LOCUS Exported 9254 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon). ACCESSION . VERSION . KEYWORDS pORANGE GFP-Camk2a KI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9254) AUTHORS Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD TITLE ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. JOURNAL PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. PUBMED 32275651 REFERENCE 2 (bases 1 to 9254) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9254 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 39..279 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 39..59 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 210..229 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 308..383 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS 505..1221 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 505..1221 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(550..571) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(811..830) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1158..1179 /label=EGFP-C /note="EGFP, forward primer" enhancer 1296..1581 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 1583..1860 /label=chicken beta-actin promoter intron 1861..2088 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" CDS 2109..2135 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 2139..2159 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2169..6269 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6273..6293 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(6333..6350) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6339..6546 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6555..6695 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6555..6684 /label=AAV2 ITR rep_origin 6770..7225 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6857..6876) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7067..7088 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7242..7261) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7361..7383 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7421..7439) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7507..7611 /gene="bla" /label=AmpR promoter CDS 7612..8472 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7830..7849) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8643..9231 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9132..9151 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 acggttcctg gccttttgct ggccttttgc tcacatgtga gggcctattt cccatgattc 61 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg 121 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt 181 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt 241 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccctg cctgcccagt 301 gccaggagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg 361 aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata 421 aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaagcttcc 481 ctcctggcac tgggcaggca gaggatggtg agcaagggcg aggagctgtt caccggggtg 541 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 601 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 661 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 721 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 781 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 841 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 901 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 961 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 1021 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 1081 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 1141 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 1201 ggcatggacg agctgtacaa gggatcagcc ctcctggcac tgggcaggca gctcgaggat 1261 cctaggacgc gtcctaggac gcgtccagag gtacccgtta cataacttac ggtaaatggc 1321 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg 1381 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 1441 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 1501 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 1561 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc 1621 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg 1681 atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg 1741 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 1801 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 1861 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg 1921 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct 1981 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta 2041 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg 2101 ccaccatgta cccatacgat gttccagatt acgcttcgcc gaagaaaaag cgcaaggtcg 2161 aagcgtccga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg 2221 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg 2281 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag 2341 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga 2401 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc 2461 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct 2521 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga 2581 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg 2641 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca 2701 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa 2761 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga 2821 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg 2881 gcaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg 2941 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc 3001 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg 3061 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg 3121 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg 3181 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct 3241 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca 3301 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc 3361 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc 3421 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa 3481 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa 3541 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg 3601 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg 3661 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca 3721 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct 3781 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag 3841 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg 3901 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga 3961 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata 4021 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct 4081 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct 4141 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa 4201 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg 4261 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata 4321 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc 4381 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca 4441 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg 4501 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac 4561 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg 4621 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg 4681 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca 4741 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga 4801 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca 4861 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga 4921 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga 4981 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga 5041 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca 5101 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca 5161 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc 5221 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct 5281 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga 5341 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg 5401 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg 5461 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc 5521 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg 5581 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga 5641 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca 5701 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc 5761 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg 5821 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg 5881 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg 5941 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga 6001 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg 6061 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg 6121 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca 6181 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac 6241 ggatcgacct gtctcagctg ggaggcgaca gccccaagaa gaagagaaag gtggaggcca 6301 gctaagaatt ctaactagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc 6361 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 6421 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 6481 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc 6541 tggggagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 6601 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 6661 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct 6721 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt 6781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 6841 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 6901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 6961 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga 7021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 7081 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg 7141 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 7201 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc 7261 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 7321 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 7381 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 7441 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 7501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 7561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 7621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 7681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 7741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 7801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 7861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 7921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 7981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 8041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 8101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 8161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 8221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 8281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc 8341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 8401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 8461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 8521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 8581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 8641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 8701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 8761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 8821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 8881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 8941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 9001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 9061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 9121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 9181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 9241 aacgcggcct tttt //