LOCUS       Exported                2821 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    meffBlue chromoprotein
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..44
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    820..840
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      841..898
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(922..939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1093..1681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1173..1192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1864..1958
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1979..2638)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2553..2572
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2800..2818
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga ggctctttaa
      121 caatttatca tgactaatag gaggtaacag atgtccgtta tcgcaaccca gatgacgtac
      181 aaagtttata tgtcgggcac cgtgaatggt cattattttg aagtcgaagg tgatggcaaa
      241 ggtcgtccgt atgaaggcga acagaccgtc aaactgaccg tgacgaaagg cggtccgctg
      301 ccgtttgcat gggatattct gagtccgcag tgccaatacg gttccattcc gttcaccaaa
      361 tatccggaag atatcccgga ctacgtcaaa cagagctttc cggaaggttt cacgtgggaa
      421 cgcattatga actttgaaga tggcgctgtg tgcaccgttt caaacgacag ctctatccaa
      481 ggcaactgct tcacgtatca tgtgaaattt tcgggtctga acttcccgcc gaatggcccg
      541 gttatgcaga agaaaaccca aggttgggaa ccgcacagtg aacgtctgtt tgcgcgcggc
      601 ggtatgctga tcggcaacaa tttcatggcc ctgaaactgg aaggcggtgg ccattatctg
      661 tgtgaattta aaaccacgta caaagcgaaa aaaccagtga aaatgccggg ttatcattac
      721 gttgatcgta aactggacgt cacgaaccac aataaagact atacctcagt tgaacagtgt
      781 gaaatcagca tcgcacgcaa gccggtggtc gcctaataat actagtagcg gccgctgcag
      841 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
      901 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1081 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     1861 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1921 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1981 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2041 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2101 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2161 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2221 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2281 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2341 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     2401 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     2461 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2521 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2581 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2641 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2701 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
     2761 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     2821 a
//
