LOCUS       Exported                2839 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    meffRed chromoprotein
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..44
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    838..858
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      859..916
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(940..957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1111..1699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1191..1210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1882..1976
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1997..2656)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2571..2590
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2818..2836
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
      121 aaatactaga tggcactgag taaaaatggc ctgaccaaaa acatgacgac caaatatcgt
      181 atggaaggtt gcgtggacgg tcacaagttc gttatcaccg gcgatggtat tggcgacccg
      241 tttgaaggta aacagacgag catcgatctg tgcgtggttg aaggcggtcc gctgccgttc
      301 agtgaagata ttctgtccgc agtttttgac tatggcaacc gtgtcttcac caaatatccg
      361 caggatctgg ttgactactt taagaatagc tgtccggcgg gttacacctg gcaacgctct
      421 tttctgttcg aagacggtgc agtctgcacc gcgtcagccg atattacggt ctcggtggaa
      481 gaaaactgtt tctaccatga aagcaagttt cacggtgtga atttcccggc cgatggcccg
      541 gttatgaaaa agatgaccac gaattgggaa ccgtcttgcg aaaaaattac cccgatcccg
      601 aatgaaggta tcctgaaagg cgacgtcacg atgtttctgc tgctgaagga tggcggtcgt
      661 tatcgctgtc aattcgatac cgtgtacaaa gcaaagagtg acccgaaaac gattatgatg
      721 ccggattggc attttatcca gcacaagctg aaccgtgaag atcgctccga cgctaaacat
      781 caaaagtggc gtctggtgga aaatgcaatc gcttaccgca gcaccctgtc ttaataatac
      841 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
      901 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
      961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     1141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1861 gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     1921 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     1981 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     2041 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     2101 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     2161 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     2221 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     2281 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     2341 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     2401 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     2461 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     2521 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     2581 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     2641 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     2701 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
     2761 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2821 aggcgtatca cgaggcaga
//
