LOCUS Exported 4681 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: N1 Cloning Vector, Excitation: 440, Emission: 620. ACCESSION . VERSION . KEYWORDS mKeima-Red-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4681) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 4681) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4681 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 97..400 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 401..604 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 555..575 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 627..707 /label=MCS /note="multiple cloning site" regulatory 709..718 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 715..1383 /codon_start=1 /product="monomeric Keima-Red fluorescent protein, also known as mKeima" /label=mKeima-Red /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG " polyA_signal 1505..1626 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1542..1561) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1596..1615 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1633..2088) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1770..1791) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1982..2001 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2115..2219 /gene="bla" /label=AmpR promoter promoter 2221..2578 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2244..2264) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2429..2564 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2491..2510 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2613..3407 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2667..2686) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3277..3296 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3595..3614) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3639..3686 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4015..4603 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4504..4523 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac 61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 601 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag 661 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg 721 agtgtgatcg ctaaacaaat gacctacaag gtttatatgt caggcacggt caatggacac 781 tactttgagg tcgaaggcga tggaaaagga aagccttacg agggagagca gacagtaaag 841 ctcactgtca ccaagggtgg acctctgcca tttgcttggg atattttatc accacagctt 901 cagtacggaa gcataccatt caccaagtac cctgaagaca tccctgatta tttcaagcag 961 tcattccctg agggatatac atgggagagg agcatgaact ttgaagatgg tgcagtgtgt 1021 actgtcagca atgattccag catccaaggc aactgtttca tctacaatgt caaaatctct 1081 ggtgagaact ttcctcccaa tggacctgtt atgcagaaga agacacaggg ctgggaaccc 1141 agcactgagc gtctctttgc acgagatgga atgctgatag gaaacgatta tatggctctg 1201 aagttggaag gaggtggtca ctatttgtgt gaatttaaat ctacttacaa ggcaaagaag 1261 cctgtgagga tgccagggcg ccacgagatt gaccgcaaac tggatgtaac cagtcacaac 1321 agggattaca catctgttga gcagtgtgaa atagccattg cacgccactc tttgctcggt 1381 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc 1441 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 1501 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 1561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 1621 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 1681 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 1741 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 1801 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 1861 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 1921 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 1981 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 2041 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt 2101 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 2161 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc 2221 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 2281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 2341 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 2401 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 2461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 2521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga 2581 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 2641 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 2701 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 2761 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 2821 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 2881 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 2941 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 3001 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 3061 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 3121 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 3181 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 3241 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 3301 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 3361 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 3421 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 3481 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 3541 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac 3601 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 3661 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 3721 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 3781 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 3841 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta 3901 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3961 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 4021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 4081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 4141 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 4201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 4261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 4501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 4561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 4621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 4681 c //