LOCUS Exported 2983 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For course in ""Synthetic Biology: A Lab Manual"". ACCESSION . VERSION . KEYWORDS promoter J23110 (upstream of RBS, mRFP1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2983) AUTHORS Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC TITLE Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. JOURNAL J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. PUBMED 29760772 REFERENCE 2 (bases 1 to 2983) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2983 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..44 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 47..68 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" RBS 112..123 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 130..807 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 852..923 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 939..966 /label=T7Te terminator /note="phage T7 early transcription terminator" primer_bind complement(999..1016) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1170..1758) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1250..1269) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1929..2789) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2552..2571 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2790..2894) /gene="bla" /label=AmpR promoter primer_bind 2962..2980 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactagt gaaagaggag 121 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt 181 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg 241 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct 301 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct 361 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg 421 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag 481 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag 541 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg 601 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt 661 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc 721 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct 781 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta 841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 961 tctgcgttta tatactagaa gcggccgctg caggcttcct cgctcactga ctcgctgcgc 1021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 1081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 1141 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat 1201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 1261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 1321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 1381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 1441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 1501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 1561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 1621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 1681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 1741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 1801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 1861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 1921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 1981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 2041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 2101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 2161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 2221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 2281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 2341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 2401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 2461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 2521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 2581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 2641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 2701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 2761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 2821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 2881 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 2941 atcatgacat taacctataa aaataggcgt atcacgaggc aga //