LOCUS Exported 12604 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of FLT4 F131S. ACCESSION . VERSION . KEYWORDS pHAGE-FLT4-F131S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12604) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12604) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12604 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7541..7565) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 7618..7659 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7680..8247 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7697..8249 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7841..7858) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 8068..8087 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8261..8980 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 8261..8977 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8306..8327) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8567..8586) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8914..8935 /label=EGFP-C /note="EGFP, forward primer" misc_feature 8994..9582 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9047..9067) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9465..9476) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9657..9890 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(10009..10027) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10095..10199 /gene="bla" /label=AmpR promoter CDS 10200..11060 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10418..10437) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11231..11819 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11720..11739 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11973..11990 /label=L4440 /note="L4440 vector, forward primer" primer_bind 12082..12101 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 12230..12251 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12266..12296 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12304..12320 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12309..12331 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12328..12344 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12328..12344 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12394..12413 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cagcggggcg ccgcgctgtg cctgcgactg 3481 tggctctgcc tgggactcct ggacggcctg gtgagtggct actccatgac ccccccgacc 3541 ttgaacatca cggaggagtc acacgtcatc gacaccggtg acagcctgtc catctcctgc 3601 aggggacagc accccctcga gtgggcttgg ccaggagctc aggaggcgcc agccaccgga 3661 gacaaggaca gcgaggacac gggggtggtg cgagactgcg agggcacaga cgccaggccc 3721 tactgcaagg tgttgctgct gcacgaggta catgccaacg acacaggcag ctacgtctgc 3781 tactacaagt acatcaaggc acgcatcgag ggcaccacgg ccgccagctc ctacgtgtcc 3841 gtgagagact ttgagcagcc attcatcaac aagcctgaca cgctcttggt caacaggaag 3901 gacgccatgt gggtgccctg tctggtgtcc atccccggcc tcaatgtcac gctgcgctcg 3961 caaagctcgg tgctgtggcc agacgggcag gaggtggtgt gggatgaccg gcggggcatg 4021 ctcgtgtcca cgccactgct gcacgatgcc ctgtacctgc agtgcgagac cacctgggga 4081 gaccaggact tcctttccaa ccccttcctg gtgcacatca caggcaacga gctctatgac 4141 atccagctgt tgcccaggaa gtcgctggag ctgctggtag gggagaagct ggtcctgaac 4201 tgcaccgtgt gggctgagtt taactcaggt gtcacctttg actgggacta cccagggaag 4261 caggcagagc ggggtaagtg ggtgcccgag cgacgctccc agcagaccca cacagaactc 4321 tccagcatcc tgaccatcca caacgtcagc cagcacgacc tgggctcgta tgtgtgcaag 4381 gccaacaacg gcatccagcg atttcgggag agcaccgagg tcattgtgca tgaaaatccc 4441 ttcatcagcg tcgagtggct caaaggaccc atcctggagg ccacggcagg agacgagctg 4501 gtgaagctgc ccgtgaagct ggcagcgtac cccccgcccg agttccagtg gtacaaggat 4561 ggaaaggcac tgtccgggcg ccacagtcca catgccctgg tgctcaagga ggtgacagag 4621 gccagcacag gcacctacac cctcgccctg tggaactccg ctgctggcct gaggcgcaac 4681 atcagcctgg agctggtggt gaatgtgccc ccccagatac atgagaagga ggcctcctcc 4741 cccagcatct actcgcgtca cagccgccag gccctcacct gcacggccta cggggtgccc 4801 ctgcctctca gcatccagtg gcactggcgg ccctggacac cctgcaagat gtttgcccag 4861 cgtagtctcc ggcggcggca gcagcaagac ctcatgccac agtgccgtga ctggagggcg 4921 gtgaccacgc aggatgccgt gaaccccatc gagagcctgg acacctggac cgagtttgtg 4981 gagggaaaga ataagactgt gagcaagctg gtgatccaga atgccaacgt gtctgccatg 5041 tacaagtgtg tggtctccaa caaggtgggc caggatgagc ggctcatcta cttctatgtg 5101 accaccatcc ccgacggctt caccatcgaa tccaagccat ccgaggagct actagagggc 5161 cagccggtgc tcctgagctg ccaagccgac agctacaagt acgagcatct gcgctggtac 5221 cgcctcaacc tgtccacgct gcacgatgcg cacgggaacc cgcttctgct cgactgcaag 5281 aacgtgcatc tgttcgccac ccctctggcc gccagcctgg aggaggtggc acctggggcg 5341 cgccacgcca cgctcagcct gagtatcccc cgcgtcgcgc ccgagcacga gggccactat 5401 gtgtgcgaag tgcaagaccg gcgcagccat gacaagcact gccacaagaa gtacctgtcg 5461 gtgcaggccc tggaagcccc tcggctcacg cagaacttga ccgacctcct ggtgaacgtg 5521 agcgactcgc tggagatgca gtgcttggtg gccggagcgc acgcgcccag catcgtgtgg 5581 tacaaagacg agaggctgct ggaggaaaag tctggagtcg acttggcgga ctccaaccag 5641 aagctgagca tccagcgcgt gcgcgaggag gatgcgggac gctatctgtg cagcgtgtgc 5701 aacgccaagg gctgcgtcaa ctcctccgcc agcgtggccg tggaaggctc cgaggataag 5761 ggcagcatgg agatcgtgat ccttgtcggt accggcgtca tcgctgtctt cttctgggtc 5821 ctcctcctcc tcatcttctg taacatgagg aggccggccc acgcagacat caagacgggc 5881 tacctgtcca tcatcatgga ccccggggag gtgcctctgg aggagcaatg cgaatacctg 5941 tcctacgatg ccagccagtg ggaattcccc cgagagcggc tgcacctggg gagagtgctc 6001 ggctacggcg ccttcgggaa ggtggtggaa gcctccgctt tcggcatcca caagggcagc 6061 agctgtgaca ccgtggccgt gaaaatgctg aaagagggcg ccacggccag cgagcaccgc 6121 gcgctgatgt cggagctcaa gatcctcatt cacatcggca accacctcaa cgtggtcaac 6181 ctcctcgggg cgtgcaccaa gccgcagggc cccctcatgg tgatcgtgga gttctgcaag 6241 tacggcaacc tctccaactt cctgcgcgcc aagcgggacg ccttcagccc ctgcgcggag 6301 aagtctcccg agcagcgcgg acgcttccgc gccatggtgg agctcgccag gctggatcgg 6361 aggcggccgg ggagcagcga cagggtcctc ttcgcgcggt tctcgaagac cgagggcgga 6421 gcgaggcggg cttctccaga ccaagaagct gaggacctgt ggctgagccc gctgaccatg 6481 gaagatcttg tctgctacag cttccaggtg gccagaggga tggagttcct ggcttcccga 6541 aagtgcatcc acagagacct ggctgctcgg aacattctgc tgtcggaaag cgacgtggtg 6601 aagatctgtg actttggcct tgcccgggac atctacaaag accccgacta cgtccgcaag 6661 ggcagtgccc ggctgcccct gaagtggatg gcccctgaaa gcatcttcga caaggtgtac 6721 accacgcaga gtgacgtgtg gtcctttggg gtgcttctct gggagatctt ctctctgggg 6781 gcctccccgt accctggggt gcagatcaat gaggagttct gccagcggct gagagacggc 6841 acaaggatga gggccccgga gctggccact cccgccatac gccgcatcat gctgaactgc 6901 tggtccggag accccaaggc gagacctgca ttctcggagc tggtggagat cctgggggac 6961 ctgctccagg gcaggggcct gcaagaggaa gaggaggtct gcatggcccc gcgcagctct 7021 cagagctcag aagagggcag cttctcgcag gtgtccacca tggccctaca catcgcccag 7081 gctgacgctg aggacagccc gccaagcctg cagcgccaca gcctggccgc caggtattac 7141 aactgggtgt cctttcccgg gtgcctggcc agaggggctg agacccgtgg ttcctccagg 7201 atgaagacat ttgaggaatt ccccatgacc ccaacgacct acaaaggctc tgtggacaac 7261 cagacagaca gtgggatggt gctggcctcg gaggagtttg agcagataga gagcaggcat 7321 agacaagaaa gcggcttcag ctgtaaagga cctggccaga atgtggctgt gaccagggca 7381 caccctgact cccaagggag gcggcggcgg cctgagcggg gggcccgagg aggccaggtg 7441 ttttacaaca gcgagtatgg ggagctgtcg gagccaagcg aggaggacca ctgctccccg 7501 tctgcccgcg tgactttctt cacagacaac agctactaga acccagcttt cttgtacaaa 7561 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt 7621 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat 7681 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 7741 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 7801 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 7861 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 7921 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 7981 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 8041 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg 8101 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 8161 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg 8221 gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca agggcgagga 8281 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 8341 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 8401 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 8461 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 8521 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 8581 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 8641 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 8701 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 8761 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 8821 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 8881 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 8941 cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat cctaatcaac 9001 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 9061 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 9121 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 9181 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 9241 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 9301 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 9361 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 9421 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 9481 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 9541 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc 9601 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 9661 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 9721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 9781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 9841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 9901 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 9961 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac 10021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 10081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 10141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 10201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 10261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 10321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 10381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 10441 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 10501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 10561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 10621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 10681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 10741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 10801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 10861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 10921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 10981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 11041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 11101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 11161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 11221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 11281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 11341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 11401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 11461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 11521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 11581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 11641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 11701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 11761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 11821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 11881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 11941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 12001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc 12061 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 12121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt 12181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 12241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 12301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca 12361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 12421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca 12481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt 12541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag 12601 tagt //