaaacata agcttttata tcacagatgt gccaaatggg tttcatgtta acttggaaaa 1741 aatgccgacg gaaggagagg acctgaaact gtcttgcaca gttaacaagt tcttatacag 1801 agacgttact tggattttac tgcggacagt taataacaga acaatgcact acagtattag 1861 caagcaaaaa atggccatca ctaaggagca ctccatcact cttaatctta ccatcatgaa 1921 tgtttccctg caagattcag gcacctatgc ctgcagagcc aggaatgtat acacagggga 1981 agaaatcctc cagaagaaag aaattacaat cagagatcag gaagcaccat acctcctgcg 2041 aaacctcagt gatcacacag tggccatcag cagttccacc actttagact gtcatgctaa 2101 tggtgtcccc gagcctcaga tcacttggtt taaaaacaac cacaaaatac aacaagagcc 2161 tggaattatt ttaggaccag gaagcagcac gctgtttatt gaaagagtca cagaagagga 2221 tgaaggtgtc tatcactgca aagccaccaa ccagaagggc tctgtggaaa gttcagcata 2281 cctcactgtt caaggaacct cggacaagtc taatctggag ctgatcactc taacatgcac 2341 ctgtgtggct gcgactctct tctggctcct attaaccctc tttatccgaa aaatgaaaag 2401 gtcttcttct gaaataaaga ctgactacct atcaattata atggacccag atgaagttcc 2461 tttggatgag cagtgtgagc ggctccctta tgatgccagc aagtgggagt ttgcccggga 2521 gagacttaaa ctgggcaaat cacttggaag aggggctttt ggaaaagtgg ttcaagcatc 2581 agcatttggc attaagaaat cacctacgtg ccggactgtg gctgtgaaaa tgctgaaaga 2641 gggggccacg gccagcgagt acaaagctct gatgactgag ctaaaaatct tgacccacat 2701 tggccaccat ctgaacgtgg ttaacctgct gggagcctgc accaagcaag gagggcctct 2761 gatggtgatt gttgaatact gcaaatatgg aaatctctcc aactacctca agagcaaacg 2821 tgacttattt tttctcaaca aggatgcagc actacacatg gagcctaaga aagaaaaaat 2881 ggagccaggc ctggaacaag gcaagaaacc aagactagat agcgtcacca gcagcgaaag 2941 ctttgcgagc tccggctttc aggaagataa aagtctgagt gatgttgagg aagaggagga 3001 ttctgacggt ttctacaagg agcccatcac tatggaagat ctgatttctt acagttttca 3061 agtggccaga ggcatggagt tcctgtcttc cagaaagtgc attcatcggg acctggcagc 3121 gagaaacatt cttttatctg agaacaacgt ggtgaagatt tgtgattttg gccttgcccg 3181 ggatatttat aagaaccccg attatgtgag aaaaggagat actcgacttc ctctgaaatg 3241 gatggctcct gaatctatct ttgacaaaat ctacagcacc aagagcgacg tgtggtctta 3301 cggagtattg ctgtgggaaa tcttctcctt aggtgggtct ccatacccag gagtacaaat 3361 ggatgaggac ttttgcagtc gcctgaggga aggcatgagg atgagagctc ctgagtactc 3421 tactcctgaa atctatcaga tcatgctgga ctgctggcac agagacccaa aagaaaggcc 3481 aagatttgca gaacttgtgg aaaaactagg tgatttgctt caagcaaatg tacaacagga 3541 tggtaaagac tacatcccaa tcaatgccat actgacagga aatagtgggt ttacatactc 3601 aactcctgcc ttctctgagg acttcttcaa ggaaagtatt tcagctccga agtttaattc 3661 aggaagctct gatgatgtca gatacgtaaa tgctttcaag ttcatgagcc tggaaagaat 3721 caaaaccttt gaagaacttt taccgaatgc cacctccatg tttgatgact accagggcga 3781 cagcagcact ctgttggcct ctcccatgct gaagcgcttc acctggactg acagcaaacc 3841 caaggcctcg ctcaagattg acttgagagt aaccagtaaa agtaaggagt cggggctgtc 3901 tgatgtcagc aggcccagtt tctgccattc cagctgtggg cacgtcagcg aaggcaagcg 3961 caggttcacc tacgaccacg ctgagctgga aaggaaaatc gcgtgctgct ccccgccccc 4021 agactacaac tcggtggtcc tgtactccac cccacccatc tagaacccag ctttcttgta 4081 caaagtggtg atatccaatt aaccctcact aaagggagaa tgttgaataa gaagtaattc 4141 cctatagtga gtcgtattac cacccaactt ttctatacaa agtggttgat atccagcaca 4201 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc 4261 tcctcggtct cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg 4321 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 4381 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 4441 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 4501 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 4561 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 4621 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 4681 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 4741 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 4801 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 4861 gatgataata tggccacaca tatgaccgag tacaagccca cggtgcgcct cgccacccgc 4921 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 4981 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 5041 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 5101 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 5161 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 5221 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 5281 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 5341 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 5401 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 5461 acccgcaagc ccggtgccta aatcgataga tcctaatcaa cctctggatt acaaaatttg 5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 6061 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct 6121 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 6181 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 6241 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 6301 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 6361 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 6421 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa 6481 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa 6541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 6601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 6661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 6721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 6781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 6841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 6901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga 6961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 7021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 7081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 7141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 7201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 7261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 7321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 7381 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 7441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 7501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 7561 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 7621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 7681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 7741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 7801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 7861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 7921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 7981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 8041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 8101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 8161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 8221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 8281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 8341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 8401 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 8461 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 8521 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt 8581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 8641 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga 8701 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 8761 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 8821 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc 8881 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa 8941 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac 9001 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 9061 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc 9121 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 9181 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 9241 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 9301 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 9361 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 9421 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 9481 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 9541 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 9601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 9661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 9721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag 9781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc 9841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt 9901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa 9961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag 10021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg 10081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag 10141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag 10201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct 10261 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata 10321 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt 10381 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc 10441 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc 10501 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt 10561 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata 10621 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac 10681 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac 10741 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat 10801 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg 10861 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat 10921 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa 10981 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg 11041 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat 11101 tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac 11161 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 11221 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt 11281 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc 11341 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 11401 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 11461 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 11521 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc 11581 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc 11641 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 11701 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg 11761 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 11821 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 11881 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 11941 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 12001 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 12061 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 12121 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 12181 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 12241 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 12301 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 12361 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 12421 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 12481 tttttcttcc atttcaggtg tcgtga //