LOCUS Exported 785 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pKMyc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 785) AUTHORS Joberty G, Petersen C, Gao L, Macara IG TITLE The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. JOURNAL Nat Cell Biol 2000 Aug;2(8):531-9. PUBMED 10934474 REFERENCE 2 (bases 1 to 785) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..785 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 58..87 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" regulatory 134..143 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 147..281 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(184..203) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 238..257 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 350..707 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(373..393) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 558..693 /label=SV40 ori /note="SV40 origin of replication" primer_bind 620..639 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(727..744) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(727..743) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(736..758) /label=M13/pUC Forward /note="In lacZ gene" ORIGIN 1 ggtgtccact cccaggtcca actgcacctc ggttctaagc ttctgcaggt cgacatggaa 61 cagaaactca tctctgaaga ggatctgtct agaggatccc gggctagcgg gcggccgctt 121 gaattcatcg atggccgcca tggcccaact tgtttattgc agcttataat ggttacaaat 181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 241 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgggaattaa ttcggcgcag 301 caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa 361 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 421 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 481 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 661 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctgttaacag 721 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 781 taatc //