LOCUS Exported 5073 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCAX APP C99 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5073) AUTHORS Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ TITLE A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference. JOURNAL J Neurosci. 2007 Dec 26. 27(52):14459-69. PUBMED 18160654 REFERENCE 2 (bases 1 to 5073) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5073 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 810..832 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 894..913 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind complement(1307..1326) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 1372..1427 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(1426..1445) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(1788..1804) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1788..1804) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1801..1823) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1812..1828 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1836..1866) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1881..1902 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 1961..2156 /label=SV40 promoter /note="SV40 early promoter" rep_origin 2007..2142 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2069..2088 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 2162..2296 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2199..2218) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2253..2272 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(2364..2381) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2535..3123) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2615..2634) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3294..4154) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3917..3936 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4155..4259) /gene="bla" /label=AmpR promoter enhancer 4290..4669 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" intron 4943..886 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" ORIGIN 1 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg 61 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg 121 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg 181 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc 241 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg 301 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg 361 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct 421 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg 481 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg 541 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc 601 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg 661 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc 721 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg 781 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc 841 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt 901 gctggttatt gtgctgtctc atcattttgg caaagaattg gccgctcgag ccatgctgcc 961 cggtttggca ctgctcctgc tggccgcctg gacggctcgg gcggatgcag aattccgaca 1021 tgactcagga tatgaagttc atcatcaaaa attggtgttc tttgcagaag atgtgggttc 1081 aaacaaaggt gcaatcattg gactcatggt gggcggtgtt gtcatagcga cagtgatcgt 1141 catcaccttg gtgatgctga agaagaaaca gtacacatcc attcatcatg gtgtggtgga 1201 ggttgacgcc gctgtcaccc cagaggagcg ccacctgtcc aagatgcagc agaacggcta 1261 cgaaaatcca acctacaagt tctttgagca gatgcagaat tagagatctt tttccctctg 1321 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 1381 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 1441 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 1501 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga 1561 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt 1621 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg 1681 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc 1741 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt 1801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 1861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 1921 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa 1981 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2041 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 2101 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 2161 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 2221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 2281 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg 2341 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 2401 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 2461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 2521 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 2581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 2641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 2701 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 2761 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 2821 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 2881 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 2941 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 3001 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3061 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3121 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3181 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 3241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 3301 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 3361 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 3421 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 3481 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 3541 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 3601 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 3661 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 3721 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 3781 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 3841 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 3901 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 3961 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 4021 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 4081 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 4141 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 4201 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 4261 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta 4321 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 4381 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 4441 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 4501 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 4561 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 4621 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag 4681 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt 4741 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggccaggcgg 4801 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 4861 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 4921 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 4981 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 5041 cgggcgggac ggcccttctc ctccgggctg taa //