LOCUS Exported 12616 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of IGF1R F1057L. ACCESSION . VERSION . KEYWORDS pHAGE-IGF1R-F1057L SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12616) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12616) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12616 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..1179 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 232..1170 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1127..1147 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1199..1223 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5331..5355) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5408..5449 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5470..6037 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5487..6039 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5631..5648) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5858..5877 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6051..6770 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6051..6767 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6096..6117) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6357..6376) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6704..6725 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6784..7372 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6837..6857) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7255..7266) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7447..7680 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7799..7817) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7885..7989 /gene="bla" /label=AmpR promoter CDS 7990..8850 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8208..8227) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9021..9609 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9510..9529 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9763..9780 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9872..9891 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10020..10041 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10056..10086 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10094..10110 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10099..10121 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10118..10134 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10118..10134 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10184..10203 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10395..11028 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 11075..11200 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11697..11930 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12115..12159 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 12452..12569 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac 1201 aagtttgtac aaaaaagcag gcaccatgaa gtctggctcc ggaggagggt ccccgacctc 1261 gctgtggggg ctcctgtttc tctccgccgc gctctcgctc tggccgacga gtggagaaat 1321 ctgcgggcca ggcatcgaca tccgcaacga ctatcagcag ctgaagcgcc tggagaactg 1381 cacggtgatc gagggctacc tccacatcct gctcatctcc aaggccgagg actaccgcag 1441 ctaccgcttc cccaagctca cggtcattac cgagtacttg ctgctgttcc gagtggctgg 1501 cctcgagagc ctcggagacc tcttccccaa cctcacggtc atccgcggct ggaaactctt 1561 ctacaactac gccctggtca tcttcgagat gaccaatctc aaggatattg ggctttacaa 1621 cctgaggaac attactcggg gggccatcag gattgagaaa aatgctgacc tctgttacct 1681 ctccactgtg gactggtccc tgatcctgga tgcggtgtcc aataactaca ttgtggggaa 1741 taagccccca aaggaatgtg gggacctgtg tccagggacc atggaggaga agccgatgtg 1801 tgagaagacc accatcaaca atgagtacaa ctaccgctgc tggaccacaa accgctgcca 1861 gaaaatgtgc ccaagcacgt gtgggaagcg ggcgtgcacc gagaacaatg agtgctgcca 1921 ccccgagtgc ctgggcagct gcagcgcgcc tgacaacgac acggcctgtg tagcttgccg 1981 ccactactac tatgccggtg tctgtgtgcc tgcctgcccg cccaacacct acaggtttga 2041 gggctggcgc tgtgtggacc gtgacttctg cgccaacatc ctcagcgccg agagcagcga 2101 ctccgagggg tttgtgatcc acgacggcga gtgcatgcag gagtgcccct cgggcttcat 2161 ccgcaacggc agccagagca tgtactgcat cccttgtgaa ggtccttgcc cgaaggtctg 2221 tgaggaagaa aagaaaacaa agaccattga ttctgttact tctgctcaga tgctccaagg 2281 atgcaccatc ttcaagggca atttgctcat taacatccga cgggggaata acattgcttc 2341 agagctggag aacttcatgg ggctcatcga ggtggtgacg ggctacgtga agatccgcca 2401 ttctcatgcc ttggtctcct tgtccttcct aaaaaacctt cgcctcatcc taggagagga 2461 gcagctagaa gggaattact ccttctacgt cctcgacaac cagaacttgc agcaactgtg 2521 ggactgggac caccgcaacc tgaccatcaa agcagggaaa atgtactttg ctttcaatcc 2581 caaattatgt gtttccgaaa tttaccgcat ggaggaagtg acggggacta aagggcgcca 2641 aagcaaaggg gacataaaca ccaggaacaa cggggagaga gcctcctgtg aaagtgacgt 2701 cctgcatttc acctccacca ccacgtcgaa gaatcgcatc atcataacct ggcaccggta 2761 ccggccccct gactacaggg atctcatcag cttcaccgtt tactacaagg aagcaccctt 2821 taagaatgtc acagagtatg atgggcagga tgcctgcggc tccaacagct ggaacatggt 2881 ggacgtggac ctcccgccca acaaggacgt ggagcccggc atcttactac atgggctgaa 2941 gccctggact cagtacgccg tttacgtcaa ggctgtgacc ctcaccatgg tggagaacga 3001 ccatatccgt ggggccaaga gtgagatctt gtacattcgc accaatgctt cagttccttc 3061 cattcccttg gacgttcttt cagcatcgaa ctcctcttct cagttaatcg tgaagtggaa 3121 ccctccctct ctgcccaacg gcaacctgag ttactacatt gtgcgctggc agcggcagcc 3181 tcaggacggc tacctttacc ggcacaatta ctgctccaaa gacaaaatcc ccatcaggaa 3241 gtatgccgac ggcaccatcg acattgagga ggtcacagag aaccccaaga ctgaggtgtg 3301 tggtggggag aaagggcctt gctgcgcctg ccccaaaact gaagccgaga agcaggccga 3361 gaaggaggag gctgaatacc gcaaagtctt tgagaatttc ctgcacaact ccatcttcgt 3421 gcccagacct gaaaggaagc ggagagatgt catgcaagtg gccaacacca ccatgtccag 3481 ccgaagcagg aacaccacgg ccgcagacac ctacaacatc accgacccgg aagagctgga 3541 gacagagtac cctttctttg agagcagagt ggataacaag gagagaactg tcatttctaa 3601 ccttcggcct ttcacattgt accgcatcga tatccacagc tgcaaccacg aggctgagaa 3661 gctgggctgc agcgcctcca acttcgtctt tgcaaggact atgcccgcag aaggagcaga 3721 tgacattcct gggccagtga cctgggagcc aaggcctgaa aactccatct ttttaaagtg 3781 gccggaacct gagaatccca atggattgat tctaatgtat gaaataaaat acggatcaca 3841 agttgaggat cagcgagaat gtgtgtccag acaggaatac aggaagtatg gaggggccaa 3901 gctaaaccgg ctaaacccgg ggaactacac agcccggatt caggccacat ctctctctgg 3961 gaatgggtcg tggacagatc ctgtgttctt ctatgtccag gccaaaacag gatatgaaaa 4021 cttcatccat ctgatcatcg ctctgcccgt cgctgtcctg ttgatcgtgg gagggttggt 4081 gattatgctg tacgtcttcc atagaaagag aaataacagc aggctgggga atggagtgct 4141 gtatgcctct gtgaacccgg agtacttcag cgctgctgat gtgtacgttc ctgatgagtg 4201 ggaggtggct cgggagaaga tcaccatgag ccgggaactt gggcaggggt cgtttgggat 4261 ggtctatgaa ggagttgcca agggtgtggt gaaagatgaa cctgaaacca gagtggccat 4321 taaaacagtg aacgaggccg caagcatgcg tgagaggatt gagtttctca acgaagcttc 4381 tgtgatgaag gagctcaatt gtcaccatgt ggtgcgattg ctgggtgtgg tgtcccaagg 4441 ccagccaaca ctggtcatca tggaactgat gacacggggc gatctcaaaa gttatctccg 4501 gtctctgagg ccagaaatgg agaataatcc agtcctagca cctccaagcc tgagcaagat 4561 gattcagatg gccggagaga ttgcagacgg catggcatac ctcaacgcca ataagttcgt 4621 ccacagagac cttgctgccc ggaattgcat ggtagccgaa gatttcacag tcaaaatcgg 4681 agattttggt atgacgcgag atatctatga gacagactat taccggaaag gagggaaagg 4741 gctgctgccc gtgcgctgga tgtctcctga gtccctcaag gatggagtct tcaccactta 4801 ctcggacgtc tggtccttcg gggtcgtcct ctgggagatc gccacactgg ccgagcagcc 4861 ctaccagggc ttgtccaacg agcaagtcct tcgcttcgtc atggagggcg gccttctgga 4921 caagccagac aactgtcctg acatgctgtt tgaactgatg cgcatgtgct ggcagtataa 4981 ccccaagatg aggccttcct tcctggagat catcagcagc atcaaagagg agatggagcc 5041 tggcttccgg gaggtctcct tctactacag cgaggagaac aagctgcccg agccggagga 5101 gctggacctg gagccagaga acatggagag cgtccccctg gacccctcgg cctcctcgtc 5161 ctccctgcca ctgcccgaca gacactcagg acacaaggcc gagaacggcc ccggccctgg 5221 ggtgctggtc ctccgcgcca gcttcgacga gagacagcct tacgcccaca tgaacggggg 5281 ccgcaagaac gagcgggcct tgccgctgcc ccagtcttcg acctgctaga acccagcttt 5341 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg 5401 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta 5461 gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 5521 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 5581 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 5641 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 5701 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 5761 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 5821 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 5881 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 5941 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca 6001 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca 6061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 6121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 6181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 6241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 6301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 6361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 6421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 6481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 6541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 6601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 6661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 6721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat 6781 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 6841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 6901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 6961 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 7021 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 7081 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 7141 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 7201 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 7261 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 7321 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc 7381 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7441 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 7501 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 7561 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 7621 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 7681 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 7741 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc 7801 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 7861 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 7921 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 7981 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 8041 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 8101 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 8161 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 8221 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 8281 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 8341 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 8401 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 8461 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 8521 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 8581 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 8641 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 8701 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 8761 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 8821 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 8881 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 8941 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 9001 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 9061 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 9121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 9181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 9241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 9301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 9361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 9421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 9481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 9541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 9601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 9661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 9721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 9781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 9841 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 9901 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 9961 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 10021 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 10081 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt 10141 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 10201 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 10261 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 10321 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 10381 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct 10441 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg 10501 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa 10561 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg 10621 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca 10681 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta 10741 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg 10801 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt 10861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 10921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 10981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 11041 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 11101 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 11161 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 11221 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 11281 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 11341 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 11401 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 11461 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 11521 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg 11581 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 11641 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 11701 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 11761 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 11821 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 11881 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 11941 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 12001 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 12061 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 12121 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 12181 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 12241 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 12301 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 12361 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc 12421 cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt 12481 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac 12541 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag 12601 tttggactag tcgtga //