LOCUS Exported 5364 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning vector containing A-B-C casette. ACCESSION . VERSION . KEYWORDS pOVC1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5364) AUTHORS Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H TITLE Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions. JOURNAL J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PUBMED 31150735 REFERENCE 2 (bases 1 to 5364) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5364 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(958..975) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 964..1188 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1234..1662 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1321..1340) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1531..1552 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(1671..1691) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1676..2005 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1856..1991 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1918..1937 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2072..2866 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2126..2145) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2736..2755 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3040..3161 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3077..3096) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3131..3150 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3210..3226) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3210..3226) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3223..3245) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3234..3250 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3258..3288) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3303..3324 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3441..3458) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3612..4197) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3692..3711) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4368..5228) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4991..5010 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5229..5333) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctgaccatg 901 gccaccggtg aattctcagg ctctggatcc cccgggtaag tttaaaccgc tgatcagcct 961 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1021 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1081 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1141 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 1201 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 1261 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1321 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1381 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1441 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 1501 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1561 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 1621 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 1681 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 1741 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 1801 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 1861 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 1921 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 1981 ttttggaggc ctaggctttt gcaaaaagct ctcgggagct tgtatatcca ttttcggatc 2041 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2101 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 2161 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 2221 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 2281 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 2341 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 2401 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 2461 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 2521 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 2581 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 2641 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 2701 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 2761 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 2821 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 2881 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 2941 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 3001 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat 3061 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 3121 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg 3181 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 3241 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 3301 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 3361 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 3421 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 3481 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 3541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 3601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 3661 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 3721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 3781 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 3841 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 3901 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3961 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 4021 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 4081 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 4141 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4501 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 5041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 5101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5341 ccccgaaaag tgccacctga cgtc //