LOCUS Exported 9807 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo. ACCESSION . VERSION . KEYWORDS pLenti CMV Neo DEST (705-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9807) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 9807) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9807 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 26..130 /gene="bla" /label=AmpR promoter CDS 131..991 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(349..368) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1162..1750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1651..1670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1904..1921 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2038..2059 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2074..2104 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2112..2128 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2117..2139 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2136..2152 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2136..2152 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2171..2191 /label=T3 /note="T3 promoter, forward primer" promoter 2173..2191 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2219..2445 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2446..2626 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2673..2798 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3291..3524 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3709..3753 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4020..4136 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4159..4462 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4463..4666 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4617..4637 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4663..4687 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 4771..4895 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4920..4950 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 5004..5663 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(5070..5089) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 6005..6310 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6189..6208 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(6351..6475) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 6506..7094 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6559..6579) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6977..6988) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 7137..7636 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7172..7195) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7569..7588 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7650..8453 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7713..7732) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8323..8342 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 8544..8777 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8849..8970 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8886..8905) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8940..8959 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9010..9145 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9072..9091 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9165..9184) /label=T7 /note="T7 promoter, forward primer" promoter complement(9166..9184) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9194..9211) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9194..9210) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9203..9225) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9352..9807 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9439..9458) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9649..9670 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta 2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat 3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg 4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta 4741 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc tgaacgagaa 4801 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca 4861 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct 4921 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat 4981 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata 5041 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct 5101 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc 5161 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat 5221 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca 5281 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt 5341 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct 5401 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt 5461 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca 5521 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc 5581 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg 5641 atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt 5701 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa 5761 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag 5821 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg 5881 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg 5941 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg 6001 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga 6061 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc 6121 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga 6181 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga 6241 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg 6301 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact 6361 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata 6421 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata 6481 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt 6541 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 6601 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 6661 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 6721 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 6781 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 6841 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 6901 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 6961 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 7021 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 7081 ggccgcctcc ccgcctggaa ttctgcagat atccggacta gtgatctaat tctaccgggt 7141 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 7201 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 7261 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 7321 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 7381 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 7441 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 7501 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 7561 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 7621 atctccgggc ctttcgacct gcagccaata tgggatcggc cattgaacaa gatggattgc 7681 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 7741 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 7801 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 7861 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 7921 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 7981 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 8041 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 8101 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 8161 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 8221 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 8281 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 8341 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 8401 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc 8461 cgtcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 8521 ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct 8581 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 8641 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 8701 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 8761 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 8821 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 8881 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 8941 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 9001 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 9061 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 9121 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 9181 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 9241 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 9301 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 9361 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 9421 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 9481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 9541 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 9601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 9661 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 9721 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 9781 ttaacaaaat attaacgctt acaattt //