LOCUS Exported 6611 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For expression of the genetically encoded dopamine sensor dLight1.3b. ACCESSION . VERSION . KEYWORDS pAAV-CAG-dLight1.3b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6611) AUTHORS Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L TITLE Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors. JOURNAL Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 10.1126/science.aat4422. Epub 2018 May 31. PUBMED 29853555 REFERENCE 2 (bases 1 to 6611) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6611 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 162..291 /label=AAV2 ITR primer_bind 1158..1180 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1242..1261 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 2272..2293 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(2399..2420) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2660..2679) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" misc_feature 3365..3953 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3418..3438) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3836..3847) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3996..4117 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4007..4026) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4061..4080 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(4325..4342) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4496..5084) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4576..4595) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5255..6115) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5878..5897 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6116..6220) /gene="bla" /label=AmpR promoter rep_origin complement(6246..90) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6383..6404) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6595..3 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 61 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt 121 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct cgctcgctca 181 ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga 241 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa 301 tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagagt accattgacg 361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 421 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 481 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 541 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 601 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca 661 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg 721 gggcgcgcgc crggsggggs gggggggggs gggggggggg gsggggsgrg gsggrgrggk 781 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg 841 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt 901 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg 961 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg 1021 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag 1081 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg 1141 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc 1201 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt 1261 gctgtctcat cattttggca aagaattgga tccaccggtg cgcgccatga agacgatcat 1321 cgccctgagc tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccat 1381 gaggactctg aacacctctg ccatggacgg gactgggctg gtggtggaga gggacttctc 1441 tgttcgtatc ctcactgcct gtttcctgtc gctgctcatc ctgtccacgc tcctggggaa 1501 cacgctggtc tgtgctgccg ttatcaggtt ccgacacctg cggtccaagg tgaccaactt 1561 ctttgtcatc tccttggctg tgtcagatct cttggtggcc gtcctggtca tgccctggaa 1621 ggcagtggct gagattgctg gcttctggcc ctttgggtcc ttctgtaaca tctgggtggc 1681 ctttgacatc atgtgctcca ctgcatccat cctcaacctc tgtgtgatca gcgtggacag 1741 gtattgggct atctccagcc ctgcccggta tgagagaaag atgaccccca aggcagcctt 1801 catcctgatc agtgtggcat ggaccttgtc tgtactcatc tccttcatcc cagtgcagct 1861 cagctggcac aaggcaaaac ccacaagccc ctctgatgga aatgccactt ccctggctga 1921 gaccatagac aactgtgact ccagcctcag caggacatat gccatctcat cctctgtaat 1981 cagcttttac atccctgtgg ccatcatgat tgtcacctac accaggatct acaggattgc 2041 tcagaaacag ctgagctcac tcattaacgt ctatatcaag gccgacaagc agaagaacgg 2101 catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta 2161 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2221 cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct 2281 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg 2341 taccggaggg agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg 2461 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 2521 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga 2641 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 2701 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 2761 catcctgggg cacaagctgg agtacaacaa tcatgaccaa ctgaaaagag aaactaaagt 2821 cctgaagact ctgtcggtga tcatgggtgt gtttgtgtgc tgttggctac ctttcttcat 2881 cttgaactgc attttgccct tctgtgggtc tggggagacg cagcccttct gcattgattc 2941 caacaccttt gacgtgtttg tgtggtttgg gtgggctaat tcatccttga accccatcat 3001 ttatgccttt aatgctgatt ttcggaaggc attttcaacc ctcttaggat gctacagact 3061 ttgccctgcg acgaataatg ccatagagac ggtgagtatc aataacaatg gggccgcgat 3121 gttttccagc catcatgagc cacgaggctc catctccaag gagtgcaatc tggtttacct 3181 gatcccacat gctgtgggct cctctgagga cctgaaaaag gaggaggcag ctggcatcgc 3241 cagacccttg gagaagctgt ccccagccct atcggtcata ttggactatg acactgacgt 3301 ctctctggag aagatccaac ccatcacaca aaacggtcag cacccaacct gaaagcttat 3361 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 3421 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 3481 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 3541 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 3601 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 3661 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 3721 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 3781 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 3841 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 3901 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata 3961 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca 4021 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 4081 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 4141 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa 4201 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg 4261 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg 4321 ctcgctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 4381 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 4441 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 4501 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 4561 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 4621 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 4681 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 4741 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 4801 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 4861 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4921 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4981 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 5041 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 5101 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 5161 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 5221 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 5281 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 5341 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 5401 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 5461 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 5521 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 5581 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 5641 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 5701 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 5761 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 5821 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 5881 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 5941 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 6001 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 6061 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 6121 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 6181 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 6241 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 6301 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 6361 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 6421 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 6481 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 6541 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 6601 gaaagcgaaa g //