LOCUS Exported 10329 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro. ACCESSION . VERSION . KEYWORDS pLenti CMV Hygro DEST (w117-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10329) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 10329) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10329 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2220..2446 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2447..2627 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2674..2799 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3292..3525 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3710..3754 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4021..4137 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4160..4463 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4464..4667 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4618..4638 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4664..4688 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 4772..4896 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4921..4951 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 5005..5664 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(5071..5090) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 6006..6311 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6190..6209 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(6352..6476) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 6507..7095 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6560..6580) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6978..6989) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 7151..7650 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7186..7209) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7583..7602 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7668..8693 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" LTR 9067..9300 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9372..9493 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9409..9428) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9463..9482 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9533..9668 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9595..9614 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9688..9707) /label=T7 /note="T7 promoter, forward primer" promoter complement(9689..9707) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9717..9734) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9717..9733) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9726..9748) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9875..1 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9962..9981) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10172..10193 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt 2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac 2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct 2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact 2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt 2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa 2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa 2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca 3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata 3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag 3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa 3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta 3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca 3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg 3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt 3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca 3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg 3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac 4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 4141 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc 4681 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct 4741 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ctgaacgaga 4801 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac 4861 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc 4921 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga 4981 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat 5041 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc 5101 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag 5161 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa 5221 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 5281 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 5341 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 5401 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 5461 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 5521 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 5581 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 5641 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag 5701 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga 5761 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca 5821 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat 5881 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat 5941 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg 6001 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg 6061 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg 6121 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg 6181 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag 6241 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct 6301 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac 6361 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 6421 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat 6481 atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat 6541 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 6601 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 6661 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 6721 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 6781 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 6841 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 6901 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 6961 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 7021 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 7081 gggccgcctc cccgcctgga attctgcaga tatccggact agtgatctct cgaggttaac 7141 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca 7201 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc 7261 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct 7321 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg 7381 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg 7441 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg 7501 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc 7561 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg 7621 ttctcctctt cctcatctcc gggcctttcg acctgcatcc cgccaccatg aaaaagcctg 7681 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc 7741 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg 7801 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc 7861 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg 7921 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg 7981 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg 8041 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca 8101 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg 8161 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg 8221 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc 8281 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt 8341 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc 8401 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt 8461 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg 8521 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg 8581 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac 8641 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat 8701 gccgaccgaa caagagctga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc 8761 aaggcaaatg cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct 8821 aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc ttctggattg 8881 tgttggtccc cagggcacga ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc 8941 agattggaga tcgccgcccg tgcctgccga ttgggtgcag atccgtcgag ggcccggtac 9001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 9061 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg 9121 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 9181 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 9241 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 9301 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 9361 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 9421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 9481 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 9541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 9601 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 9661 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 9721 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 9781 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 9841 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 9901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 9961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 10021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 10081 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 10141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 10201 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 10261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 10321 cttacaatt //