LOCUS Exported 2757 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For course in ""Synthetic Biology: A Lab Manual"". ACCESSION . VERSION . KEYWORDS amilCP chromoprotein promoter-less SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2757) AUTHORS Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC TITLE Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. JOURNAL J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. PUBMED 29760772 REFERENCE 2 (bases 1 to 2757) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2757 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..44 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 47..68 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" RBS 69..80 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" misc_feature 756..776 /label=BioBrick suffix /note="universal suffix for all parts" terminator 777..834 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(858..875) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1029..1617) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1109..1128) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1800..1894 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1915..2574) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2489..2508 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2736..2754 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 61 ttctagagaa agaggagaaa tactagatga gtgtgatcgc taaacaaatg acctacaagg 121 tttatatgtc aggcacggtc aatggacact actttgaggt cgaaggcgat ggaaaaggta 181 agccctacga gggggagcag acggtaaagc tcactgtcac caagggcgga cctctgccat 241 ttgcttggga tattttatca ccacagtgtc agtacggaag cataccattc accaagtacc 301 ctgaagacat ccctgactat gtaaagcagt cattcccgga gggctataca tgggagagga 361 tcatgaactt tgaagatggt gcagtgtgta ctgtcagcaa tgattccagc atccaaggca 421 actgtttcat ctaccatgtc aagttctctg gtttgaactt tcctcccaat ggacctgtca 481 tgcagaagaa gacacagggc tgggaaccca acactgagcg tctctttgca cgagatggaa 541 tgctgctagg aaacaacttt atggctctga agttagaagg aggcggtcac tatttgtgtg 601 aatttaaaac tacttacaag gcaaagaagc ctgtgaagat gccagggtat cactatgttg 661 accgcaaact ggatgtaacc aatcacaaca aggattacac ttcggttgag cagtgtgaaa 721 tttccattgc acgcaaacct gtggtcgcct aataatacta gtagcggccg ctgcagtccg 781 gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt 841 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1021 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 1081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 1141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 1201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 1261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 1321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 1381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 1441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 1501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 1621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 1681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 1741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga 1801 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt 1861 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc 1921 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa 1981 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg 2041 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt 2101 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat 2161 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat 2221 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt 2281 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc 2341 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa 2401 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag 2461 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt 2521 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc 2581 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc 2641 attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct 2701 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcaga //