LOCUS Exported 5219 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral vector for cloning and expressing your gene of interest. Hygromycin selection.. ACCESSION . VERSION . KEYWORDS pBABE-hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5219) AUTHORS Morgenstern JP, Land H TITLE Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. JOURNAL Nucleic Acids Res. 1990 Jun 25;18(12):3587-96. PUBMED 2194165 REFERENCE 2 (bases 1 to 5219) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5219 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..470 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 535..734 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 935..1351 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1281..1303 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1319..1335 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(1409..1429) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1414..1743 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1594..1729 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1656..1675 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1817..2842 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" primer_bind 3359..3378 /label=pBR322ori-F /note="pBR322 origin, forward primer" LTR 3640..4109 /note="long terminal repeat from Moloney murine leukemia virus" CDS complement(4224..5084) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4847..4866 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5085..5189) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg 361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg 541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct 601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga 661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg 721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct 781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg 841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg 901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag 961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt 1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg 1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc 1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc 1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc 1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct 1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg 1381 aattcgccag cacagtggtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc 1441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 1501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 1561 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 1621 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 1681 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 1741 aaaaagcttg ccagctgggg cgccctctgg taaggttggg aagccctgca gatctgatcc 1801 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 1861 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 1921 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 1981 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 2041 ggaagtgctt gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc 2101 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 2161 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 2221 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 2281 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 2341 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 2401 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 2461 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 2521 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 2581 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 2641 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 2701 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 2761 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 2821 tcgtccgagg gcaaaggaat agagtagatg ccgaccggga tctatcgatg cggcgggtgt 2881 ggtggttacg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 2941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 3001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 3061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 3121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 3181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 3241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 3301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 3361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 3421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcatcgataa 3481 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt 3541 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa 3601 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga 3661 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat 3721 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag 3781 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag 3841 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt 3901 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca 3961 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc 4021 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg 4081 attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt 4141 tttttcttaa gtatttacat taaatggcca tagtgcggcc gcaaatcaat ctaaagtata 4201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 4261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 4381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 4441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 4501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 4561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 4861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 5041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 5101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 5161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcagc //