LOCUS Exported 2787 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For course in ""Synthetic Biology: A Lab Manual"". ACCESSION . VERSION . KEYWORDS amilGFP chromoprotein promoter-less SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2787) AUTHORS Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC TITLE Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. JOURNAL J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. PUBMED 29760772 REFERENCE 2 (bases 1 to 2787) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2787 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..44 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 47..68 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" RBS 69..80 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" misc_feature 786..806 /label=BioBrick suffix /note="universal suffix for all parts" terminator 807..864 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(888..905) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1059..1647) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1139..1158) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1830..1924 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1945..2604) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2519..2538 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2766..2784 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 61 ttctagagaa agaggagaaa tactagatgt cttattcaaa gcatggcatc gtacaagaaa 121 tgaagacgaa ataccatatg gaaggcagtg tcaatggcca tgaatttacg atcgaaggtg 181 taggaactgg gtacccttac gaagggaaac agatgtccga attagtgatc atcaagcctg 241 cgggaaaacc ccttccattc tcctttgaca tactgtcatc agtctttcaa tatggaaacc 301 gttgcttcac aaagtacccg gcagacatgc ctgactattt caagcaagca ttcccagatg 361 gaatgtcata tgaaaggtca tttctatttg aggatggagc agttgctaca gccagctgga 421 acattcgtct cgaaggaaat tgcttcatcc acaaatccat ctttcatggc gtaaactttc 481 ccgctgatgg acccgtaatg aaaaagaaga caattgactg ggataagtcc ttcgaaaaaa 541 tgactgtgtc taaagaggtg ctaagaggtg acgtgactat gtttcttatg ctcgaaggag 601 gtggttctca cagatgccaa tttcactcca cttacaaaac agagaagccg gtcacactgc 661 ccccgaatca tgtcgtagaa catcaaattg tgaggaccga ccttggccaa agtgcaaaag 721 gctttacagt caagctggaa gcacatgccg cggctcatgt taaccctttg aaggttaaat 781 aataatacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc 841 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac 901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct 1081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1801 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa 1861 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg 1921 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg 1981 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat 2041 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg 2101 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg 2161 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc 2221 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta 2281 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 2341 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc 2401 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt 2461 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc 2521 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt 2581 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc 2641 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 2701 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 2761 ataaaaatag gcgtatcacg aggcaga //